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Effect of LLLT on endothelial cells culture.

Góralczyk K, Szymańska J, Łukowicz M, Drela E, Kotzbach R, Dubiel M, Michalska M, Góralczyk B, Zając A, Rość D - Lasers Med Sci (2014)

Bottom Line: Growth factors as vascular endothelial growth factor (VEGF), produced by the endothelial cells, take an essential part in pathological and physiological angiogenesis.Thus, the aim of the study was to investigate the influence of low-level laser therapy (LLLT) on the proliferation of endothelial cells, secretion of VEGF-A and presence of soluble VEGF receptors (sVEGFR-1 and sVEGFR-2) in the medium after in vitro culture.Isolated human umbilical vein endothelial cells (HUVECs) were irradiated using a diode laser at a wavelength of 635 nm and power density of 1,875 mW/cm(2).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathophysiology, Collegium Medicum in Bydgoszcz, The Nicolaus Copernicus University in Toruń (NCU), Ul. M. Skłodowskiej-Curie 9, 85-094, Bydgoszcz, Poland, krzyg@cm.umk.pl.

ABSTRACT
Growth factors as vascular endothelial growth factor (VEGF), produced by the endothelial cells, take an essential part in pathological and physiological angiogenesis. The possibility of angiogenesis modulation by application of laser radiation may contribute to the improvement of its use in this process. Thus, the aim of the study was to investigate the influence of low-level laser therapy (LLLT) on the proliferation of endothelial cells, secretion of VEGF-A and presence of soluble VEGF receptors (sVEGFR-1 and sVEGFR-2) in the medium after in vitro culture. Isolated human umbilical vein endothelial cells (HUVECs) were irradiated using a diode laser at a wavelength of 635 nm and power density of 1,875 mW/cm(2). Depending on radiation energy density, the experiment was conducted in four groups: I 0 J/cm(2) (control group), II 2 J/cm(2), III 4 J/cm(2), and IV 8 J/cm(2). The use of laser radiation wavelength of 635 nm, was associated with a statistically significant increase in proliferation of endothelial cells (p = 0.0041). Moreover, at 635-nm wavelength, all doses of radiation significantly reduced the concentration of sVEGFR-1 (p = 0.0197).

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Concentration of sVEGFR-2 in the supernatant, depending on LLLT dose with the wavelength of 635 nm (p = 0.23). Values are expressed as the mean ± SEM
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Fig4: Concentration of sVEGFR-2 in the supernatant, depending on LLLT dose with the wavelength of 635 nm (p = 0.23). Values are expressed as the mean ± SEM

Mentions: Figure 1 presents the effect of laser radiation with the wavelength of 635 nm (1,875 mW/cm2) on number of endothelial cells depending on rising energy dose of radiation. Energy doses of 2, 4, and 8 J/cm2 significantly increased number of cells (p = 0.0041, significant differences between groups: I vs. II, III, and IV). The highest value was observed at an energy dose of 2 and 4 J/cm2 and was about 23 % higher than in the control group. The concentrations of VEGF-A and its soluble receptors sVEGFR-1 and sVEGFR-2 are presented in Figs. 2, 3, and 4. The use of laser radiation of 635 nm (1,875 mW/cm2) was associated with a statistically significant lower concentration of sVEGFR-1. The results of analysis of variance were as follows: VEGF-A p = 0.0808, sVEGFR-1 p = 0.0197 (significant differences between groups I vs. II, III, and IV), and sVEGFR-2 p = 0.2340. Statistically significant p values for post hoc test were presented on the figures.Fig. 1


Effect of LLLT on endothelial cells culture.

Góralczyk K, Szymańska J, Łukowicz M, Drela E, Kotzbach R, Dubiel M, Michalska M, Góralczyk B, Zając A, Rość D - Lasers Med Sci (2014)

Concentration of sVEGFR-2 in the supernatant, depending on LLLT dose with the wavelength of 635 nm (p = 0.23). Values are expressed as the mean ± SEM
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4289014&req=5

Fig4: Concentration of sVEGFR-2 in the supernatant, depending on LLLT dose with the wavelength of 635 nm (p = 0.23). Values are expressed as the mean ± SEM
Mentions: Figure 1 presents the effect of laser radiation with the wavelength of 635 nm (1,875 mW/cm2) on number of endothelial cells depending on rising energy dose of radiation. Energy doses of 2, 4, and 8 J/cm2 significantly increased number of cells (p = 0.0041, significant differences between groups: I vs. II, III, and IV). The highest value was observed at an energy dose of 2 and 4 J/cm2 and was about 23 % higher than in the control group. The concentrations of VEGF-A and its soluble receptors sVEGFR-1 and sVEGFR-2 are presented in Figs. 2, 3, and 4. The use of laser radiation of 635 nm (1,875 mW/cm2) was associated with a statistically significant lower concentration of sVEGFR-1. The results of analysis of variance were as follows: VEGF-A p = 0.0808, sVEGFR-1 p = 0.0197 (significant differences between groups I vs. II, III, and IV), and sVEGFR-2 p = 0.2340. Statistically significant p values for post hoc test were presented on the figures.Fig. 1

Bottom Line: Growth factors as vascular endothelial growth factor (VEGF), produced by the endothelial cells, take an essential part in pathological and physiological angiogenesis.Thus, the aim of the study was to investigate the influence of low-level laser therapy (LLLT) on the proliferation of endothelial cells, secretion of VEGF-A and presence of soluble VEGF receptors (sVEGFR-1 and sVEGFR-2) in the medium after in vitro culture.Isolated human umbilical vein endothelial cells (HUVECs) were irradiated using a diode laser at a wavelength of 635 nm and power density of 1,875 mW/cm(2).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathophysiology, Collegium Medicum in Bydgoszcz, The Nicolaus Copernicus University in Toruń (NCU), Ul. M. Skłodowskiej-Curie 9, 85-094, Bydgoszcz, Poland, krzyg@cm.umk.pl.

ABSTRACT
Growth factors as vascular endothelial growth factor (VEGF), produced by the endothelial cells, take an essential part in pathological and physiological angiogenesis. The possibility of angiogenesis modulation by application of laser radiation may contribute to the improvement of its use in this process. Thus, the aim of the study was to investigate the influence of low-level laser therapy (LLLT) on the proliferation of endothelial cells, secretion of VEGF-A and presence of soluble VEGF receptors (sVEGFR-1 and sVEGFR-2) in the medium after in vitro culture. Isolated human umbilical vein endothelial cells (HUVECs) were irradiated using a diode laser at a wavelength of 635 nm and power density of 1,875 mW/cm(2). Depending on radiation energy density, the experiment was conducted in four groups: I 0 J/cm(2) (control group), II 2 J/cm(2), III 4 J/cm(2), and IV 8 J/cm(2). The use of laser radiation wavelength of 635 nm, was associated with a statistically significant increase in proliferation of endothelial cells (p = 0.0041). Moreover, at 635-nm wavelength, all doses of radiation significantly reduced the concentration of sVEGFR-1 (p = 0.0197).

Show MeSH