Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid Crambe abyssinica.
Bottom Line: Crambe abyssinica is an alternative promising producer of this acid as it has 55%-60% of erucic acid in its oil.In this study, we further investigated different aspects of oil biosynthesis in the developing GM Crambe seeds in comparison with wild-type (Wt) Crambe, rapeseed and safflower (Carthamus tinctorius).Likely bottlenecks in the accumulation of erucic acid during early stages of GM Crambe seed development are discussed.
Affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.Show MeSH
Mentions: Incubations with glycerol-labelled [14C]18:1-LPA and nonradioactive 22:1-CoA demonstrated a dramatic difference in the metabolism between GM and Wt Crambe (Figure5). Virtually, all LPA was acylated with 22:1 within 20 min with the membranes of GM Crambe, further confirming the functional role of introduced Limnanthes LPAAT gene in the acylation of 22:1 at the sn-2 position. In contrast, very little labelled PA was detected in Wt Crambe where a substantial amount of labelled MAG was formed. The rapeseed had somewhat better ability to acylate 22:1 than the Wt Crambe. Surprisingly, a substantial amount of 22:1 was acylated to 18:1-LPA in safflower microsomes and PA was efficiently converted to DAG and further to PC and TAG, again demonstrating no constrains in DAG-PC interconversion due to erucoyl groups in DAG in these microsomes.
Affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.