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Effect of Amphotericin B Nanodisks on Leishmania major Infected Mice.

Cole P, Bishop J, Beckstead J, Titus R, Ryan R - Pharm Anal Acta (2014)

Bottom Line: Balb/c and CH3 mice infected with Leishmania major on Day 0 were administered vehicle alone, empty ND or AMB-ND on Day 1 and day 7, via the tail vein.Mice were sacrificed 25 or 50 days post inoculation and tissue histology evaluated.It may be inferred that AMB-ND may be useful for prophylactic and/or treatment of early stage Leishmania spp. infection.

View Article: PubMed Central - HTML - PubMed

Affiliation: Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, CO 80523-1619.

ABSTRACT

Objective: To assess the efficacy of a novel formulation of the polyene antibiotic, amphotericin B (AMB), as therapy for cutaneous leishmaniasis in different mouse strains.

Methods: (AMB), was formulated into water-soluble transport particles, termed nanodisks (ND). Balb/c and CH3 mice infected with Leishmania major on Day 0 were administered vehicle alone, empty ND or AMB-ND on Day 1 and day 7, via the tail vein. Mice were sacrificed 25 or 50 days post inoculation and tissue histology evaluated. Balb/c mice treated with vehicle or empty ND showed signs of severe infection while CH3 mice had less inflammation and fewer parasites. AMB-ND treatment (2 mg/kg) had a marked therapeutic effect on L. major infected Balb/c mice and a discernable therapeutic benefit on CH3 mice.

Conclusions: AMB-ND is efficacious in the treatment of cutaneous leishmaniasis in both susceptible and resistant mouse strains. It may be inferred that AMB-ND may be useful for prophylactic and/or treatment of early stage Leishmania spp. infection.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of a sagittally sectioned foot of a representative CH3 mouse sacrificed 25 days following inoculation with L. major and treatment with AMB-ND. Panel A) Mild focal inflammation (arrows) is present in the subcutis. H&E, bar=500 µm. Panel B). The cellular infiltrate is composed of mixed mononuclear inflammatory cells. H&E, bar=500 µm.
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Figure 4: Photomicrographs of a sagittally sectioned foot of a representative CH3 mouse sacrificed 25 days following inoculation with L. major and treatment with AMB-ND. Panel A) Mild focal inflammation (arrows) is present in the subcutis. H&E, bar=500 µm. Panel B). The cellular infiltrate is composed of mixed mononuclear inflammatory cells. H&E, bar=500 µm.

Mentions: These mice had no increase in thickness of infected feet and no footpad ulceration. Histologic examination at days 25 and 50 PI showed no detectable L. major parasites and mild inflammation (Figures 4A and 4B). Furthermore, there was no change in popliteal lymph nodes, spleens, kidneys, livers, or non-infected right feet.


Effect of Amphotericin B Nanodisks on Leishmania major Infected Mice.

Cole P, Bishop J, Beckstead J, Titus R, Ryan R - Pharm Anal Acta (2014)

Photomicrographs of a sagittally sectioned foot of a representative CH3 mouse sacrificed 25 days following inoculation with L. major and treatment with AMB-ND. Panel A) Mild focal inflammation (arrows) is present in the subcutis. H&E, bar=500 µm. Panel B). The cellular infiltrate is composed of mixed mononuclear inflammatory cells. H&E, bar=500 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4288788&req=5

Figure 4: Photomicrographs of a sagittally sectioned foot of a representative CH3 mouse sacrificed 25 days following inoculation with L. major and treatment with AMB-ND. Panel A) Mild focal inflammation (arrows) is present in the subcutis. H&E, bar=500 µm. Panel B). The cellular infiltrate is composed of mixed mononuclear inflammatory cells. H&E, bar=500 µm.
Mentions: These mice had no increase in thickness of infected feet and no footpad ulceration. Histologic examination at days 25 and 50 PI showed no detectable L. major parasites and mild inflammation (Figures 4A and 4B). Furthermore, there was no change in popliteal lymph nodes, spleens, kidneys, livers, or non-infected right feet.

Bottom Line: Balb/c and CH3 mice infected with Leishmania major on Day 0 were administered vehicle alone, empty ND or AMB-ND on Day 1 and day 7, via the tail vein.Mice were sacrificed 25 or 50 days post inoculation and tissue histology evaluated.It may be inferred that AMB-ND may be useful for prophylactic and/or treatment of early stage Leishmania spp. infection.

View Article: PubMed Central - HTML - PubMed

Affiliation: Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, CO 80523-1619.

ABSTRACT

Objective: To assess the efficacy of a novel formulation of the polyene antibiotic, amphotericin B (AMB), as therapy for cutaneous leishmaniasis in different mouse strains.

Methods: (AMB), was formulated into water-soluble transport particles, termed nanodisks (ND). Balb/c and CH3 mice infected with Leishmania major on Day 0 were administered vehicle alone, empty ND or AMB-ND on Day 1 and day 7, via the tail vein. Mice were sacrificed 25 or 50 days post inoculation and tissue histology evaluated. Balb/c mice treated with vehicle or empty ND showed signs of severe infection while CH3 mice had less inflammation and fewer parasites. AMB-ND treatment (2 mg/kg) had a marked therapeutic effect on L. major infected Balb/c mice and a discernable therapeutic benefit on CH3 mice.

Conclusions: AMB-ND is efficacious in the treatment of cutaneous leishmaniasis in both susceptible and resistant mouse strains. It may be inferred that AMB-ND may be useful for prophylactic and/or treatment of early stage Leishmania spp. infection.

No MeSH data available.


Related in: MedlinePlus