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Detection of Zika virus in urine.

Gourinat AC, O'Connor O, Calvez E, Goarant C, Dupont-Rouzeyrol M - Emerging Infect. Dis. (2015)

Bottom Line: We describe the kinetics of Zika virus (ZIKV) detection in serum and urine samples of 6 patients.Urine samples were positive for ZIKV >10 days after onset of disease, which was a notably longer period than for serum samples.This finding supports the conclusion that urine samples are useful for diagnosis of ZIKV infections.

View Article: PubMed Central - PubMed

ABSTRACT
We describe the kinetics of Zika virus (ZIKV) detection in serum and urine samples of 6 patients. Urine samples were positive for ZIKV >10 days after onset of disease, which was a notably longer period than for serum samples. This finding supports the conclusion that urine samples are useful for diagnosis of ZIKV infections.

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Detection of Zika virus in blood and urine specimens of 6 patients by using real-time reverse transcription PCR with primers/probe 1086/1162c/1107-Cy5 (11) New Caledonia, 2014. A) Patient 1; B) Patient 2; C) Patient 3; D) Patient 4; E) Patient 5; F) Patient 6. Triangles indicate urine samples and squares indicate serum samples. The cutoff cycle threshold (Ct) value is 38.5, as previously reported (11) and is indicated by blue horizontal lines. Black symbols indicate amplifications with Ct <38.5, gray symbols indicate amplifications with Ct ≥38.5, and white symbols indicate negative amplifications. Onset of disease (day 0) was defined retrospectively after questioning patients about initial symptoms. Dashed lines indicate a period >2 days without a sample being obtained. Arrows indicate onset of rash.
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Figure 1: Detection of Zika virus in blood and urine specimens of 6 patients by using real-time reverse transcription PCR with primers/probe 1086/1162c/1107-Cy5 (11) New Caledonia, 2014. A) Patient 1; B) Patient 2; C) Patient 3; D) Patient 4; E) Patient 5; F) Patient 6. Triangles indicate urine samples and squares indicate serum samples. The cutoff cycle threshold (Ct) value is 38.5, as previously reported (11) and is indicated by blue horizontal lines. Black symbols indicate amplifications with Ct <38.5, gray symbols indicate amplifications with Ct ≥38.5, and white symbols indicate negative amplifications. Onset of disease (day 0) was defined retrospectively after questioning patients about initial symptoms. Dashed lines indicate a period >2 days without a sample being obtained. Arrows indicate onset of rash.

Mentions: To detect ZIKV in samples (RNA extracted from 200 μL of serum or urine), we used both sets of primers/probe specific for ZIKV (11). A standard curve with serial dilutions of known concentrations of a ZIKV virus stock was used to estimate viral load in samples. All blood samples were also tested for dengue virus and chikungunya virus by real-time RT-PCR and showed negative results. ZIKV was detected in serum of 4 patients (Figure). Urine samples from 2 other patients were also positive for ZIKV, and showed a higher viral load than corresponding serum samples and were positive for ≤7 days (patient 4) and probably >20 days (patient 3) after viremia reached an undetectable level (Figure). Urine samples from 6 healthy patients were also assessed and showed negative results.


Detection of Zika virus in urine.

Gourinat AC, O'Connor O, Calvez E, Goarant C, Dupont-Rouzeyrol M - Emerging Infect. Dis. (2015)

Detection of Zika virus in blood and urine specimens of 6 patients by using real-time reverse transcription PCR with primers/probe 1086/1162c/1107-Cy5 (11) New Caledonia, 2014. A) Patient 1; B) Patient 2; C) Patient 3; D) Patient 4; E) Patient 5; F) Patient 6. Triangles indicate urine samples and squares indicate serum samples. The cutoff cycle threshold (Ct) value is 38.5, as previously reported (11) and is indicated by blue horizontal lines. Black symbols indicate amplifications with Ct <38.5, gray symbols indicate amplifications with Ct ≥38.5, and white symbols indicate negative amplifications. Onset of disease (day 0) was defined retrospectively after questioning patients about initial symptoms. Dashed lines indicate a period >2 days without a sample being obtained. Arrows indicate onset of rash.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4285245&req=5

Figure 1: Detection of Zika virus in blood and urine specimens of 6 patients by using real-time reverse transcription PCR with primers/probe 1086/1162c/1107-Cy5 (11) New Caledonia, 2014. A) Patient 1; B) Patient 2; C) Patient 3; D) Patient 4; E) Patient 5; F) Patient 6. Triangles indicate urine samples and squares indicate serum samples. The cutoff cycle threshold (Ct) value is 38.5, as previously reported (11) and is indicated by blue horizontal lines. Black symbols indicate amplifications with Ct <38.5, gray symbols indicate amplifications with Ct ≥38.5, and white symbols indicate negative amplifications. Onset of disease (day 0) was defined retrospectively after questioning patients about initial symptoms. Dashed lines indicate a period >2 days without a sample being obtained. Arrows indicate onset of rash.
Mentions: To detect ZIKV in samples (RNA extracted from 200 μL of serum or urine), we used both sets of primers/probe specific for ZIKV (11). A standard curve with serial dilutions of known concentrations of a ZIKV virus stock was used to estimate viral load in samples. All blood samples were also tested for dengue virus and chikungunya virus by real-time RT-PCR and showed negative results. ZIKV was detected in serum of 4 patients (Figure). Urine samples from 2 other patients were also positive for ZIKV, and showed a higher viral load than corresponding serum samples and were positive for ≤7 days (patient 4) and probably >20 days (patient 3) after viremia reached an undetectable level (Figure). Urine samples from 6 healthy patients were also assessed and showed negative results.

Bottom Line: We describe the kinetics of Zika virus (ZIKV) detection in serum and urine samples of 6 patients.Urine samples were positive for ZIKV >10 days after onset of disease, which was a notably longer period than for serum samples.This finding supports the conclusion that urine samples are useful for diagnosis of ZIKV infections.

View Article: PubMed Central - PubMed

ABSTRACT
We describe the kinetics of Zika virus (ZIKV) detection in serum and urine samples of 6 patients. Urine samples were positive for ZIKV >10 days after onset of disease, which was a notably longer period than for serum samples. This finding supports the conclusion that urine samples are useful for diagnosis of ZIKV infections.

Show MeSH
Related in: MedlinePlus