Plant defence responses in oilseed rape MINELESS plants after attack by the cabbage moth Mamestra brassicae.
Bottom Line: No-choice feeding experiments showed that M. brassicae larvae gained less weight and showed stunted growth when feeding on MINELESS plants compared to feeding on wild-type plants.M. brassicae feeding didn't affect myrosinase activity in MINELESS plants, but did reduce it in wild-type seedlings.Taken together, the outcomes are very interesting in terms of analysing the role of myrosin cells and the glucosinolate-myrosinase defence system in response to a generalist cabbage moth, suggesting that similar studies with other generalist or specialist insect herbivores, including above- and below-ground herbivores, would be useful.
Affiliation: Department of Biology, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway.Show MeSH
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Mentions: The feeding experiments with M. brassicae showed that larvae gained significantly less weight on MINELESS seedlings compared to the wild-type seedlings at all time points: (Experiment I) day 3, 8, 10, and 12, P < 0.001; (Experiment II) day 7 and 12, P < 0.001 (Fig. 1). The average weights of larvae feeding on MINELESS plants were 1.5, 2.2, 4.1, and 3.6 times lower than the average weights of larvae that had been feeding on the wild-type for day 3, 8, 10, and 12, respectively (Experiment I) (Fig. 1A). Similarly, in feeding Experiment II, the average larvae weights were observed to be 2.2 and 2.6 times lower when feeding MINELESS seedlings compared to the wild-type for day 7 and 12, respectively (Fig. 1B). Both experiments showed similar reduction (2.2 times) in larvae weights for day 7 (Experiment II) and day 8 (Experiment I). How larvae feed on wild-type and MINELESS plants, and how they appeared after 12 days of feeding, can be seen in Supplementary Videos V1 and V2.
Affiliation: Department of Biology, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway.