Versatile and enhanced tumour modelling in mice via somatic cell transduction.
Bottom Line: The vectors deliver a tamoxifen-inducible and self-inactivating Cre recombinase, conditional bioluminescent and fluorescent proteins and an shRNA component.We also show that a variant vector expressing shRNA alters tumour growth dynamics and the histological grade associated with the inherited genotype.They should also be useful for inducing imageable tumours in organs other than the lung, provided that the inherited conditional genotype is sufficiently penetrant.
Affiliation: Department of Molecular Imaging, CRUK Cambridge Institute, University of Cambridge, UK.Show MeSH
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Mentions: The measured lung bioluminescence from K mice transduced with LV-indLSmiR1224 developed in a manner intermediate to that of to its inherited genotype and KP mice (Figure 3). Unambiguous lung signal appeared approximately 1 month earlier in this cohort than in K or KP mice, however; once bioluminescence was established (after ˜100 days), the tumours did not appear to develop as quickly as those from the KP cohort. Histological analysis of lung tumours from these mice (Figures 4, S4, S5 and Table1) at 7 months post-Cre induction showed that the tumour phenotype was broadly similar, but intermediate in terms of grade, to mice with inherited K and KP genotypes. In addition to developing tumours faster than K mice, K+mir1224 mice developed more frequent high-grade adenomas, but not as frequently as KP mice.
Affiliation: Department of Molecular Imaging, CRUK Cambridge Institute, University of Cambridge, UK.