AtMYB93 is a novel negative regulator of lateral root development in Arabidopsis.
Bottom Line: Furthermore, Atmyb93 mutant lateral root development is insensitive to auxin, indicating that AtMYB93 is required for normal auxin responses during lateral root development.We propose that AtMYB93 is part of a novel auxin-induced negative feedback loop stimulated in a select few endodermal cells early during lateral root development, ensuring that lateral roots only develop when absolutely required.Putative AtMYB93 homologues are detected throughout flowering plants and represent promising targets for manipulating root systems in diverse crop species.
Affiliation: School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.Show MeSH
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Mentions: The initial MYB alignment (Fig. 1c) was conducted using ClustalX (Larkin et al., 2007) using the default settings. Alignments were annotated using Boxshade 3.21 (http://www.ch.embnet.org/software/BOX_doc.html), with the fraction of sequences that must agree for shading set at 1.0. For the phylogeny, putative full-length land plant AtMYB93/92/53 homologues were identified using Blastp from fully sequenced land plant genomes via GenBank and Phytozome (Goodstein et al., 2012). Sequences were aligned using ClustalX and the alignment was refined manually in SeaView (Gouy et al., 2010). The phylogenetic tree was calculated using the maximum likelihood algorithm in SeaView on default settings, with 1000 bootstrap replicates. Similar trees were obtained using distance methods. The tree was displayed using TreeViewX (Page, 2002).
Affiliation: School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.