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The evolution of nanopore sequencing.

Wang Y, Yang Q, Wang Z - Front Genet (2015)

Bottom Line: Both of protein and solid-state nanopores have been extensively investigated for a series of issues, from detection of ionic current blockage to field-effect-transistor (FET) sensors.A newly released protein nanopore sequencer has shown encouraging potential that nanopore sequencing will ultimately fulfill the gold standards.In this review, we address advances, challenges, and possible solutions of nanopore sequencing according to these standards.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University Shanghai, China.

ABSTRACT
The "$1000 Genome" project has been drawing increasing attention since its launch a decade ago. Nanopore sequencing, the third-generation, is believed to be one of the most promising sequencing technologies to reach four gold standards set for the "$1000 Genome" while the second-generation sequencing technologies are bringing about a revolution in life sciences, particularly in genome sequencing-based personalized medicine. Both of protein and solid-state nanopores have been extensively investigated for a series of issues, from detection of ionic current blockage to field-effect-transistor (FET) sensors. A newly released protein nanopore sequencer has shown encouraging potential that nanopore sequencing will ultimately fulfill the gold standards. In this review, we address advances, challenges, and possible solutions of nanopore sequencing according to these standards.

No MeSH data available.


Related in: MedlinePlus

Nanopore structures of α-HL (A) and MspA (B) (Venkatesan and Bashir, 2011). Reproduced by copyright permission of Nature Publishing Group.
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Figure 1: Nanopore structures of α-HL (A) and MspA (B) (Venkatesan and Bashir, 2011). Reproduced by copyright permission of Nature Publishing Group.

Mentions: Bayley and colleagues reported that α-HL is a 232.4 kDa membrane channel protein (Gouaux et al., 1994). Their crystal structure analysis of α-HL revealed a ~10 nm-high hollow mushroom-shaped homoheptamer complex containing a ~10 nm-wide extramembranal cap and a ~5.2 nm-long transmembrane β-barrel stem (Song et al., 1996). The minimum diameter at the constriction site of the channel is ~1.4 nm, which is connected to the β-barrel with the vestibule of 2.6 nm in diameter at the trans side (Figure 1A).


The evolution of nanopore sequencing.

Wang Y, Yang Q, Wang Z - Front Genet (2015)

Nanopore structures of α-HL (A) and MspA (B) (Venkatesan and Bashir, 2011). Reproduced by copyright permission of Nature Publishing Group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4285804&req=5

Figure 1: Nanopore structures of α-HL (A) and MspA (B) (Venkatesan and Bashir, 2011). Reproduced by copyright permission of Nature Publishing Group.
Mentions: Bayley and colleagues reported that α-HL is a 232.4 kDa membrane channel protein (Gouaux et al., 1994). Their crystal structure analysis of α-HL revealed a ~10 nm-high hollow mushroom-shaped homoheptamer complex containing a ~10 nm-wide extramembranal cap and a ~5.2 nm-long transmembrane β-barrel stem (Song et al., 1996). The minimum diameter at the constriction site of the channel is ~1.4 nm, which is connected to the β-barrel with the vestibule of 2.6 nm in diameter at the trans side (Figure 1A).

Bottom Line: Both of protein and solid-state nanopores have been extensively investigated for a series of issues, from detection of ionic current blockage to field-effect-transistor (FET) sensors.A newly released protein nanopore sequencer has shown encouraging potential that nanopore sequencing will ultimately fulfill the gold standards.In this review, we address advances, challenges, and possible solutions of nanopore sequencing according to these standards.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University Shanghai, China.

ABSTRACT
The "$1000 Genome" project has been drawing increasing attention since its launch a decade ago. Nanopore sequencing, the third-generation, is believed to be one of the most promising sequencing technologies to reach four gold standards set for the "$1000 Genome" while the second-generation sequencing technologies are bringing about a revolution in life sciences, particularly in genome sequencing-based personalized medicine. Both of protein and solid-state nanopores have been extensively investigated for a series of issues, from detection of ionic current blockage to field-effect-transistor (FET) sensors. A newly released protein nanopore sequencer has shown encouraging potential that nanopore sequencing will ultimately fulfill the gold standards. In this review, we address advances, challenges, and possible solutions of nanopore sequencing according to these standards.

No MeSH data available.


Related in: MedlinePlus