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Flower development of Phalaenopsis orchid involves functionally divergent SEPALLATA-like genes.

Pan ZJ, Chen YY, Du JS, Chen YY, Chung MC, Tsai WC, Wang CN, Chen HH - New Phytol. (2014)

Bottom Line: The tepal became a leaf-like organ when PeSEP3 was silenced by virus-induced silencing, with alterations in epidermis identity and contents of anthocyanin and chlorophyll.Silencing of the E-class genes PeSEP2 and PeSEP3 resulted in the downregulation of B-class PeMADS2-6 genes, which indicates an association of PeSEP functions and B-class gene expression.These findings reveal the important roles of PeSEP in Phalaenopsis floral organ formation throughout the developmental process by the formation of various multiple protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan.

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Related in: MedlinePlus

Ultrastructures on epidermal cells of flowers of PhalaenopsisOX Red Shoes. (a–j) Ultrastructures of protuberance on the adaxial conical-shaped epidermal cells (a–e) and epicuticular wax crystals on the surface of abaxial convex-shaped epidermal cells (f–j). (k–p) Sudan IV staining of secreted lipid granules on adaxial conical cells in sepal and petal of mock-treated and PeSEP-silenced flowers. (q–v) Cuticular folding on the lip epidermal cells. Bars: (a–j, q–v) 10 μm; (k–p) 100 μm.
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fig08: Ultrastructures on epidermal cells of flowers of PhalaenopsisOX Red Shoes. (a–j) Ultrastructures of protuberance on the adaxial conical-shaped epidermal cells (a–e) and epicuticular wax crystals on the surface of abaxial convex-shaped epidermal cells (f–j). (k–p) Sudan IV staining of secreted lipid granules on adaxial conical cells in sepal and petal of mock-treated and PeSEP-silenced flowers. (q–v) Cuticular folding on the lip epidermal cells. Bars: (a–j, q–v) 10 μm; (k–p) 100 μm.

Mentions: To determine whether floral organs of the PeSEP-silenced flowers were affected at the cellular level, we observed perianth epidermal cell shapes under cryo-SEM, indicated as the aspect ratio β, the ratio of the width to height of epidermal cell curvature (Fig.6e). In addition to the decreased cell size, cell shape and several ultrastructures were altered in epidermal cells of PeSEP-silenced flowers (Figs8). Among the three PeSEP-silenced lines, PeSEP3- and PeSEP2/3-silenced flowers showed the most severe defects in the overall cellular changes, whereas these changes in PeSEP2-silenced flowers were minor.


Flower development of Phalaenopsis orchid involves functionally divergent SEPALLATA-like genes.

Pan ZJ, Chen YY, Du JS, Chen YY, Chung MC, Tsai WC, Wang CN, Chen HH - New Phytol. (2014)

Ultrastructures on epidermal cells of flowers of PhalaenopsisOX Red Shoes. (a–j) Ultrastructures of protuberance on the adaxial conical-shaped epidermal cells (a–e) and epicuticular wax crystals on the surface of abaxial convex-shaped epidermal cells (f–j). (k–p) Sudan IV staining of secreted lipid granules on adaxial conical cells in sepal and petal of mock-treated and PeSEP-silenced flowers. (q–v) Cuticular folding on the lip epidermal cells. Bars: (a–j, q–v) 10 μm; (k–p) 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4288972&req=5

fig08: Ultrastructures on epidermal cells of flowers of PhalaenopsisOX Red Shoes. (a–j) Ultrastructures of protuberance on the adaxial conical-shaped epidermal cells (a–e) and epicuticular wax crystals on the surface of abaxial convex-shaped epidermal cells (f–j). (k–p) Sudan IV staining of secreted lipid granules on adaxial conical cells in sepal and petal of mock-treated and PeSEP-silenced flowers. (q–v) Cuticular folding on the lip epidermal cells. Bars: (a–j, q–v) 10 μm; (k–p) 100 μm.
Mentions: To determine whether floral organs of the PeSEP-silenced flowers were affected at the cellular level, we observed perianth epidermal cell shapes under cryo-SEM, indicated as the aspect ratio β, the ratio of the width to height of epidermal cell curvature (Fig.6e). In addition to the decreased cell size, cell shape and several ultrastructures were altered in epidermal cells of PeSEP-silenced flowers (Figs8). Among the three PeSEP-silenced lines, PeSEP3- and PeSEP2/3-silenced flowers showed the most severe defects in the overall cellular changes, whereas these changes in PeSEP2-silenced flowers were minor.

Bottom Line: The tepal became a leaf-like organ when PeSEP3 was silenced by virus-induced silencing, with alterations in epidermis identity and contents of anthocyanin and chlorophyll.Silencing of the E-class genes PeSEP2 and PeSEP3 resulted in the downregulation of B-class PeMADS2-6 genes, which indicates an association of PeSEP functions and B-class gene expression.These findings reveal the important roles of PeSEP in Phalaenopsis floral organ formation throughout the developmental process by the formation of various multiple protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan.

Show MeSH
Related in: MedlinePlus