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vglut2 and gad expression reveal distinct patterns of dual GABAergic versus glutamatergic cotransmitter phenotypes of dopaminergic and noradrenergic neurons in the zebrafish brain.

Filippi A, Mueller T, Driever W - J. Comp. Neurol. (2014)

Bottom Line: Our results show that most dopaminergic neurons also express GABAergic markers, including the dopaminergic groups of the olfactory bulb (homologous to mammalian A16) and the subpallium, the hypothalamic groups (A12, A14), the prethalamic zona incerta group (A13), the preoptic groups (A15), and the pretectal group.All together, our results demonstrate that all catecholaminergic groups in zebrafish are either GABAergic or glutamatergic.We compare our results with those that have been described for mammalian systems, discuss the phenomenon of transmitter dualism in the context of developmental specification of GABAergic and glutamatergic regions in the brain, and put this phenomenon in an evolutionary perspective.

View Article: PubMed Central - PubMed

Affiliation: Developmental Biology, Institute of Biology I, Faculty of Biology, Albert-Ludwigs-University Freiburg, 79104, Freiburg, Germany.

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Localization of gad1b/2 or vglut2 expression and TH immunoreactivity in the medulla oblongata at 30 dpf. A: Overview of section plane shown in B–D (for details, see Fig. 3A legend). B–D3: Fluorescent in situ hybridization to detect gad1b/2 (C; green) or vglut2 (D; green) expression combined with anti-TH immunofluorescence (red). The overview in B is a 10-μm confocal z-projection showing TH-ir noradrenergic neurons of the medulla oblongata (MO) in the hindbrain. These neurons were intermingled but not colocalizing with gad-expressing cells (C; 3-μm projection). Instead, we detected coexpression of TH with vglut2 (D–D3; 6-μm projection). D1–D3 are single-channel enlarged views of the framed area in D. The arrowheads point at examples of coexpression. A magenta–green copy of this figure is available as Supporting Information Figure S7. Scale bar = 50 μm in B–D.
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fig07: Localization of gad1b/2 or vglut2 expression and TH immunoreactivity in the medulla oblongata at 30 dpf. A: Overview of section plane shown in B–D (for details, see Fig. 3A legend). B–D3: Fluorescent in situ hybridization to detect gad1b/2 (C; green) or vglut2 (D; green) expression combined with anti-TH immunofluorescence (red). The overview in B is a 10-μm confocal z-projection showing TH-ir noradrenergic neurons of the medulla oblongata (MO) in the hindbrain. These neurons were intermingled but not colocalizing with gad-expressing cells (C; 3-μm projection). Instead, we detected coexpression of TH with vglut2 (D–D3; 6-μm projection). D1–D3 are single-channel enlarged views of the framed area in D. The arrowheads point at examples of coexpression. A magenta–green copy of this figure is available as Supporting Information Figure S7. Scale bar = 50 μm in B–D.

Mentions: In the medulla oblongata (Fig. 7B), whereas no overlap was observed between TH and gad (Fig. 7C), we clearly detected coexpression of TH with vglut2 in most noradrenergic neurons (Fig. 7D–D3). A minor number of cells, however, were free of vglut2-expression. However, due to the poor quality of the sections obtained for the posterior hindbrain, the data collected for the noradrenergic group of the medulla oblongata were not complete, and we were not able to determine whether those few vglut2-free cells were GABAergic instead.


vglut2 and gad expression reveal distinct patterns of dual GABAergic versus glutamatergic cotransmitter phenotypes of dopaminergic and noradrenergic neurons in the zebrafish brain.

Filippi A, Mueller T, Driever W - J. Comp. Neurol. (2014)

Localization of gad1b/2 or vglut2 expression and TH immunoreactivity in the medulla oblongata at 30 dpf. A: Overview of section plane shown in B–D (for details, see Fig. 3A legend). B–D3: Fluorescent in situ hybridization to detect gad1b/2 (C; green) or vglut2 (D; green) expression combined with anti-TH immunofluorescence (red). The overview in B is a 10-μm confocal z-projection showing TH-ir noradrenergic neurons of the medulla oblongata (MO) in the hindbrain. These neurons were intermingled but not colocalizing with gad-expressing cells (C; 3-μm projection). Instead, we detected coexpression of TH with vglut2 (D–D3; 6-μm projection). D1–D3 are single-channel enlarged views of the framed area in D. The arrowheads point at examples of coexpression. A magenta–green copy of this figure is available as Supporting Information Figure S7. Scale bar = 50 μm in B–D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4288968&req=5

fig07: Localization of gad1b/2 or vglut2 expression and TH immunoreactivity in the medulla oblongata at 30 dpf. A: Overview of section plane shown in B–D (for details, see Fig. 3A legend). B–D3: Fluorescent in situ hybridization to detect gad1b/2 (C; green) or vglut2 (D; green) expression combined with anti-TH immunofluorescence (red). The overview in B is a 10-μm confocal z-projection showing TH-ir noradrenergic neurons of the medulla oblongata (MO) in the hindbrain. These neurons were intermingled but not colocalizing with gad-expressing cells (C; 3-μm projection). Instead, we detected coexpression of TH with vglut2 (D–D3; 6-μm projection). D1–D3 are single-channel enlarged views of the framed area in D. The arrowheads point at examples of coexpression. A magenta–green copy of this figure is available as Supporting Information Figure S7. Scale bar = 50 μm in B–D.
Mentions: In the medulla oblongata (Fig. 7B), whereas no overlap was observed between TH and gad (Fig. 7C), we clearly detected coexpression of TH with vglut2 in most noradrenergic neurons (Fig. 7D–D3). A minor number of cells, however, were free of vglut2-expression. However, due to the poor quality of the sections obtained for the posterior hindbrain, the data collected for the noradrenergic group of the medulla oblongata were not complete, and we were not able to determine whether those few vglut2-free cells were GABAergic instead.

Bottom Line: Our results show that most dopaminergic neurons also express GABAergic markers, including the dopaminergic groups of the olfactory bulb (homologous to mammalian A16) and the subpallium, the hypothalamic groups (A12, A14), the prethalamic zona incerta group (A13), the preoptic groups (A15), and the pretectal group.All together, our results demonstrate that all catecholaminergic groups in zebrafish are either GABAergic or glutamatergic.We compare our results with those that have been described for mammalian systems, discuss the phenomenon of transmitter dualism in the context of developmental specification of GABAergic and glutamatergic regions in the brain, and put this phenomenon in an evolutionary perspective.

View Article: PubMed Central - PubMed

Affiliation: Developmental Biology, Institute of Biology I, Faculty of Biology, Albert-Ludwigs-University Freiburg, 79104, Freiburg, Germany.

Show MeSH