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Study of methanogen communities associated with different rumen protozoal populations.

Belanche A, de la Fuente G, Newbold CJ - FEMS Microbiol. Ecol. (2014)

Bottom Line: Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis.On the contrary, PAM constituted a more variable community (48% similarity), which differed between holotrich and total protozoa (P < 0.001).Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.

No MeSH data available.


Diagram depicting sheep inoculation and rumen sampling.
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fig01: Diagram depicting sheep inoculation and rumen sampling.

Mentions: Four mature Texel-cross-breed sheep were used as rumen fluid donors in two consecutive 3-month periods (holotrich-monofaunated and total-faunated sheep). Lambs were isolated from their mothers within 24 h after lambing and maintained protozoa-free by avoiding protozoal transmission from adult ruminants. When lambs became adults (5 years old and 94 ± 8.6 kg average body weight) and after checking their protozoa-free status by ruminal microscopy, animals were then orally inoculated with a mixed holotrich population composed by isolates of Isotricha prostoma, I. intestinalis and Dasytricha ruminantium. These protozoal isolates were defrosted at 39 °C, diluted in simplex type salts solution (STS) (Williams & Coleman, 1992) and orally inoculated in all sheep to generate holotrich-monofaunated sheep in period 1. For period 2, the same four animals were orally inoculated with pooled rumen fluid obtained from four control animals (with a natural protozoal population). As a result, experimental sheep became totally faunated in period 2 (Fig.1).


Study of methanogen communities associated with different rumen protozoal populations.

Belanche A, de la Fuente G, Newbold CJ - FEMS Microbiol. Ecol. (2014)

Diagram depicting sheep inoculation and rumen sampling.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4286163&req=5

fig01: Diagram depicting sheep inoculation and rumen sampling.
Mentions: Four mature Texel-cross-breed sheep were used as rumen fluid donors in two consecutive 3-month periods (holotrich-monofaunated and total-faunated sheep). Lambs were isolated from their mothers within 24 h after lambing and maintained protozoa-free by avoiding protozoal transmission from adult ruminants. When lambs became adults (5 years old and 94 ± 8.6 kg average body weight) and after checking their protozoa-free status by ruminal microscopy, animals were then orally inoculated with a mixed holotrich population composed by isolates of Isotricha prostoma, I. intestinalis and Dasytricha ruminantium. These protozoal isolates were defrosted at 39 °C, diluted in simplex type salts solution (STS) (Williams & Coleman, 1992) and orally inoculated in all sheep to generate holotrich-monofaunated sheep in period 1. For period 2, the same four animals were orally inoculated with pooled rumen fluid obtained from four control animals (with a natural protozoal population). As a result, experimental sheep became totally faunated in period 2 (Fig.1).

Bottom Line: Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis.On the contrary, PAM constituted a more variable community (48% similarity), which differed between holotrich and total protozoa (P < 0.001).Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.

No MeSH data available.