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Therapeutic effects of s-petasin on disease models of asthma and peritonitis.

Lee KP, Kang S, Noh MS, Park SJ, Kim JM, Chung HY, Je NK, Lee YG, Choi YW, Im DS - Biomol Ther (Seoul) (2015)

Bottom Line: S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca(2+) increase in RBL-2H3 mast cells.Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages.This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

View Article: PubMed Central - PubMed

Affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735.

ABSTRACT
To explore the anti-allergic and anti-inflammatory effects of extracts of Petasites genus, we studied the effects of s-petasin, a major sesquiterpene from Petasites formosanus (a butterbur species) on asthma and peritonitis models. In an ovalbumin-induced mouse asthma model, s-petasin significantly inhibited the accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar fluids. S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca(2+) increase in RBL-2H3 mast cells. S-petasin inhibited the LPS induction of iNOS at the RNA and protein levels in mouse peritoneal macrophages. Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages. Furthermore, in an LPS-induced mouse model of peritonitis, s-petasin significantly inhibited the accumulation of polymorpho nuclear and mononuclear leukocytes in peritoneal cavity. This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

No MeSH data available.


Related in: MedlinePlus

S-petasin inhibited the productions of NO and prostaglandin E2 in mouse peritoneal macrophages. Mouse peritoneal macrophages were treated with s-petasin and 1 h later stimulated with 100 ng/ml LPS. LPS-induced productions of nitrite and PGE2 were measured. S-petasin was found to dose-dependently inhibit nitrite production (A) and PGE2 production (B). Results are the means ± SEs of three independent experiments in triplicate. Statistical significance: *p<0.05, **p<0.01, ***p<0.001 vs. LPS-treated group.
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f7-bt-23-45: S-petasin inhibited the productions of NO and prostaglandin E2 in mouse peritoneal macrophages. Mouse peritoneal macrophages were treated with s-petasin and 1 h later stimulated with 100 ng/ml LPS. LPS-induced productions of nitrite and PGE2 were measured. S-petasin was found to dose-dependently inhibit nitrite production (A) and PGE2 production (B). Results are the means ± SEs of three independent experiments in triplicate. Statistical significance: *p<0.05, **p<0.01, ***p<0.001 vs. LPS-treated group.

Mentions: To confirm the inhibitory effects of s-petasin on the inductions of iNOS and COX-2, concentrations of their enzyme products, that is, nitric oxide (NO) and prostaglandin E2 (PGE2), were measured. As shown in Fig. 7, LPS (100 ng/ ml) induced the productions of NO and PGE2, and s-petasin treatment significantly and concentration-dependently inhibited NO production (Fig. 7A). S-petasin showed concentrationdependent inhibition of PGE2 production but only statistically significant at 10 μM of s-petasin (Fig. 7B), which is consistent to the effects of s-petasin on mRNA and protein expressions of COX-2 (Fig. 5, 6).


Therapeutic effects of s-petasin on disease models of asthma and peritonitis.

Lee KP, Kang S, Noh MS, Park SJ, Kim JM, Chung HY, Je NK, Lee YG, Choi YW, Im DS - Biomol Ther (Seoul) (2015)

S-petasin inhibited the productions of NO and prostaglandin E2 in mouse peritoneal macrophages. Mouse peritoneal macrophages were treated with s-petasin and 1 h later stimulated with 100 ng/ml LPS. LPS-induced productions of nitrite and PGE2 were measured. S-petasin was found to dose-dependently inhibit nitrite production (A) and PGE2 production (B). Results are the means ± SEs of three independent experiments in triplicate. Statistical significance: *p<0.05, **p<0.01, ***p<0.001 vs. LPS-treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4286749&req=5

f7-bt-23-45: S-petasin inhibited the productions of NO and prostaglandin E2 in mouse peritoneal macrophages. Mouse peritoneal macrophages were treated with s-petasin and 1 h later stimulated with 100 ng/ml LPS. LPS-induced productions of nitrite and PGE2 were measured. S-petasin was found to dose-dependently inhibit nitrite production (A) and PGE2 production (B). Results are the means ± SEs of three independent experiments in triplicate. Statistical significance: *p<0.05, **p<0.01, ***p<0.001 vs. LPS-treated group.
Mentions: To confirm the inhibitory effects of s-petasin on the inductions of iNOS and COX-2, concentrations of their enzyme products, that is, nitric oxide (NO) and prostaglandin E2 (PGE2), were measured. As shown in Fig. 7, LPS (100 ng/ ml) induced the productions of NO and PGE2, and s-petasin treatment significantly and concentration-dependently inhibited NO production (Fig. 7A). S-petasin showed concentrationdependent inhibition of PGE2 production but only statistically significant at 10 μM of s-petasin (Fig. 7B), which is consistent to the effects of s-petasin on mRNA and protein expressions of COX-2 (Fig. 5, 6).

Bottom Line: S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca(2+) increase in RBL-2H3 mast cells.Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages.This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

View Article: PubMed Central - PubMed

Affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735.

ABSTRACT
To explore the anti-allergic and anti-inflammatory effects of extracts of Petasites genus, we studied the effects of s-petasin, a major sesquiterpene from Petasites formosanus (a butterbur species) on asthma and peritonitis models. In an ovalbumin-induced mouse asthma model, s-petasin significantly inhibited the accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar fluids. S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca(2+) increase in RBL-2H3 mast cells. S-petasin inhibited the LPS induction of iNOS at the RNA and protein levels in mouse peritoneal macrophages. Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages. Furthermore, in an LPS-induced mouse model of peritonitis, s-petasin significantly inhibited the accumulation of polymorpho nuclear and mononuclear leukocytes in peritoneal cavity. This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

No MeSH data available.


Related in: MedlinePlus