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Therapeutic effects of s-petasin on disease models of asthma and peritonitis.

Lee KP, Kang S, Noh MS, Park SJ, Kim JM, Chung HY, Je NK, Lee YG, Choi YW, Im DS - Biomol Ther (Seoul) (2015)

Bottom Line: S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca(2+) increase in RBL-2H3 mast cells.Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages.This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

View Article: PubMed Central - PubMed

Affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735.

ABSTRACT
To explore the anti-allergic and anti-inflammatory effects of extracts of Petasites genus, we studied the effects of s-petasin, a major sesquiterpene from Petasites formosanus (a butterbur species) on asthma and peritonitis models. In an ovalbumin-induced mouse asthma model, s-petasin significantly inhibited the accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar fluids. S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca(2+) increase in RBL-2H3 mast cells. S-petasin inhibited the LPS induction of iNOS at the RNA and protein levels in mouse peritoneal macrophages. Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages. Furthermore, in an LPS-induced mouse model of peritonitis, s-petasin significantly inhibited the accumulation of polymorpho nuclear and mononuclear leukocytes in peritoneal cavity. This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

No MeSH data available.


Related in: MedlinePlus

S-petasin inhibited antigen-induced β-hexosamidase release from RBL-2H3 mast cells in a concentration-dependent manner. RBL-2H3 cells sensitized overnight with anti DNP-IgE were treated with different concentrations of s-petasin, and 30 minutes later challenged with DNP human serum albumin. Antigen-induced degranulation was determined by measuring the amount of released β-hexosamidase activity. S-petasin inhibited antigen-induced degranulation in RBL-2H3 mast cells in a dose-dependent manner. Results are the means ± SEs of three independent experiments.
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f3-bt-23-45: S-petasin inhibited antigen-induced β-hexosamidase release from RBL-2H3 mast cells in a concentration-dependent manner. RBL-2H3 cells sensitized overnight with anti DNP-IgE were treated with different concentrations of s-petasin, and 30 minutes later challenged with DNP human serum albumin. Antigen-induced degranulation was determined by measuring the amount of released β-hexosamidase activity. S-petasin inhibited antigen-induced degranulation in RBL-2H3 mast cells in a dose-dependent manner. Results are the means ± SEs of three independent experiments.

Mentions: Next, to confirm the in vivo anti-asthma effect of s-petasin and find underlining mechanisms for the effect, we utilized rat RBL-2H3 mast cells. Because degranulation of histamine, leukotrienes, and prostaglandins from antigen-exposed mast cells is a key step in allergic response, we investigated whether s-petasin inhibits antigen-induced degranulation in rat RBL-2H3 cells. Degranulation was measured by assessing β-hexosamidase activity in media after antigen exposure, as previously described (Dearman et al., 2005; Lu et al., 2012). Antigen-induced β-hexosamidase release was found to be inhibited concentration-dependently by s-petasin (Fig. 3). IC50 value was about 1 nM.


Therapeutic effects of s-petasin on disease models of asthma and peritonitis.

Lee KP, Kang S, Noh MS, Park SJ, Kim JM, Chung HY, Je NK, Lee YG, Choi YW, Im DS - Biomol Ther (Seoul) (2015)

S-petasin inhibited antigen-induced β-hexosamidase release from RBL-2H3 mast cells in a concentration-dependent manner. RBL-2H3 cells sensitized overnight with anti DNP-IgE were treated with different concentrations of s-petasin, and 30 minutes later challenged with DNP human serum albumin. Antigen-induced degranulation was determined by measuring the amount of released β-hexosamidase activity. S-petasin inhibited antigen-induced degranulation in RBL-2H3 mast cells in a dose-dependent manner. Results are the means ± SEs of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4286749&req=5

f3-bt-23-45: S-petasin inhibited antigen-induced β-hexosamidase release from RBL-2H3 mast cells in a concentration-dependent manner. RBL-2H3 cells sensitized overnight with anti DNP-IgE were treated with different concentrations of s-petasin, and 30 minutes later challenged with DNP human serum albumin. Antigen-induced degranulation was determined by measuring the amount of released β-hexosamidase activity. S-petasin inhibited antigen-induced degranulation in RBL-2H3 mast cells in a dose-dependent manner. Results are the means ± SEs of three independent experiments.
Mentions: Next, to confirm the in vivo anti-asthma effect of s-petasin and find underlining mechanisms for the effect, we utilized rat RBL-2H3 mast cells. Because degranulation of histamine, leukotrienes, and prostaglandins from antigen-exposed mast cells is a key step in allergic response, we investigated whether s-petasin inhibits antigen-induced degranulation in rat RBL-2H3 cells. Degranulation was measured by assessing β-hexosamidase activity in media after antigen exposure, as previously described (Dearman et al., 2005; Lu et al., 2012). Antigen-induced β-hexosamidase release was found to be inhibited concentration-dependently by s-petasin (Fig. 3). IC50 value was about 1 nM.

Bottom Line: S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca(2+) increase in RBL-2H3 mast cells.Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages.This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

View Article: PubMed Central - PubMed

Affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 609-735.

ABSTRACT
To explore the anti-allergic and anti-inflammatory effects of extracts of Petasites genus, we studied the effects of s-petasin, a major sesquiterpene from Petasites formosanus (a butterbur species) on asthma and peritonitis models. In an ovalbumin-induced mouse asthma model, s-petasin significantly inhibited the accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar fluids. S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca(2+) increase in RBL-2H3 mast cells. S-petasin inhibited the LPS induction of iNOS at the RNA and protein levels in mouse peritoneal macrophages. Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages. Furthermore, in an LPS-induced mouse model of peritonitis, s-petasin significantly inhibited the accumulation of polymorpho nuclear and mononuclear leukocytes in peritoneal cavity. This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

No MeSH data available.


Related in: MedlinePlus