Identification of functionally important conserved trans-membrane residues of bacterial PIB -type ATPases.
Bottom Line: Of the 38 conserved positions tested, 24 had small effects on metal tolerance.Based on structural modelling, the functionally important residues line a constricted 'channel', tightly surrounded by the residues that were found to be inconsequential for function.In addition, by substituting six trans-membrane amino acids of rrZntA we changed the in vivo metal specificity of this pump from Zn(2+)/Cd(2+) to Ag(+).
Affiliation: Department of Microbiology, The Bruce and Ruth Rappaport Faculty of Medicine, The Technion-Israel Institute of Technology, Haifa, Israel.Show MeSH
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Mentions: To verify that the observed effects of the mutations were not specific to growth in liquid media we also performed metal tolerance experiments in solid media. As shown (Fig. 4), largely comparable results were obtained.
Affiliation: Department of Microbiology, The Bruce and Ruth Rappaport Faculty of Medicine, The Technion-Israel Institute of Technology, Haifa, Israel.