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Identification of functionally important conserved trans-membrane residues of bacterial PIB -type ATPases.

Zhitnitsky D, Lewinson O - Mol. Microbiol. (2014)

Bottom Line: Of the 38 conserved positions tested, 24 had small effects on metal tolerance.Based on structural modelling, the functionally important residues line a constricted 'channel', tightly surrounded by the residues that were found to be inconsequential for function.In addition, by substituting six trans-membrane amino acids of rrZntA we changed the in vivo metal specificity of this pump from Zn(2+)/Cd(2+) to Ag(+).

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, The Bruce and Ruth Rappaport Faculty of Medicine, The Technion-Israel Institute of Technology, Haifa, Israel.

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Growth in solid media of wild type and mutant rrZntA. Four 10-fold dilutions were spotted from left to right, in the absence or presence of the indicated concentrations of ZnSO4 or CdCl2.
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fig04: Growth in solid media of wild type and mutant rrZntA. Four 10-fold dilutions were spotted from left to right, in the absence or presence of the indicated concentrations of ZnSO4 or CdCl2.

Mentions: To verify that the observed effects of the mutations were not specific to growth in liquid media we also performed metal tolerance experiments in solid media. As shown (Fig. 4), largely comparable results were obtained.


Identification of functionally important conserved trans-membrane residues of bacterial PIB -type ATPases.

Zhitnitsky D, Lewinson O - Mol. Microbiol. (2014)

Growth in solid media of wild type and mutant rrZntA. Four 10-fold dilutions were spotted from left to right, in the absence or presence of the indicated concentrations of ZnSO4 or CdCl2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4285229&req=5

fig04: Growth in solid media of wild type and mutant rrZntA. Four 10-fold dilutions were spotted from left to right, in the absence or presence of the indicated concentrations of ZnSO4 or CdCl2.
Mentions: To verify that the observed effects of the mutations were not specific to growth in liquid media we also performed metal tolerance experiments in solid media. As shown (Fig. 4), largely comparable results were obtained.

Bottom Line: Of the 38 conserved positions tested, 24 had small effects on metal tolerance.Based on structural modelling, the functionally important residues line a constricted 'channel', tightly surrounded by the residues that were found to be inconsequential for function.In addition, by substituting six trans-membrane amino acids of rrZntA we changed the in vivo metal specificity of this pump from Zn(2+)/Cd(2+) to Ag(+).

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, The Bruce and Ruth Rappaport Faculty of Medicine, The Technion-Israel Institute of Technology, Haifa, Israel.

Show MeSH
Related in: MedlinePlus