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Histological investigation of the effect of soybean (Glycine max) extracts on the collagen layer and estrogen receptors in the skin of female rats.

Uyar B, Sivrikoz ON, Ozdemir U, Dasbasi T, Sacar H - Clinics (Sao Paulo) (2014)

Bottom Line: The groups comprised carboxymethyl cellulose-free control, carboxymethyl cellulose-plus control, 100-mg/kg n-hexane extract, 200-mg/kg n-hexane extract, 100-mg/kg ethyl acetate extract, 200-mg/kg ethyl acetate extract, 100-mg/kg ethanol extract and 200-mg/kg ethanol extract groups.Regarding the thickness of the collagen layer, only the 200-mg/kg ethyl acetate extract-treated group showed a significant increase compared with the control groups (p<0.05).Our data suggest that oral intake of three different total soybean extracts might have positive estrogenic effects on the skin and that only a high-dose ethyl acetate extract can increase the expression of collagen, which may prove to be beneficial for postmenopausal facial skin.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Faculty of Medicine, Sifa University, Izmir, Turkey.

ABSTRACT

Objectives: The purpose of this study was to analyze the effects of soybean extracts obtained using different extraction methods on the skin of female rats.

Method: A total of 64 female Sprague-Dawley rats were divided into 8 equal groups. Various extracts were administered to the female rats by oral gavage for one month. The groups comprised carboxymethyl cellulose-free control, carboxymethyl cellulose-plus control, 100-mg/kg n-hexane extract, 200-mg/kg n-hexane extract, 100-mg/kg ethyl acetate extract, 200-mg/kg ethyl acetate extract, 100-mg/kg ethanol extract and 200-mg/kg ethanol extract groups. The thickness of the collagen layer and the number of estrogen receptor-positive cells were evaluated.

Results: All the extract-treated groups showed a statistically significant decrease in the number of estrogen receptor-positive cells compared with the control groups. Regarding the thickness of the collagen layer, only the 200-mg/kg ethyl acetate extract-treated group showed a significant increase compared with the control groups (p<0.05).

Conclusions: Our data suggest that oral intake of three different total soybean extracts might have positive estrogenic effects on the skin and that only a high-dose ethyl acetate extract can increase the expression of collagen, which may prove to be beneficial for postmenopausal facial skin.

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Related in: MedlinePlus

Immunohistochemical staining of the ER (estrogen receptor) in skin biopsies from the CMC-free group (A), the 0.5% CMC-plus group (B), the group treated with the n-hexane extract at a dose of 100 mg/kg (C), the group treated with the n-hexane extract at a dose of 200 mg/kg (D), the group treated with the ethyl acetate extract at a dose of 100 mg/kg (E), the group treated with the ethyl acetate extract at a dose of 200 mg/kg (F), the group treated with the ethanol extract at a dose of 100 mg/kg (G), and the group treated with the ethanol extract at a dose of 200 mg/kg (H) (magnification, 40X). The arrows indicate positively stained cells in the dermal layer.
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f4-cln_69p854: Immunohistochemical staining of the ER (estrogen receptor) in skin biopsies from the CMC-free group (A), the 0.5% CMC-plus group (B), the group treated with the n-hexane extract at a dose of 100 mg/kg (C), the group treated with the n-hexane extract at a dose of 200 mg/kg (D), the group treated with the ethyl acetate extract at a dose of 100 mg/kg (E), the group treated with the ethyl acetate extract at a dose of 200 mg/kg (F), the group treated with the ethanol extract at a dose of 100 mg/kg (G), and the group treated with the ethanol extract at a dose of 200 mg/kg (H) (magnification, 40X). The arrows indicate positively stained cells in the dermal layer.

Mentions: There were no statistically significant differences between group E and group F and group H (FigureĀ 4). We did not observe any correlation between the collagen layer thickness and the number of estrogen receptor-positive cells.


