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Evaluation of nystatin containing chitosan hydrogels as potential dual action bio-active restorative materials: in vitro approach.

Perchyonok VT, Reher V, Zhang S, Basson N, Grobler S - J Funct Biomater (2014)

Bottom Line: The physico-chemical features, including surface morphology (SEM), release behaviors, stability of the therapeutic agent-antioxidant-chitosan, were measured and compared to the earlier reported chitosan-antioxidant containing hydrogels.The bio-adhesive capacity of the materials in the in vitro system was tested and quantified.It was found that the favorable synergistic effect of free radical built-in defense mechanism of the new functional materials increased sustainable bio-adhesion and therefore acted as a functional multi-dimensional restorative material with potential application in wound healing in vitro.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Department, VTPCHEM PTY LTD., Southport 4215, Australia. tamaraperchyonok@gmail.com.

ABSTRACT
Healing is a specific biological process related to the general phenomenon of growth and tissue regeneration and is a process generally affected by several systemic conditions or as detrimental side-effects of chemotherapy- and radiotherapy-induced inflammation of the oral mucosa. The objectives of this study is to evaluate the novel chitosan based functional drug delivery systems, which can be successfully incorporated into "dual action bioactive restorative materials", capable of inducing in vitro improved wound healing prototype and containing an antibiotic, such as nystatin, krill oil as an antioxidant and hydroxyapatite as a molecular bone scaffold, which is naturally present in bone and is reported to be successfully used in promoting bone integration when implanted as well as promoting healing. The hydrogels were prepared using a protocol as previously reported by us. The physico-chemical features, including surface morphology (SEM), release behaviors, stability of the therapeutic agent-antioxidant-chitosan, were measured and compared to the earlier reported chitosan-antioxidant containing hydrogels. Structural investigations of the reactive surface of the hydrogel are reported. Release of nystatin was investigated for all newly prepared hydrogels. Bio-adhesive studies were performed in order to assess the suitability of these designer materials. Free radical defense capacity of the biomaterials was evaluated using established in vitro model. The bio-adhesive capacity of the materials in the in vitro system was tested and quantified. It was found that the favorable synergistic effect of free radical built-in defense mechanism of the new functional materials increased sustainable bio-adhesion and therefore acted as a functional multi-dimensional restorative material with potential application in wound healing in vitro.

No MeSH data available.


Related in: MedlinePlus

SEM photomicrograph of freeze-dried gels 1–5 (a–e).
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jfb-05-00259-f001: SEM photomicrograph of freeze-dried gels 1–5 (a–e).

Mentions: The SEM images were obtained to characterize the microstructure of the freeze-dried gels and are presented in Figure 1. It could be seen that the gels displayed a homogeneous pore structure similar to a sponge. SEM analysis revealed interconnected pores of different sizes and flat, relatively smooth walls. The biomaterial remained intact after 24 days of immersion in artificial saliva as was confirmed by SEM. It was thought that the micro-porous structure of the gels could lead to high internal surface areas with low diffusional resistance in the gels. The pH of the prepared gels ranged from 5.46 to 6.94 (Table 1).


Evaluation of nystatin containing chitosan hydrogels as potential dual action bio-active restorative materials: in vitro approach.

Perchyonok VT, Reher V, Zhang S, Basson N, Grobler S - J Funct Biomater (2014)

SEM photomicrograph of freeze-dried gels 1–5 (a–e).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4285406&req=5

jfb-05-00259-f001: SEM photomicrograph of freeze-dried gels 1–5 (a–e).
Mentions: The SEM images were obtained to characterize the microstructure of the freeze-dried gels and are presented in Figure 1. It could be seen that the gels displayed a homogeneous pore structure similar to a sponge. SEM analysis revealed interconnected pores of different sizes and flat, relatively smooth walls. The biomaterial remained intact after 24 days of immersion in artificial saliva as was confirmed by SEM. It was thought that the micro-porous structure of the gels could lead to high internal surface areas with low diffusional resistance in the gels. The pH of the prepared gels ranged from 5.46 to 6.94 (Table 1).

Bottom Line: The physico-chemical features, including surface morphology (SEM), release behaviors, stability of the therapeutic agent-antioxidant-chitosan, were measured and compared to the earlier reported chitosan-antioxidant containing hydrogels.The bio-adhesive capacity of the materials in the in vitro system was tested and quantified.It was found that the favorable synergistic effect of free radical built-in defense mechanism of the new functional materials increased sustainable bio-adhesion and therefore acted as a functional multi-dimensional restorative material with potential application in wound healing in vitro.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Department, VTPCHEM PTY LTD., Southport 4215, Australia. tamaraperchyonok@gmail.com.

ABSTRACT
Healing is a specific biological process related to the general phenomenon of growth and tissue regeneration and is a process generally affected by several systemic conditions or as detrimental side-effects of chemotherapy- and radiotherapy-induced inflammation of the oral mucosa. The objectives of this study is to evaluate the novel chitosan based functional drug delivery systems, which can be successfully incorporated into "dual action bioactive restorative materials", capable of inducing in vitro improved wound healing prototype and containing an antibiotic, such as nystatin, krill oil as an antioxidant and hydroxyapatite as a molecular bone scaffold, which is naturally present in bone and is reported to be successfully used in promoting bone integration when implanted as well as promoting healing. The hydrogels were prepared using a protocol as previously reported by us. The physico-chemical features, including surface morphology (SEM), release behaviors, stability of the therapeutic agent-antioxidant-chitosan, were measured and compared to the earlier reported chitosan-antioxidant containing hydrogels. Structural investigations of the reactive surface of the hydrogel are reported. Release of nystatin was investigated for all newly prepared hydrogels. Bio-adhesive studies were performed in order to assess the suitability of these designer materials. Free radical defense capacity of the biomaterials was evaluated using established in vitro model. The bio-adhesive capacity of the materials in the in vitro system was tested and quantified. It was found that the favorable synergistic effect of free radical built-in defense mechanism of the new functional materials increased sustainable bio-adhesion and therefore acted as a functional multi-dimensional restorative material with potential application in wound healing in vitro.

No MeSH data available.


Related in: MedlinePlus