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Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment.

Juang CL, Yang FS, Hsieh MS, Tseng HY, Chen SC, Wen HC - BMC Complement Altern Med (2014)

Bottom Line: The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Optometry, Yuanpei University, Hsinchu 30015, Taiwan. sjwen@mail.ypu.edu.tw.

ABSTRACT

Background: Spinal cord injury (SCI)-induced secondary oxidative stress associates with a clinical complication and high mortality. Treatments to improve the neurological outcome of secondary injury are considered as important issues. The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.

Methods: The anti-oxidation properties were tested in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Rat liver cells (clone-9) were treated with various doses of TDE (0 ~ 50 μg/ml) before exposed to 250 μM H2O2 and cell survival was determined by MTT and LDH assays. We performed water apparent diffusion coefficient (ADC) map in MR techniques to investigate the efficacy of TDE treatment on SCI animal model. We performed T5 laminectomy and compression (50 g, 1 min) to induce SCI. PHILIP 3.0 T MRI was used to image 24 male Sprague-Dawley rats weighing 280-320 g. Rats were randomly divided into three groups: sham group, SCI group, SCI treated with TDE group. The MRI images were taken and ADC were acquired before and after of treatment of TDE (50 mg/kg B. W. orally, 5 days) in SCI model.

Results: TDE protected clone-9 cells against H2O2-induced toxicity through DPPH scavenging mechanism. In addition, SCI induced the increase in ADC after 6 hours. TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.

Conclusion: Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

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Related in: MedlinePlus

Time course of spinal cord injury (SCI) on apparent diffusion coefficient (ADC). Rats were induced SCI by T5 laminectomy and compression (50 g, 1 min). ADC map was used to assessment the injured level. Data are presented as mean ± S.E.M of six rats. Significance for the time-dependent effect after SCI treatment was calculated by ANOVA. *P < 0.05, significantly different from untreated control samples.
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Fig4: Time course of spinal cord injury (SCI) on apparent diffusion coefficient (ADC). Rats were induced SCI by T5 laminectomy and compression (50 g, 1 min). ADC map was used to assessment the injured level. Data are presented as mean ± S.E.M of six rats. Significance for the time-dependent effect after SCI treatment was calculated by ANOVA. *P < 0.05, significantly different from untreated control samples.

Mentions: Given that TDE has demonstrated a potential ant-oxidative and free radical scavenging effect in the in vitro system, we are seeking whether this plant extract can be performed to an in vivo system. One of the known in vivo systems with oxidative stress is spinal cord injury [18, 19]. To assist the data evaluation, we also performed ADC to analyze MRI images and examined the SCI grading. At different time points (0, 2, 4, 6 hr) after SCI, rats were anesthetized and analyze the ADC value using MRI. The results showed SCI resulted in significant increase in ADC after 6 hr (Figure 4), suggesting an increase of cell permeability after SCI. To further confirm the SCI injury model was successfully executed, we then performed DTI to evaluate the damage level of fiber tracts after SCI [20]. TDE treatment slightly decreased the ADC level in 1 week after SCI compared with no TDE treatment in animal study (Figure 5).Figure 4


Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment.

Juang CL, Yang FS, Hsieh MS, Tseng HY, Chen SC, Wen HC - BMC Complement Altern Med (2014)

Time course of spinal cord injury (SCI) on apparent diffusion coefficient (ADC). Rats were induced SCI by T5 laminectomy and compression (50 g, 1 min). ADC map was used to assessment the injured level. Data are presented as mean ± S.E.M of six rats. Significance for the time-dependent effect after SCI treatment was calculated by ANOVA. *P < 0.05, significantly different from untreated control samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4289305&req=5

Fig4: Time course of spinal cord injury (SCI) on apparent diffusion coefficient (ADC). Rats were induced SCI by T5 laminectomy and compression (50 g, 1 min). ADC map was used to assessment the injured level. Data are presented as mean ± S.E.M of six rats. Significance for the time-dependent effect after SCI treatment was calculated by ANOVA. *P < 0.05, significantly different from untreated control samples.
Mentions: Given that TDE has demonstrated a potential ant-oxidative and free radical scavenging effect in the in vitro system, we are seeking whether this plant extract can be performed to an in vivo system. One of the known in vivo systems with oxidative stress is spinal cord injury [18, 19]. To assist the data evaluation, we also performed ADC to analyze MRI images and examined the SCI grading. At different time points (0, 2, 4, 6 hr) after SCI, rats were anesthetized and analyze the ADC value using MRI. The results showed SCI resulted in significant increase in ADC after 6 hr (Figure 4), suggesting an increase of cell permeability after SCI. To further confirm the SCI injury model was successfully executed, we then performed DTI to evaluate the damage level of fiber tracts after SCI [20]. TDE treatment slightly decreased the ADC level in 1 week after SCI compared with no TDE treatment in animal study (Figure 5).Figure 4

Bottom Line: The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Optometry, Yuanpei University, Hsinchu 30015, Taiwan. sjwen@mail.ypu.edu.tw.

ABSTRACT

Background: Spinal cord injury (SCI)-induced secondary oxidative stress associates with a clinical complication and high mortality. Treatments to improve the neurological outcome of secondary injury are considered as important issues. The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.

Methods: The anti-oxidation properties were tested in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Rat liver cells (clone-9) were treated with various doses of TDE (0 ~ 50 μg/ml) before exposed to 250 μM H2O2 and cell survival was determined by MTT and LDH assays. We performed water apparent diffusion coefficient (ADC) map in MR techniques to investigate the efficacy of TDE treatment on SCI animal model. We performed T5 laminectomy and compression (50 g, 1 min) to induce SCI. PHILIP 3.0 T MRI was used to image 24 male Sprague-Dawley rats weighing 280-320 g. Rats were randomly divided into three groups: sham group, SCI group, SCI treated with TDE group. The MRI images were taken and ADC were acquired before and after of treatment of TDE (50 mg/kg B. W. orally, 5 days) in SCI model.

Results: TDE protected clone-9 cells against H2O2-induced toxicity through DPPH scavenging mechanism. In addition, SCI induced the increase in ADC after 6 hours. TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.

Conclusion: Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

Show MeSH
Related in: MedlinePlus