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The genetics of a putative social trait in natural populations of yeast.

Bozdag GO, Greig D - Mol. Ecol. (2014)

Bottom Line: Here we find that 110 wild yeast strains isolated from natural habitats, and all contained a single SUC locus and produced invertase; none were 'cheats'.The only genetic variants we found were three strains isolated instead from sucrose-rich nectar, which produced higher levels of invertase from three additional SUC loci at their subtelomeres.We argue that the pattern of SUC gene variation is better explained by local adaptation than by social conflict.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Evolutionary Biology, August Thienemann Strasse 2, Plön, 24306, Germany.

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SUC gene copy number detection using droplet digital PCR (ddPCR) in five multilocus strains, normalized to a known single-copy control C. Lab.1 (first column). Three different symbol tones (dark, grey and empty) represent three different biological replicates. Copy number estimates calculated against RPN5 reference probe are on the left-hand side of each column, and copy number estimates calculated against MNN1 are on the right-hand side of each column. Black bars show the means of each set of three biological replicates.
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fig02: SUC gene copy number detection using droplet digital PCR (ddPCR) in five multilocus strains, normalized to a known single-copy control C. Lab.1 (first column). Three different symbol tones (dark, grey and empty) represent three different biological replicates. Copy number estimates calculated against RPN5 reference probe are on the left-hand side of each column, and copy number estimates calculated against MNN1 are on the right-hand side of each column. Black bars show the means of each set of three biological replicates.

Mentions: Our Southern blots showed that all the wild S. paradoxus strains isolated from oak and maple trees contained just a single SUC locus, SUC2, located on chromosome IX. All 27 S. cerevisiae strains isolated from nature also contained SUC2 on chromosome IX, but three S. cerevisiae strains (C.Nectar.1, C.Nectar.2 and C.Nectar.3) contained additional SUC loci on chromosome II (SUC3), on chromosome X (SUC8) and on chromosome XIV (SUC9) (Figs S1 and S2, Supporting information). ddPCR (Fig.2) shows that the SUC copy number of the three wild strains with multiple loci is closest to four, corresponding to one SUC open reading frame for each chromosome with a SUC locus (SUC2, plus the extra loci SUC3, SUC8 and SUC9). All three of these wild strains were isolated from the same environment: Bertam palm (Eugeissona tristis) nectars in West Malaysia (Liti et al. 2009).


The genetics of a putative social trait in natural populations of yeast.

Bozdag GO, Greig D - Mol. Ecol. (2014)

SUC gene copy number detection using droplet digital PCR (ddPCR) in five multilocus strains, normalized to a known single-copy control C. Lab.1 (first column). Three different symbol tones (dark, grey and empty) represent three different biological replicates. Copy number estimates calculated against RPN5 reference probe are on the left-hand side of each column, and copy number estimates calculated against MNN1 are on the right-hand side of each column. Black bars show the means of each set of three biological replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4285311&req=5

fig02: SUC gene copy number detection using droplet digital PCR (ddPCR) in five multilocus strains, normalized to a known single-copy control C. Lab.1 (first column). Three different symbol tones (dark, grey and empty) represent three different biological replicates. Copy number estimates calculated against RPN5 reference probe are on the left-hand side of each column, and copy number estimates calculated against MNN1 are on the right-hand side of each column. Black bars show the means of each set of three biological replicates.
Mentions: Our Southern blots showed that all the wild S. paradoxus strains isolated from oak and maple trees contained just a single SUC locus, SUC2, located on chromosome IX. All 27 S. cerevisiae strains isolated from nature also contained SUC2 on chromosome IX, but three S. cerevisiae strains (C.Nectar.1, C.Nectar.2 and C.Nectar.3) contained additional SUC loci on chromosome II (SUC3), on chromosome X (SUC8) and on chromosome XIV (SUC9) (Figs S1 and S2, Supporting information). ddPCR (Fig.2) shows that the SUC copy number of the three wild strains with multiple loci is closest to four, corresponding to one SUC open reading frame for each chromosome with a SUC locus (SUC2, plus the extra loci SUC3, SUC8 and SUC9). All three of these wild strains were isolated from the same environment: Bertam palm (Eugeissona tristis) nectars in West Malaysia (Liti et al. 2009).

Bottom Line: Here we find that 110 wild yeast strains isolated from natural habitats, and all contained a single SUC locus and produced invertase; none were 'cheats'.The only genetic variants we found were three strains isolated instead from sucrose-rich nectar, which produced higher levels of invertase from three additional SUC loci at their subtelomeres.We argue that the pattern of SUC gene variation is better explained by local adaptation than by social conflict.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Evolutionary Biology, August Thienemann Strasse 2, Plön, 24306, Germany.

Show MeSH