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Intricacies of redoxome function demonstrated with a simple in vitro chemiluminescence method, with special reference to vitamin B12 as antioxidant.

Bøyum A, Forstrøm RJ, Sefland I, Sand KL, Benestad HB - Scand. J. Immunol. (2014)

Bottom Line: The homeostatic control of the redox system (the redoxome) in mammalian cells depends upon a large number of interacting molecules, which tend to buffer the electronegativity of cells against oxidants or reductants.Simple dose-responses were found, as for the PMN proteins S100A9 (A9) and S100A8 (A8), and the system also revealed the reducing capacity of vitamin B12 (Cbl) and lutein.Biphasic responses were also recorded for some dose-response set-ups and are tentatively explained by a 'balance hypothesis' for the redoxome.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

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Biphasic dose–response of erythrocyte (RBC) extract and haemoglobin (Hb). RBC extract from two individuals (see Materials and methods) were compared (quadruplicate measurements) with corresponding concentrations of purified Hb (Sigma).
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fig04: Biphasic dose–response of erythrocyte (RBC) extract and haemoglobin (Hb). RBC extract from two individuals (see Materials and methods) were compared (quadruplicate measurements) with corresponding concentrations of purified Hb (Sigma).

Mentions: Chemiluminescence stimulation may also be obtained with erythrocyte lysate (Fig. 4). It is probably caused by its haemoglobin and was enhanced 2- to 3-fold by 20 μm NaOCl (not shown, P < 0.01). The chemiluminescence was much stronger with purified haemoglobin (Sigma) (Fig. 4), presumably due to an effect of methaemoglobin, and, noticeably, there was no additional effect of NaOCl.


Intricacies of redoxome function demonstrated with a simple in vitro chemiluminescence method, with special reference to vitamin B12 as antioxidant.

Bøyum A, Forstrøm RJ, Sefland I, Sand KL, Benestad HB - Scand. J. Immunol. (2014)

Biphasic dose–response of erythrocyte (RBC) extract and haemoglobin (Hb). RBC extract from two individuals (see Materials and methods) were compared (quadruplicate measurements) with corresponding concentrations of purified Hb (Sigma).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4285856&req=5

fig04: Biphasic dose–response of erythrocyte (RBC) extract and haemoglobin (Hb). RBC extract from two individuals (see Materials and methods) were compared (quadruplicate measurements) with corresponding concentrations of purified Hb (Sigma).
Mentions: Chemiluminescence stimulation may also be obtained with erythrocyte lysate (Fig. 4). It is probably caused by its haemoglobin and was enhanced 2- to 3-fold by 20 μm NaOCl (not shown, P < 0.01). The chemiluminescence was much stronger with purified haemoglobin (Sigma) (Fig. 4), presumably due to an effect of methaemoglobin, and, noticeably, there was no additional effect of NaOCl.

Bottom Line: The homeostatic control of the redox system (the redoxome) in mammalian cells depends upon a large number of interacting molecules, which tend to buffer the electronegativity of cells against oxidants or reductants.Simple dose-responses were found, as for the PMN proteins S100A9 (A9) and S100A8 (A8), and the system also revealed the reducing capacity of vitamin B12 (Cbl) and lutein.Biphasic responses were also recorded for some dose-response set-ups and are tentatively explained by a 'balance hypothesis' for the redoxome.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

Show MeSH