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Versatile and enhanced tumour modelling in mice via somatic cell transduction.

Rodriguez E, Mannion L, D'Santos P, Griffiths M, Arends MJ, Brindle KM, Lyons SK - J. Pathol. (2014)

Bottom Line: The vectors deliver a tamoxifen-inducible and self-inactivating Cre recombinase, conditional bioluminescent and fluorescent proteins and an shRNA component.We also show that a variant vector expressing shRNA alters tumour growth dynamics and the histological grade associated with the inherited genotype.They should also be useful for inducing imageable tumours in organs other than the lung, provided that the inherited conditional genotype is sufficiently penetrant.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Imaging, CRUK Cambridge Institute, University of Cambridge, UK.

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Related in: MedlinePlus

(a) Representative end stage bioluminescence images of mice (in vivo) and lungs (ex vivo) of: (1) K; (2) KP; (3) K+miR; and (4) wild-type mice, following infection with LV–indLS2 (3 min; large bin acquisition in all cases). (b) End-stage histology of lungs from a KP mouse at day 270 after LV–indLS2 infection, showing H&E staining and α-RFP immunohistochemistry
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fig04: (a) Representative end stage bioluminescence images of mice (in vivo) and lungs (ex vivo) of: (1) K; (2) KP; (3) K+miR; and (4) wild-type mice, following infection with LV–indLS2 (3 min; large bin acquisition in all cases). (b) End-stage histology of lungs from a KP mouse at day 270 after LV–indLS2 infection, showing H&E staining and α-RFP immunohistochemistry

Mentions: The measured lung bioluminescence from K mice transduced with LV-indLSmiR1224 developed in a manner intermediate to that of to its inherited genotype and KP mice (Figure 3). Unambiguous lung signal appeared approximately 1 month earlier in this cohort than in K or KP mice, however; once bioluminescence was established (after ˜100 days), the tumours did not appear to develop as quickly as those from the KP cohort. Histological analysis of lung tumours from these mice (Figures 4, S4, S5 and Table1) at 7 months post-Cre induction showed that the tumour phenotype was broadly similar, but intermediate in terms of grade, to mice with inherited K and KP genotypes. In addition to developing tumours faster than K mice, K+mir1224 mice developed more frequent high-grade adenomas, but not as frequently as KP mice.


Versatile and enhanced tumour modelling in mice via somatic cell transduction.

Rodriguez E, Mannion L, D'Santos P, Griffiths M, Arends MJ, Brindle KM, Lyons SK - J. Pathol. (2014)

(a) Representative end stage bioluminescence images of mice (in vivo) and lungs (ex vivo) of: (1) K; (2) KP; (3) K+miR; and (4) wild-type mice, following infection with LV–indLS2 (3 min; large bin acquisition in all cases). (b) End-stage histology of lungs from a KP mouse at day 270 after LV–indLS2 infection, showing H&E staining and α-RFP immunohistochemistry
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4288983&req=5

fig04: (a) Representative end stage bioluminescence images of mice (in vivo) and lungs (ex vivo) of: (1) K; (2) KP; (3) K+miR; and (4) wild-type mice, following infection with LV–indLS2 (3 min; large bin acquisition in all cases). (b) End-stage histology of lungs from a KP mouse at day 270 after LV–indLS2 infection, showing H&E staining and α-RFP immunohistochemistry
Mentions: The measured lung bioluminescence from K mice transduced with LV-indLSmiR1224 developed in a manner intermediate to that of to its inherited genotype and KP mice (Figure 3). Unambiguous lung signal appeared approximately 1 month earlier in this cohort than in K or KP mice, however; once bioluminescence was established (after ˜100 days), the tumours did not appear to develop as quickly as those from the KP cohort. Histological analysis of lung tumours from these mice (Figures 4, S4, S5 and Table1) at 7 months post-Cre induction showed that the tumour phenotype was broadly similar, but intermediate in terms of grade, to mice with inherited K and KP genotypes. In addition to developing tumours faster than K mice, K+mir1224 mice developed more frequent high-grade adenomas, but not as frequently as KP mice.

Bottom Line: The vectors deliver a tamoxifen-inducible and self-inactivating Cre recombinase, conditional bioluminescent and fluorescent proteins and an shRNA component.We also show that a variant vector expressing shRNA alters tumour growth dynamics and the histological grade associated with the inherited genotype.They should also be useful for inducing imageable tumours in organs other than the lung, provided that the inherited conditional genotype is sufficiently penetrant.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Imaging, CRUK Cambridge Institute, University of Cambridge, UK.

Show MeSH
Related in: MedlinePlus