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Antidepressant-like effects of the hydroalcoholic extracts of Hemerocallis citrina and its potential active components.

Du B, Tang X, Liu F, Zhang C, Zhao G, Ren F, Leng X - BMC Complement Altern Med (2014)

Bottom Line: The antidepressant-like effects of hydroalcoholic H. citrina extracts were mainly related to flavonoids, especially rutin and hesperidin.The antidepressant-like effects of hydroalcoholic H. citrina extracts are mainly related to flavonoids, especially rutin and hesperidin.The active extract is toxicologically safe for oral administration.

View Article: PubMed Central - PubMed

Affiliation: CAU & ACC Joint-Laboratory of Space Food, College of Food Science & Nutritional Engineering, Key Laboratory of Functional Dairy Science of Beijing and Ministry of Education, Beijing Higher Institution Engineering Research Center of Animal Product, Beijing Dairy Industry Innovation Team, China Agricultural University, No,17 Qinghua East Road, Haidian, Beijing 100083, People's Republic of China. gzhao1000@163.com.

ABSTRACT

Background: Herbal therapies are potential alternatives and adjuncts for depression treatment. The present study aims to investigate the antidepressant-like effects of hydroalcoholic Hemerocallis citrina extracts and its potential neuropharmacological components.

Methods: Hydroalcoholic H. citrina extracts were phytochemically analyzed. Behavioral models, including tail suspension tests and open field tests, were performed to evaluate the antidepressant-like effects of the extracts. A possible mechanism was explored by analyzing brain monoamine neurotransmitters. Toxicity and histopathological analyses were performed to determine whether or not the extracts are safe for oral administration.

Results: The antidepressant-like effects of hydroalcoholic H. citrina extracts were mainly related to flavonoids, especially rutin and hesperidin. The extract prepared using 75% ethanol (i.e., HCE75) exhibited the highest active flavonoid content and activity. Orally administered 400 mg/kg of HCE75 significantly induced an antidepressant-like effect, whereas the combination of equivalent rutin and hesperidin dosages exhibited the same profiles. Isobologram analysis showed sub-additive antidepressant interactions between rutin and hesperidin. HCE75 (400 mg/kg, p.o.) increased the serotonin and dopamine levels in the central nervous system. Mortality and lesions were not observed upon oral administration of up to 5000 mg/kg HCE75.

Conclusions: The antidepressant-like effects of hydroalcoholic H. citrina extracts are mainly related to flavonoids, especially rutin and hesperidin. The serotonergic and dopaminergic systems may have major roles. The active extract is toxicologically safe for oral administration.

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Related in: MedlinePlus

Representative histopathological sections of the (A) liver, (B) kidney, and (C) spleen of the mice treated with HCE75 (5000 mg/kg, p.o.) once a day for 1 d (acute treatment) or 21 consecutive days (chronic treatment). The control group was not treated. Insignificant lesions were observed in the experiments. Hematoxylin–eosin stains, 400 × original magnification.
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Fig8: Representative histopathological sections of the (A) liver, (B) kidney, and (C) spleen of the mice treated with HCE75 (5000 mg/kg, p.o.) once a day for 1 d (acute treatment) or 21 consecutive days (chronic treatment). The control group was not treated. Insignificant lesions were observed in the experiments. Hematoxylin–eosin stains, 400 × original magnification.

Mentions: Figure 8 shows that the histopathological examination has insignificant differences. The results reveal that HCE75 (5000 mg/kg, p.o.) administration once a day either for 1 d or 21 consecutive days does not cause lesions on the examined physiological organs. Oral HCE75 administration per se could thus be considered toxicologically safe.Figure 8


Antidepressant-like effects of the hydroalcoholic extracts of Hemerocallis citrina and its potential active components.

Du B, Tang X, Liu F, Zhang C, Zhao G, Ren F, Leng X - BMC Complement Altern Med (2014)

Representative histopathological sections of the (A) liver, (B) kidney, and (C) spleen of the mice treated with HCE75 (5000 mg/kg, p.o.) once a day for 1 d (acute treatment) or 21 consecutive days (chronic treatment). The control group was not treated. Insignificant lesions were observed in the experiments. Hematoxylin–eosin stains, 400 × original magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4289322&req=5

Fig8: Representative histopathological sections of the (A) liver, (B) kidney, and (C) spleen of the mice treated with HCE75 (5000 mg/kg, p.o.) once a day for 1 d (acute treatment) or 21 consecutive days (chronic treatment). The control group was not treated. Insignificant lesions were observed in the experiments. Hematoxylin–eosin stains, 400 × original magnification.
Mentions: Figure 8 shows that the histopathological examination has insignificant differences. The results reveal that HCE75 (5000 mg/kg, p.o.) administration once a day either for 1 d or 21 consecutive days does not cause lesions on the examined physiological organs. Oral HCE75 administration per se could thus be considered toxicologically safe.Figure 8

Bottom Line: The antidepressant-like effects of hydroalcoholic H. citrina extracts were mainly related to flavonoids, especially rutin and hesperidin.The antidepressant-like effects of hydroalcoholic H. citrina extracts are mainly related to flavonoids, especially rutin and hesperidin.The active extract is toxicologically safe for oral administration.

View Article: PubMed Central - PubMed

Affiliation: CAU & ACC Joint-Laboratory of Space Food, College of Food Science & Nutritional Engineering, Key Laboratory of Functional Dairy Science of Beijing and Ministry of Education, Beijing Higher Institution Engineering Research Center of Animal Product, Beijing Dairy Industry Innovation Team, China Agricultural University, No,17 Qinghua East Road, Haidian, Beijing 100083, People's Republic of China. gzhao1000@163.com.

ABSTRACT

Background: Herbal therapies are potential alternatives and adjuncts for depression treatment. The present study aims to investigate the antidepressant-like effects of hydroalcoholic Hemerocallis citrina extracts and its potential neuropharmacological components.

Methods: Hydroalcoholic H. citrina extracts were phytochemically analyzed. Behavioral models, including tail suspension tests and open field tests, were performed to evaluate the antidepressant-like effects of the extracts. A possible mechanism was explored by analyzing brain monoamine neurotransmitters. Toxicity and histopathological analyses were performed to determine whether or not the extracts are safe for oral administration.

Results: The antidepressant-like effects of hydroalcoholic H. citrina extracts were mainly related to flavonoids, especially rutin and hesperidin. The extract prepared using 75% ethanol (i.e., HCE75) exhibited the highest active flavonoid content and activity. Orally administered 400 mg/kg of HCE75 significantly induced an antidepressant-like effect, whereas the combination of equivalent rutin and hesperidin dosages exhibited the same profiles. Isobologram analysis showed sub-additive antidepressant interactions between rutin and hesperidin. HCE75 (400 mg/kg, p.o.) increased the serotonin and dopamine levels in the central nervous system. Mortality and lesions were not observed upon oral administration of up to 5000 mg/kg HCE75.

Conclusions: The antidepressant-like effects of hydroalcoholic H. citrina extracts are mainly related to flavonoids, especially rutin and hesperidin. The serotonergic and dopaminergic systems may have major roles. The active extract is toxicologically safe for oral administration.

Show MeSH
Related in: MedlinePlus