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Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-κB-signaling Pathway.

Kim B, Kim JE, Choi BK, Kim HS - Biomol Ther (Seoul) (2015)

Bottom Line: In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species.The cytokine array results showed that WCE inhibited inflammatory cytokine secretion.These results indicate that WCE may be a promising topical anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Skin & Bio Research, Ellead Co., Ltd., Seongnam 463-824.

ABSTRACT
Water chestnut (Trapa japonica Flerov.) is an annual aquatic plant. In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species. In addition, water chestnut extract (WCE) inhibited lipopolysaccharide (LPS)-induced nitric oxide production and suppressed mRNA and protein expression of the inducible nitric oxide synthase gene. The cytokine array results showed that WCE inhibited inflammatory cytokine secretion. Also, WCE reduced tumor necrosis factor-α-and interleukin-6-induced nuclear factor-αB activity. Furthermore, during sodium lauryl sulfate (SLS)-induced irritation of human skin, WCE reduced SLS-induced skin erythema and improved barrier regeneration. These results indicate that WCE may be a promising topical anti-inflammatory agent.

No MeSH data available.


Related in: MedlinePlus

Effect of WCE on DPPH radical-scavenging activity and intracellular ROS accumulation induced by H2O2. (A) HaCat cells were treated with WCE or vitamin C (Vit. C). and then DPPH radical-scavenging activities were estimated, black bar WCE treated group, white bar vitamin C treated group. ***p<0.001 compared to the untreated control group. §p<0.05 compared to the vitamin C treated group. (B) Ha-Cat cells were treated with DCF-DA in the presence or absence of WCE for 2 h, followed by H2O2 treatment, black bar WCE treated group, white bar H2O2 treated group. **p< 0.01 compared to H2O2 treated group. Values are presented as means ± standard error of the mean (SEM).
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f1-bt-23-90: Effect of WCE on DPPH radical-scavenging activity and intracellular ROS accumulation induced by H2O2. (A) HaCat cells were treated with WCE or vitamin C (Vit. C). and then DPPH radical-scavenging activities were estimated, black bar WCE treated group, white bar vitamin C treated group. ***p<0.001 compared to the untreated control group. §p<0.05 compared to the vitamin C treated group. (B) Ha-Cat cells were treated with DCF-DA in the presence or absence of WCE for 2 h, followed by H2O2 treatment, black bar WCE treated group, white bar H2O2 treated group. **p< 0.01 compared to H2O2 treated group. Values are presented as means ± standard error of the mean (SEM).

Mentions: To determine the anti-oxidant capacity of WCE, its antioxidant activity was determined by measuring the DPPH radicalscavenging reaction. Compared to treatment of the cells with the vitamin C positive control, treatment of HaCat cells with WCE markedly increased DPPH radical-scavenging activity according to concentration (Fig. 1A). WCE exhibited highly radical-scavenging activity (SC50=12.8 μg/mL; vitamin C, SC50=2.1 μg/mL). The intracellular ROS-scavenging activity of WCE was monitored by the dichlorofluorescein (DCF) fluorescence intensity of H2O2 induction. The WCE reduced intracellular ROS in a dose dependently manner (Fig. 1B). These results indicate that WCE has the anti-oxidant capacity.


Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-κB-signaling Pathway.

Kim B, Kim JE, Choi BK, Kim HS - Biomol Ther (Seoul) (2015)

Effect of WCE on DPPH radical-scavenging activity and intracellular ROS accumulation induced by H2O2. (A) HaCat cells were treated with WCE or vitamin C (Vit. C). and then DPPH radical-scavenging activities were estimated, black bar WCE treated group, white bar vitamin C treated group. ***p<0.001 compared to the untreated control group. §p<0.05 compared to the vitamin C treated group. (B) Ha-Cat cells were treated with DCF-DA in the presence or absence of WCE for 2 h, followed by H2O2 treatment, black bar WCE treated group, white bar H2O2 treated group. **p< 0.01 compared to H2O2 treated group. Values are presented as means ± standard error of the mean (SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4286755&req=5

f1-bt-23-90: Effect of WCE on DPPH radical-scavenging activity and intracellular ROS accumulation induced by H2O2. (A) HaCat cells were treated with WCE or vitamin C (Vit. C). and then DPPH radical-scavenging activities were estimated, black bar WCE treated group, white bar vitamin C treated group. ***p<0.001 compared to the untreated control group. §p<0.05 compared to the vitamin C treated group. (B) Ha-Cat cells were treated with DCF-DA in the presence or absence of WCE for 2 h, followed by H2O2 treatment, black bar WCE treated group, white bar H2O2 treated group. **p< 0.01 compared to H2O2 treated group. Values are presented as means ± standard error of the mean (SEM).
Mentions: To determine the anti-oxidant capacity of WCE, its antioxidant activity was determined by measuring the DPPH radicalscavenging reaction. Compared to treatment of the cells with the vitamin C positive control, treatment of HaCat cells with WCE markedly increased DPPH radical-scavenging activity according to concentration (Fig. 1A). WCE exhibited highly radical-scavenging activity (SC50=12.8 μg/mL; vitamin C, SC50=2.1 μg/mL). The intracellular ROS-scavenging activity of WCE was monitored by the dichlorofluorescein (DCF) fluorescence intensity of H2O2 induction. The WCE reduced intracellular ROS in a dose dependently manner (Fig. 1B). These results indicate that WCE has the anti-oxidant capacity.

Bottom Line: In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species.The cytokine array results showed that WCE inhibited inflammatory cytokine secretion.These results indicate that WCE may be a promising topical anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Skin & Bio Research, Ellead Co., Ltd., Seongnam 463-824.

ABSTRACT
Water chestnut (Trapa japonica Flerov.) is an annual aquatic plant. In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species. In addition, water chestnut extract (WCE) inhibited lipopolysaccharide (LPS)-induced nitric oxide production and suppressed mRNA and protein expression of the inducible nitric oxide synthase gene. The cytokine array results showed that WCE inhibited inflammatory cytokine secretion. Also, WCE reduced tumor necrosis factor-α-and interleukin-6-induced nuclear factor-αB activity. Furthermore, during sodium lauryl sulfate (SLS)-induced irritation of human skin, WCE reduced SLS-induced skin erythema and improved barrier regeneration. These results indicate that WCE may be a promising topical anti-inflammatory agent.

No MeSH data available.


Related in: MedlinePlus