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Flower development of Phalaenopsis orchid involves functionally divergent SEPALLATA-like genes.

Pan ZJ, Chen YY, Du JS, Chen YY, Chung MC, Tsai WC, Wang CN, Chen HH - New Phytol. (2014)

Bottom Line: The tepal became a leaf-like organ when PeSEP3 was silenced by virus-induced silencing, with alterations in epidermis identity and contents of anthocyanin and chlorophyll.Silencing of the E-class genes PeSEP2 and PeSEP3 resulted in the downregulation of B-class PeMADS2-6 genes, which indicates an association of PeSEP functions and B-class gene expression.These findings reveal the important roles of PeSEP in Phalaenopsis floral organ formation throughout the developmental process by the formation of various multiple protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan.

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Cellular characterization of the flowers of PeSEP-silenced PhalaenopsisOX Red Shoes. (a) Width of fully developed flowers. n = 6 flowers. (b–e) Density, height, width and aspect ratio β (width/height of epidermal cell curvature) of epidermal cells on adaxial and abaxial sides in mock-treated and PeSEP-silenced flowers. Total number of measurements for each parameter = 15. Error bars: ± SD.
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fig06: Cellular characterization of the flowers of PeSEP-silenced PhalaenopsisOX Red Shoes. (a) Width of fully developed flowers. n = 6 flowers. (b–e) Density, height, width and aspect ratio β (width/height of epidermal cell curvature) of epidermal cells on adaxial and abaxial sides in mock-treated and PeSEP-silenced flowers. Total number of measurements for each parameter = 15. Error bars: ± SD.

Mentions: To determine whether the identities of floral organs of the three PeSEP-silenced lines were affected, we quantified several characteristics, including flower size, cell density, shape, height and length (Fig.6). The mean flower size was smaller in PeSEP3- and PeSEP2/3-silenced plants than in mock-treated and PeSEP2-silenced plants (Fig.6a), whereas the epidermal cell size was decreased in both height and width in sepals and petals (Fig.6c,d), together with increased cell density (Fig.6b). Although the PeSEP2-silenced floral morphological features, especially the lip and column, were almost unaffected and indistinguishable from mock-treated features (Fig.5p,t), we observed slight changes in the epidermis. Similar phenotype changes were observed in the independent experiment with P. I-Hsin Sunrise Cinderella (Figs S4, S5). Therefore, morphological changes were stronger for PeSEP3- than PeSEP2/3-silenced plants. No noticeable changes in PeSEP2-silenced plants suggested that PeSEP2 has redundant functions in floral organ identity.


Flower development of Phalaenopsis orchid involves functionally divergent SEPALLATA-like genes.

Pan ZJ, Chen YY, Du JS, Chen YY, Chung MC, Tsai WC, Wang CN, Chen HH - New Phytol. (2014)

Cellular characterization of the flowers of PeSEP-silenced PhalaenopsisOX Red Shoes. (a) Width of fully developed flowers. n = 6 flowers. (b–e) Density, height, width and aspect ratio β (width/height of epidermal cell curvature) of epidermal cells on adaxial and abaxial sides in mock-treated and PeSEP-silenced flowers. Total number of measurements for each parameter = 15. Error bars: ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4288972&req=5

fig06: Cellular characterization of the flowers of PeSEP-silenced PhalaenopsisOX Red Shoes. (a) Width of fully developed flowers. n = 6 flowers. (b–e) Density, height, width and aspect ratio β (width/height of epidermal cell curvature) of epidermal cells on adaxial and abaxial sides in mock-treated and PeSEP-silenced flowers. Total number of measurements for each parameter = 15. Error bars: ± SD.
Mentions: To determine whether the identities of floral organs of the three PeSEP-silenced lines were affected, we quantified several characteristics, including flower size, cell density, shape, height and length (Fig.6). The mean flower size was smaller in PeSEP3- and PeSEP2/3-silenced plants than in mock-treated and PeSEP2-silenced plants (Fig.6a), whereas the epidermal cell size was decreased in both height and width in sepals and petals (Fig.6c,d), together with increased cell density (Fig.6b). Although the PeSEP2-silenced floral morphological features, especially the lip and column, were almost unaffected and indistinguishable from mock-treated features (Fig.5p,t), we observed slight changes in the epidermis. Similar phenotype changes were observed in the independent experiment with P. I-Hsin Sunrise Cinderella (Figs S4, S5). Therefore, morphological changes were stronger for PeSEP3- than PeSEP2/3-silenced plants. No noticeable changes in PeSEP2-silenced plants suggested that PeSEP2 has redundant functions in floral organ identity.

Bottom Line: The tepal became a leaf-like organ when PeSEP3 was silenced by virus-induced silencing, with alterations in epidermis identity and contents of anthocyanin and chlorophyll.Silencing of the E-class genes PeSEP2 and PeSEP3 resulted in the downregulation of B-class PeMADS2-6 genes, which indicates an association of PeSEP functions and B-class gene expression.These findings reveal the important roles of PeSEP in Phalaenopsis floral organ formation throughout the developmental process by the formation of various multiple protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan.

Show MeSH
Related in: MedlinePlus