Limits...
Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment.

Juang CL, Yang FS, Hsieh MS, Tseng HY, Chen SC, Wen HC - BMC Complement Altern Med (2014)

Bottom Line: The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Optometry, Yuanpei University, Hsinchu 30015, Taiwan. sjwen@mail.ypu.edu.tw.

ABSTRACT

Background: Spinal cord injury (SCI)-induced secondary oxidative stress associates with a clinical complication and high mortality. Treatments to improve the neurological outcome of secondary injury are considered as important issues. The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.

Methods: The anti-oxidation properties were tested in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Rat liver cells (clone-9) were treated with various doses of TDE (0 ~ 50 μg/ml) before exposed to 250 μM H2O2 and cell survival was determined by MTT and LDH assays. We performed water apparent diffusion coefficient (ADC) map in MR techniques to investigate the efficacy of TDE treatment on SCI animal model. We performed T5 laminectomy and compression (50 g, 1 min) to induce SCI. PHILIP 3.0 T MRI was used to image 24 male Sprague-Dawley rats weighing 280-320 g. Rats were randomly divided into three groups: sham group, SCI group, SCI treated with TDE group. The MRI images were taken and ADC were acquired before and after of treatment of TDE (50 mg/kg B. W. orally, 5 days) in SCI model.

Results: TDE protected clone-9 cells against H2O2-induced toxicity through DPPH scavenging mechanism. In addition, SCI induced the increase in ADC after 6 hours. TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.

Conclusion: Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

Show MeSH

Related in: MedlinePlus

Free radical scavenging profile of TDE expressed as % of DPPH scavenging compared to ascorbic acid. IC50 values for TDE and ascorbic acid were 0.93 ± 0.2 and 0.48 ± 0.1 μg/ml respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4289305&req=5

Fig1: Free radical scavenging profile of TDE expressed as % of DPPH scavenging compared to ascorbic acid. IC50 values for TDE and ascorbic acid were 0.93 ± 0.2 and 0.48 ± 0.1 μg/ml respectively.

Mentions: We first examined the antioxidative effect of TDE by using an in vitro cell free system. We performed DPPH to test the free RSC of TDE. The use of vitamin C was as a positive control. Free radical scavenging profile of TDE was expressed as % of DPPH inhibition compared to ascorbic acid. The result showed that the IC50 values for TDE and ascorbic acid were 0.93 ± 0.20 and 0.48 ± 0.10 μg/ml, respectively (see Figure 1), suggesting a free radical scavenging ability of TDE.Figure 1


Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts treatment.

Juang CL, Yang FS, Hsieh MS, Tseng HY, Chen SC, Wen HC - BMC Complement Altern Med (2014)

Free radical scavenging profile of TDE expressed as % of DPPH scavenging compared to ascorbic acid. IC50 values for TDE and ascorbic acid were 0.93 ± 0.2 and 0.48 ± 0.1 μg/ml respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4289305&req=5

Fig1: Free radical scavenging profile of TDE expressed as % of DPPH scavenging compared to ascorbic acid. IC50 values for TDE and ascorbic acid were 0.93 ± 0.2 and 0.48 ± 0.1 μg/ml respectively.
Mentions: We first examined the antioxidative effect of TDE by using an in vitro cell free system. We performed DPPH to test the free RSC of TDE. The use of vitamin C was as a positive control. Free radical scavenging profile of TDE was expressed as % of DPPH inhibition compared to ascorbic acid. The result showed that the IC50 values for TDE and ascorbic acid were 0.93 ± 0.20 and 0.48 ± 0.10 μg/ml, respectively (see Figure 1), suggesting a free radical scavenging ability of TDE.Figure 1

Bottom Line: The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Optometry, Yuanpei University, Hsinchu 30015, Taiwan. sjwen@mail.ypu.edu.tw.

ABSTRACT

Background: Spinal cord injury (SCI)-induced secondary oxidative stress associates with a clinical complication and high mortality. Treatments to improve the neurological outcome of secondary injury are considered as important issues. The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.

Methods: The anti-oxidation properties were tested in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Rat liver cells (clone-9) were treated with various doses of TDE (0 ~ 50 μg/ml) before exposed to 250 μM H2O2 and cell survival was determined by MTT and LDH assays. We performed water apparent diffusion coefficient (ADC) map in MR techniques to investigate the efficacy of TDE treatment on SCI animal model. We performed T5 laminectomy and compression (50 g, 1 min) to induce SCI. PHILIP 3.0 T MRI was used to image 24 male Sprague-Dawley rats weighing 280-320 g. Rats were randomly divided into three groups: sham group, SCI group, SCI treated with TDE group. The MRI images were taken and ADC were acquired before and after of treatment of TDE (50 mg/kg B. W. orally, 5 days) in SCI model.

Results: TDE protected clone-9 cells against H2O2-induced toxicity through DPPH scavenging mechanism. In addition, SCI induced the increase in ADC after 6 hours. TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.

Conclusion: Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.

Show MeSH
Related in: MedlinePlus