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Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid Crambe abyssinica.

Guan R, Lager I, Li X, Stymne S, Zhu LH - Plant Biotechnol. J. (2013)

Bottom Line: Crambe abyssinica is an alternative promising producer of this acid as it has 55%-60% of erucic acid in its oil.In this study, we further investigated different aspects of oil biosynthesis in the developing GM Crambe seeds in comparison with wild-type (Wt) Crambe, rapeseed and safflower (Carthamus tinctorius).Likely bottlenecks in the accumulation of erucic acid during early stages of GM Crambe seed development are discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.

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Time course incorporation of radioactivity into various lipids in microsomal preparations from developing seeds (from species indicated in the figure) incubated with glycerol-labelled [14C]18:1-LPA and nonradioactive 22:1-CoA. Wt, wild type; GM, genetically modified; LPA, lysophosphatidic acid; MAG, monoacylglycerol; PA, phosphatidic acid; DAG, diacylglycerol; PC, phosphatidylcholine; TAG, triacylglycerols. Results are shown from triplicate samples ± SD.
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fig05: Time course incorporation of radioactivity into various lipids in microsomal preparations from developing seeds (from species indicated in the figure) incubated with glycerol-labelled [14C]18:1-LPA and nonradioactive 22:1-CoA. Wt, wild type; GM, genetically modified; LPA, lysophosphatidic acid; MAG, monoacylglycerol; PA, phosphatidic acid; DAG, diacylglycerol; PC, phosphatidylcholine; TAG, triacylglycerols. Results are shown from triplicate samples ± SD.

Mentions: Incubations with glycerol-labelled [14C]18:1-LPA and nonradioactive 22:1-CoA demonstrated a dramatic difference in the metabolism between GM and Wt Crambe (Figure5). Virtually, all LPA was acylated with 22:1 within 20 min with the membranes of GM Crambe, further confirming the functional role of introduced Limnanthes LPAAT gene in the acylation of 22:1 at the sn-2 position. In contrast, very little labelled PA was detected in Wt Crambe where a substantial amount of labelled MAG was formed. The rapeseed had somewhat better ability to acylate 22:1 than the Wt Crambe. Surprisingly, a substantial amount of 22:1 was acylated to 18:1-LPA in safflower microsomes and PA was efficiently converted to DAG and further to PC and TAG, again demonstrating no constrains in DAG-PC interconversion due to erucoyl groups in DAG in these microsomes.


Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid Crambe abyssinica.

Guan R, Lager I, Li X, Stymne S, Zhu LH - Plant Biotechnol. J. (2013)

Time course incorporation of radioactivity into various lipids in microsomal preparations from developing seeds (from species indicated in the figure) incubated with glycerol-labelled [14C]18:1-LPA and nonradioactive 22:1-CoA. Wt, wild type; GM, genetically modified; LPA, lysophosphatidic acid; MAG, monoacylglycerol; PA, phosphatidic acid; DAG, diacylglycerol; PC, phosphatidylcholine; TAG, triacylglycerols. Results are shown from triplicate samples ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4286110&req=5

fig05: Time course incorporation of radioactivity into various lipids in microsomal preparations from developing seeds (from species indicated in the figure) incubated with glycerol-labelled [14C]18:1-LPA and nonradioactive 22:1-CoA. Wt, wild type; GM, genetically modified; LPA, lysophosphatidic acid; MAG, monoacylglycerol; PA, phosphatidic acid; DAG, diacylglycerol; PC, phosphatidylcholine; TAG, triacylglycerols. Results are shown from triplicate samples ± SD.
Mentions: Incubations with glycerol-labelled [14C]18:1-LPA and nonradioactive 22:1-CoA demonstrated a dramatic difference in the metabolism between GM and Wt Crambe (Figure5). Virtually, all LPA was acylated with 22:1 within 20 min with the membranes of GM Crambe, further confirming the functional role of introduced Limnanthes LPAAT gene in the acylation of 22:1 at the sn-2 position. In contrast, very little labelled PA was detected in Wt Crambe where a substantial amount of labelled MAG was formed. The rapeseed had somewhat better ability to acylate 22:1 than the Wt Crambe. Surprisingly, a substantial amount of 22:1 was acylated to 18:1-LPA in safflower microsomes and PA was efficiently converted to DAG and further to PC and TAG, again demonstrating no constrains in DAG-PC interconversion due to erucoyl groups in DAG in these microsomes.

Bottom Line: Crambe abyssinica is an alternative promising producer of this acid as it has 55%-60% of erucic acid in its oil.In this study, we further investigated different aspects of oil biosynthesis in the developing GM Crambe seeds in comparison with wild-type (Wt) Crambe, rapeseed and safflower (Carthamus tinctorius).Likely bottlenecks in the accumulation of erucic acid during early stages of GM Crambe seed development are discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.

Show MeSH