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Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-κB-signaling Pathway.

Kim B, Kim JE, Choi BK, Kim HS - Biomol Ther (Seoul) (2015)

Bottom Line: In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species.The cytokine array results showed that WCE inhibited inflammatory cytokine secretion.These results indicate that WCE may be a promising topical anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Skin & Bio Research, Ellead Co., Ltd., Seongnam 463-824.

ABSTRACT
Water chestnut (Trapa japonica Flerov.) is an annual aquatic plant. In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species. In addition, water chestnut extract (WCE) inhibited lipopolysaccharide (LPS)-induced nitric oxide production and suppressed mRNA and protein expression of the inducible nitric oxide synthase gene. The cytokine array results showed that WCE inhibited inflammatory cytokine secretion. Also, WCE reduced tumor necrosis factor-α-and interleukin-6-induced nuclear factor-αB activity. Furthermore, during sodium lauryl sulfate (SLS)-induced irritation of human skin, WCE reduced SLS-induced skin erythema and improved barrier regeneration. These results indicate that WCE may be a promising topical anti-inflammatory agent.

No MeSH data available.


Related in: MedlinePlus

Effects of WCE on LPS-induced NO and PGE2 production, and iNOS expression. (A) RAW 264.7 cells were treated with 1 μg/mL of LPS alone or in combination with varying concentrations of WCE for a nitrite assay. The extracellular medium containing nitrate was analyzed by a Griess regent system. (B) The PGE2 concentration in the supernatants was determined by ELISA *p<0.05, **p<0.01 compared to the LPS treated group. Values are presented as means ± standard error of the mean (SEM). (C) Western blot analysis of iNOS. β-actin was used as an internal standard. (D) RT-PCR analysis of iNOS. β-actin was used as an internal standard. The PCR products were resolved on a 2% agarose gel.
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f2-bt-23-90: Effects of WCE on LPS-induced NO and PGE2 production, and iNOS expression. (A) RAW 264.7 cells were treated with 1 μg/mL of LPS alone or in combination with varying concentrations of WCE for a nitrite assay. The extracellular medium containing nitrate was analyzed by a Griess regent system. (B) The PGE2 concentration in the supernatants was determined by ELISA *p<0.05, **p<0.01 compared to the LPS treated group. Values are presented as means ± standard error of the mean (SEM). (C) Western blot analysis of iNOS. β-actin was used as an internal standard. (D) RT-PCR analysis of iNOS. β-actin was used as an internal standard. The PCR products were resolved on a 2% agarose gel.

Mentions: Cell viability was not affected by 100 μg/mL WCE (data not shown). Therefore, all subsequent experiments on inflammatory mediator production were conducted with 100 μg/mL WCE. To investigate the anti-inflammatory effects of WCE, we examined its effect on NO production in LPS-induced macrophages. NO production greatly increased after 24-h LPS treatment; subsequent addition of WCE resulted in dose-dependent reduction of LPS-stimulated NO production (Fig. 2A). We subsequently examined the effects of WCE on PGE2 production in LPS-stimulated RAW 264.7 cells. After RAW 264.7 cells were stimulated with LPS for 24 h, PGE2 levels increased in the culture medium were suppressed by subsequent addition of WCE (Fig. 2B).


Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-κB-signaling Pathway.

Kim B, Kim JE, Choi BK, Kim HS - Biomol Ther (Seoul) (2015)

Effects of WCE on LPS-induced NO and PGE2 production, and iNOS expression. (A) RAW 264.7 cells were treated with 1 μg/mL of LPS alone or in combination with varying concentrations of WCE for a nitrite assay. The extracellular medium containing nitrate was analyzed by a Griess regent system. (B) The PGE2 concentration in the supernatants was determined by ELISA *p<0.05, **p<0.01 compared to the LPS treated group. Values are presented as means ± standard error of the mean (SEM). (C) Western blot analysis of iNOS. β-actin was used as an internal standard. (D) RT-PCR analysis of iNOS. β-actin was used as an internal standard. The PCR products were resolved on a 2% agarose gel.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4286755&req=5

f2-bt-23-90: Effects of WCE on LPS-induced NO and PGE2 production, and iNOS expression. (A) RAW 264.7 cells were treated with 1 μg/mL of LPS alone or in combination with varying concentrations of WCE for a nitrite assay. The extracellular medium containing nitrate was analyzed by a Griess regent system. (B) The PGE2 concentration in the supernatants was determined by ELISA *p<0.05, **p<0.01 compared to the LPS treated group. Values are presented as means ± standard error of the mean (SEM). (C) Western blot analysis of iNOS. β-actin was used as an internal standard. (D) RT-PCR analysis of iNOS. β-actin was used as an internal standard. The PCR products were resolved on a 2% agarose gel.
Mentions: Cell viability was not affected by 100 μg/mL WCE (data not shown). Therefore, all subsequent experiments on inflammatory mediator production were conducted with 100 μg/mL WCE. To investigate the anti-inflammatory effects of WCE, we examined its effect on NO production in LPS-induced macrophages. NO production greatly increased after 24-h LPS treatment; subsequent addition of WCE resulted in dose-dependent reduction of LPS-stimulated NO production (Fig. 2A). We subsequently examined the effects of WCE on PGE2 production in LPS-stimulated RAW 264.7 cells. After RAW 264.7 cells were stimulated with LPS for 24 h, PGE2 levels increased in the culture medium were suppressed by subsequent addition of WCE (Fig. 2B).

Bottom Line: In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species.The cytokine array results showed that WCE inhibited inflammatory cytokine secretion.These results indicate that WCE may be a promising topical anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Skin & Bio Research, Ellead Co., Ltd., Seongnam 463-824.

ABSTRACT
Water chestnut (Trapa japonica Flerov.) is an annual aquatic plant. In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species. In addition, water chestnut extract (WCE) inhibited lipopolysaccharide (LPS)-induced nitric oxide production and suppressed mRNA and protein expression of the inducible nitric oxide synthase gene. The cytokine array results showed that WCE inhibited inflammatory cytokine secretion. Also, WCE reduced tumor necrosis factor-α-and interleukin-6-induced nuclear factor-αB activity. Furthermore, during sodium lauryl sulfate (SLS)-induced irritation of human skin, WCE reduced SLS-induced skin erythema and improved barrier regeneration. These results indicate that WCE may be a promising topical anti-inflammatory agent.

No MeSH data available.


Related in: MedlinePlus