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Transcriptome sequencing in a Tibetan barley landrace with high resistance to powdery mildew.

Zeng XQ, Luo XM, Wang YL, Xu QJ, Bai LJ, Yuan HJ, Tashi N - ScientificWorldJournal (2014)

Bottom Line: A total of 831 significant differentially expressed genes were found in the infected seedlings, covering 19 functions.In addition, 330 KEGG pathways were found using BLASTx with an E-value cut-off of <10(-5).Among them, three pathways, namely, "photosynthesis," "plant-pathogen interaction," and "photosynthesis-antenna proteins" had significant matches in the database.

View Article: PubMed Central - PubMed

Affiliation: Barley Improvement and Yak Breeding Key Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China.

ABSTRACT
Hulless barley is an important cereal crop worldwide, especially in Tibet of China. However, this crop is usually susceptible to powdery mildew caused by Blumeria graminis f. sp. hordei. In this study, we aimed to understand the functions and pathways of genes involved in the disease resistance by transcriptome sequencing of a Tibetan barley landrace with high resistance to powdery mildew. A total of 831 significant differentially expressed genes were found in the infected seedlings, covering 19 functions. Either "cell," "cell part," and "extracellular region" in the cellular component category or "binding" and "catalytic" in the category of molecular function as well as "metabolic process" and "cellular process" in the biological process category together demonstrated that these functions may be involved in the resistance to powdery mildew of the hulless barley. In addition, 330 KEGG pathways were found using BLASTx with an E-value cut-off of <10(-5). Among them, three pathways, namely, "photosynthesis," "plant-pathogen interaction," and "photosynthesis-antenna proteins" had significant matches in the database. Significant expressions of the three pathways were detected at 24 h, 48 h, and 96 h after infection, respectively. These results indicated a complex process of barley response to powdery mildew infection.

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Related in: MedlinePlus

Mapping reads (exon, intron, and intergene) coverage of A (C0, TR130348).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4284937&req=5

fig1: Mapping reads (exon, intron, and intergene) coverage of A (C0, TR130348).

Mentions: Based on the mapped reads, the proportion of exon mapping, intron mapping, and intergene mapping of sample A at 0 h since infection 5 were illustrated in Figure 1. The highest exon mapping (60.3%) was found in Sample A, while the lowest (52.1%) was found in Sample F (C120, TR130353). The intron mapping coverage ranged from 8.3% (A) to 10.8% (B, C24, TR130349). The average of intergene mapping was 35.2%. There was no affinity with reference genome annotation.


Transcriptome sequencing in a Tibetan barley landrace with high resistance to powdery mildew.

Zeng XQ, Luo XM, Wang YL, Xu QJ, Bai LJ, Yuan HJ, Tashi N - ScientificWorldJournal (2014)

Mapping reads (exon, intron, and intergene) coverage of A (C0, TR130348).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4284937&req=5

fig1: Mapping reads (exon, intron, and intergene) coverage of A (C0, TR130348).
Mentions: Based on the mapped reads, the proportion of exon mapping, intron mapping, and intergene mapping of sample A at 0 h since infection 5 were illustrated in Figure 1. The highest exon mapping (60.3%) was found in Sample A, while the lowest (52.1%) was found in Sample F (C120, TR130353). The intron mapping coverage ranged from 8.3% (A) to 10.8% (B, C24, TR130349). The average of intergene mapping was 35.2%. There was no affinity with reference genome annotation.

Bottom Line: A total of 831 significant differentially expressed genes were found in the infected seedlings, covering 19 functions.In addition, 330 KEGG pathways were found using BLASTx with an E-value cut-off of <10(-5).Among them, three pathways, namely, "photosynthesis," "plant-pathogen interaction," and "photosynthesis-antenna proteins" had significant matches in the database.

View Article: PubMed Central - PubMed

Affiliation: Barley Improvement and Yak Breeding Key Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China.

ABSTRACT
Hulless barley is an important cereal crop worldwide, especially in Tibet of China. However, this crop is usually susceptible to powdery mildew caused by Blumeria graminis f. sp. hordei. In this study, we aimed to understand the functions and pathways of genes involved in the disease resistance by transcriptome sequencing of a Tibetan barley landrace with high resistance to powdery mildew. A total of 831 significant differentially expressed genes were found in the infected seedlings, covering 19 functions. Either "cell," "cell part," and "extracellular region" in the cellular component category or "binding" and "catalytic" in the category of molecular function as well as "metabolic process" and "cellular process" in the biological process category together demonstrated that these functions may be involved in the resistance to powdery mildew of the hulless barley. In addition, 330 KEGG pathways were found using BLASTx with an E-value cut-off of <10(-5). Among them, three pathways, namely, "photosynthesis," "plant-pathogen interaction," and "photosynthesis-antenna proteins" had significant matches in the database. Significant expressions of the three pathways were detected at 24 h, 48 h, and 96 h after infection, respectively. These results indicated a complex process of barley response to powdery mildew infection.

Show MeSH
Related in: MedlinePlus