Histological investigation of the effect of soybean (Glycine max) extracts on the collagen layer and estrogen receptors in the skin of female rats.

Uyar B, Sivrikoz ON, Ozdemir U, Dasbasi T, Sacar H - Clinics (Sao Paulo) (2014)

Immunohistochemical staining of the ER (estrogen receptor) in skin biopsies from the CMC-free group (A), the 0.5% CMC-plus group (B), the group treated with the n-hexane extract at a dose of 100 mg/kg (C), the group treated with the n-hexane extract at a dose of 200 mg/kg (D), the group treated with the ethyl acetate extract at a dose of 100 mg/kg (E), the group treated with the ethyl acetate extract at a dose of 200 mg/kg (F), the group treated with the ethanol extract at a dose of 100 mg/kg (G), and the group treated with the ethanol extract at a dose of 200 mg/kg (H) (magnification, 40X). The arrows indicate positively stained cells in the dermal layer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4286672&req=5

f4-cln_69p854: Immunohistochemical staining of the ER (estrogen receptor) in skin biopsies from the CMC-free group (A), the 0.5% CMC-plus group (B), the group treated with the n-hexane extract at a dose of 100 mg/kg (C), the group treated with the n-hexane extract at a dose of 200 mg/kg (D), the group treated with the ethyl acetate extract at a dose of 100 mg/kg (E), the group treated with the ethyl acetate extract at a dose of 200 mg/kg (F), the group treated with the ethanol extract at a dose of 100 mg/kg (G), and the group treated with the ethanol extract at a dose of 200 mg/kg (H) (magnification, 40X). The arrows indicate positively stained cells in the dermal layer.
Mentions: There were no statistically significant differences between group E and group F and group H (FigureĀ 4). We did not observe any correlation between the collagen layer thickness and the number of estrogen receptor-positive cells.

Bottom Line: The groups comprised carboxymethyl cellulose-free control, carboxymethyl cellulose-plus control, 100-mg/kg n-hexane extract, 200-mg/kg n-hexane extract, 100-mg/kg ethyl acetate extract, 200-mg/kg ethyl acetate extract, 100-mg/kg ethanol extract and 200-mg/kg ethanol extract groups.Regarding the thickness of the collagen layer, only the 200-mg/kg ethyl acetate extract-treated group showed a significant increase compared with the control groups (p<0.05).Our data suggest that oral intake of three different total soybean extracts might have positive estrogenic effects on the skin and that only a high-dose ethyl acetate extract can increase the expression of collagen, which may prove to be beneficial for postmenopausal facial skin.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Faculty of Medicine, Sifa University, Izmir, Turkey.

ABSTRACT

Objectives: The purpose of this study was to analyze the effects of soybean extracts obtained using different extraction methods on the skin of female rats.

Method: A total of 64 female Sprague-Dawley rats were divided into 8 equal groups. Various extracts were administered to the female rats by oral gavage for one month. The groups comprised carboxymethyl cellulose-free control, carboxymethyl cellulose-plus control, 100-mg/kg n-hexane extract, 200-mg/kg n-hexane extract, 100-mg/kg ethyl acetate extract, 200-mg/kg ethyl acetate extract, 100-mg/kg ethanol extract and 200-mg/kg ethanol extract groups. The thickness of the collagen layer and the number of estrogen receptor-positive cells were evaluated.

Results: All the extract-treated groups showed a statistically significant decrease in the number of estrogen receptor-positive cells compared with the control groups. Regarding the thickness of the collagen layer, only the 200-mg/kg ethyl acetate extract-treated group showed a significant increase compared with the control groups (p<0.05).

Conclusions: Our data suggest that oral intake of three different total soybean extracts might have positive estrogenic effects on the skin and that only a high-dose ethyl acetate extract can increase the expression of collagen, which may prove to be beneficial for postmenopausal facial skin.

Show MeSH
Related in: MedlinePlus