Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid Crambe abyssinica.
Bottom Line: Crambe abyssinica is an alternative promising producer of this acid as it has 55%-60% of erucic acid in its oil.In this study, we further investigated different aspects of oil biosynthesis in the developing GM Crambe seeds in comparison with wild-type (Wt) Crambe, rapeseed and safflower (Carthamus tinctorius).Likely bottlenecks in the accumulation of erucic acid during early stages of GM Crambe seed development are discussed.
Affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.Show MeSH
Mentions: To correlate the detected in vitro activities of the flow of glycerol backbone towards TAGs in the microsomal membranes of the developing seeds with in vivo activities, we fed [14C]glycerol to detached developing embryos of rapeseed and Wt Crambe collected 20 DAF and determined the amount of radioactivity in the complex lipid at different time intervals. Only four lipids, PA, DAG, PC and TAG, contained a significant amount of radioactivity, of which PA accounted only for a very small portion of the total labelled lipids (Figure7). DAG and TAG were the main labelled lipids at all the time points in Crambe seeds, while the radioactivity in PC was two to three times less than DAG or TAG. In rapeseed, DAG was the highest labelled lipid, while TAG showed about half of the radioactivity compared with PC.
Affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.