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Expression of glucagon-like Peptide-1 receptor in papillary thyroid carcinoma and its clinicopathologic significance.

Jung MJ, Kwon SK - Endocrinol Metab (Seoul) (2014)

Bottom Line: Considering the large numbers of papillary thyroid carcinomas (PTCs) and possible effects of glucagon-like peptide-1 (GLP-1) on cell proliferation, the expression of GLP-1 receptor (GLP-1R) in PTC is likely to have clinical significance.Extrathyroidal extension showed positive association with GLP-1R expression that was almost significant.Sex, age, tumor size, and lymph node metastasis were not significantly associated with GLP-1R expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Kosin University Gospel Hospital, Kosin University College of Medicine, Busan, Korea.

ABSTRACT

Background: Incretin-based therapies are rapidly becoming one of the main glycemic control strategies in diabetes. Considering the large numbers of papillary thyroid carcinomas (PTCs) and possible effects of glucagon-like peptide-1 (GLP-1) on cell proliferation, the expression of GLP-1 receptor (GLP-1R) in PTC is likely to have clinical significance. We performed this study to evaluate the expression of GLP-1R in PTC and the clinical meaning of GLP-1R expression in PTC.

Methods: Fifty-six cases of PTC, four cases of medullary thyroid cancer (MTC), seven cases of nodular hyperplasia and 56 normal thyroid tissue samples were selected for immunostaining for GLP-1R. Clinical parameters were obtained by retrospective review of medical records.

Results: Immunohistochemical staining for GLP-1R showed immunoreactivity in 18 of 56 cases of PTC (32.1%). All four cases of MTC exhibited cytoplasmic GLP-1R expression. Nodular hyperplasia exhibited immunoreactivity in two of seven cases (28.6%). All normal thyroid follicular cells showed negative immunoreactivity. In univariable and multivariable analyses, tumor multifocality was negatively correlated with GLP-1R expression. Extrathyroidal extension showed positive association with GLP-1R expression that was almost significant. Sex, age, tumor size, and lymph node metastasis were not significantly associated with GLP-1R expression.

Conclusion: Some parts of PTC tissues express GLP-1R, and GLP-1R expression in PTC was negatively correlated with tumor multifocality. The long-term influence of pharmacologically increased GLP-1 on thyroid follicular cells and development and progression of tumors originating from thyroid follicular cells should be investigated.

No MeSH data available.


Related in: MedlinePlus

Glucagon-like peptide-1 receptor (GLP-1R) expression in medullary thyroid carcinoma and nodular hyperplasia. (A) and (B) show medullary thyroid carcinoma samples positive for GLP-1R, with strong (A) and mixed strong and weak (×400) (B) immunoreactivity (×400). (C) and (D) show nodular hyperplasia samples positive for GLP-1R. (C) Strong immunoreactivity (×400). (D) Weak immunoreactivity (×400).
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Figure 2: Glucagon-like peptide-1 receptor (GLP-1R) expression in medullary thyroid carcinoma and nodular hyperplasia. (A) and (B) show medullary thyroid carcinoma samples positive for GLP-1R, with strong (A) and mixed strong and weak (×400) (B) immunoreactivity (×400). (C) and (D) show nodular hyperplasia samples positive for GLP-1R. (C) Strong immunoreactivity (×400). (D) Weak immunoreactivity (×400).

Mentions: Immunohistochemical staining of GLP-1R in PTC showed cytoplasmic expression in 18 of 56 cases (32.1%) (Table 1). The expression of GLP-1R was strong in two cases (100% and 80% intensity) and weak in 16 cases, in which the distribution was usually diffuse and over 50%, except for two cases with distributions of 20%. All four cases of MTC exhibited strong cytoplasmic expression of GLP-1R, albeit the staining was focal (Table 1, Fig. 2A, B). The mean serum calcitonin level was 1.65±1.78 in the PTC group and 629.3±314.7 pg/mL in the MTC group. There was strong cytoplasmic expression of GLP-1R in two of seven cases of nodular hyperplasia (28.6%) (Table 1, Fig. 2C, D). Very few non-neoplastic follicular cells in the adjacent thyroid parenchyma were positive for GLP-1R (less than 5% of the distribution) or exhibited ambiguous granules in contact with colloid showing non-specific immunoreactivity (Fig. 3). These findings were insufficient to interpret as positive immunoreactivity. Some cases showed strong GLP-1R expression in C cells of the adjacent thyroid parenchyma (Fig. 3A).


Expression of glucagon-like Peptide-1 receptor in papillary thyroid carcinoma and its clinicopathologic significance.

Jung MJ, Kwon SK - Endocrinol Metab (Seoul) (2014)

Glucagon-like peptide-1 receptor (GLP-1R) expression in medullary thyroid carcinoma and nodular hyperplasia. (A) and (B) show medullary thyroid carcinoma samples positive for GLP-1R, with strong (A) and mixed strong and weak (×400) (B) immunoreactivity (×400). (C) and (D) show nodular hyperplasia samples positive for GLP-1R. (C) Strong immunoreactivity (×400). (D) Weak immunoreactivity (×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4285044&req=5

Figure 2: Glucagon-like peptide-1 receptor (GLP-1R) expression in medullary thyroid carcinoma and nodular hyperplasia. (A) and (B) show medullary thyroid carcinoma samples positive for GLP-1R, with strong (A) and mixed strong and weak (×400) (B) immunoreactivity (×400). (C) and (D) show nodular hyperplasia samples positive for GLP-1R. (C) Strong immunoreactivity (×400). (D) Weak immunoreactivity (×400).
Mentions: Immunohistochemical staining of GLP-1R in PTC showed cytoplasmic expression in 18 of 56 cases (32.1%) (Table 1). The expression of GLP-1R was strong in two cases (100% and 80% intensity) and weak in 16 cases, in which the distribution was usually diffuse and over 50%, except for two cases with distributions of 20%. All four cases of MTC exhibited strong cytoplasmic expression of GLP-1R, albeit the staining was focal (Table 1, Fig. 2A, B). The mean serum calcitonin level was 1.65±1.78 in the PTC group and 629.3±314.7 pg/mL in the MTC group. There was strong cytoplasmic expression of GLP-1R in two of seven cases of nodular hyperplasia (28.6%) (Table 1, Fig. 2C, D). Very few non-neoplastic follicular cells in the adjacent thyroid parenchyma were positive for GLP-1R (less than 5% of the distribution) or exhibited ambiguous granules in contact with colloid showing non-specific immunoreactivity (Fig. 3). These findings were insufficient to interpret as positive immunoreactivity. Some cases showed strong GLP-1R expression in C cells of the adjacent thyroid parenchyma (Fig. 3A).

Bottom Line: Considering the large numbers of papillary thyroid carcinomas (PTCs) and possible effects of glucagon-like peptide-1 (GLP-1) on cell proliferation, the expression of GLP-1 receptor (GLP-1R) in PTC is likely to have clinical significance.Extrathyroidal extension showed positive association with GLP-1R expression that was almost significant.Sex, age, tumor size, and lymph node metastasis were not significantly associated with GLP-1R expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Kosin University Gospel Hospital, Kosin University College of Medicine, Busan, Korea.

ABSTRACT

Background: Incretin-based therapies are rapidly becoming one of the main glycemic control strategies in diabetes. Considering the large numbers of papillary thyroid carcinomas (PTCs) and possible effects of glucagon-like peptide-1 (GLP-1) on cell proliferation, the expression of GLP-1 receptor (GLP-1R) in PTC is likely to have clinical significance. We performed this study to evaluate the expression of GLP-1R in PTC and the clinical meaning of GLP-1R expression in PTC.

Methods: Fifty-six cases of PTC, four cases of medullary thyroid cancer (MTC), seven cases of nodular hyperplasia and 56 normal thyroid tissue samples were selected for immunostaining for GLP-1R. Clinical parameters were obtained by retrospective review of medical records.

Results: Immunohistochemical staining for GLP-1R showed immunoreactivity in 18 of 56 cases of PTC (32.1%). All four cases of MTC exhibited cytoplasmic GLP-1R expression. Nodular hyperplasia exhibited immunoreactivity in two of seven cases (28.6%). All normal thyroid follicular cells showed negative immunoreactivity. In univariable and multivariable analyses, tumor multifocality was negatively correlated with GLP-1R expression. Extrathyroidal extension showed positive association with GLP-1R expression that was almost significant. Sex, age, tumor size, and lymph node metastasis were not significantly associated with GLP-1R expression.

Conclusion: Some parts of PTC tissues express GLP-1R, and GLP-1R expression in PTC was negatively correlated with tumor multifocality. The long-term influence of pharmacologically increased GLP-1 on thyroid follicular cells and development and progression of tumors originating from thyroid follicular cells should be investigated.

No MeSH data available.


Related in: MedlinePlus