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Determination of oxidative stress related toxicity on repeated dermal exposure of hydroxyapatite nanoparticles in rats.

Parayanthala Valappil M, Santhakumar S, Arumugam S - Int J Biomater (2014)

Bottom Line: Gross examination of carcasses did not reveal any abnormality in the organs examined.The results also demonstrated that there was no significant fluctuation in the level of antioxidant defense mechanisms, lipid peroxidation, and haematological and biochemical parameters.Hence, it can be concluded that the synthesized HANPs were nontoxic at cellular level, when exposed dermally to rats.

View Article: PubMed Central - PubMed

Affiliation: Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695 012, India.

ABSTRACT
Hydroxyapatite nanoparticles (HANPs) have numerous applications, such as substitute for bone grafting, bone fillers, bioceramic coating, and dental fillings. The toxicity of these nanomaterials is of growing concern despite their significant scientific interest and promising potential in many applications. In this study, an in-house synthesized, characterized HANP of size <50 nm was investigated for the dermal toxicity. A paste of HANPs was prepared in water and applied on the dorsal side of the rats for 28 days. At the end of 28 days, blood was subjected to haematological and biochemical analysis. Gross necropsy was conducted and major organs were collected for histopathological observations. Liver from the animals was evaluated for lipid peroxidation, reduced glutathione, and antioxidant enzymes activity. It was observed that none of the animals showed any abnormality during the experimental period. Gross examination of carcasses did not reveal any abnormality in the organs examined. The results also demonstrated that there was no significant fluctuation in the level of antioxidant defense mechanisms, lipid peroxidation, and haematological and biochemical parameters. There was no histopathological lesion observed in any of the organs. Hence, it can be concluded that the synthesized HANPs were nontoxic at cellular level, when exposed dermally to rats.

No MeSH data available.


Related in: MedlinePlus

TEM image of an in-house synthesized HANP.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4283387&req=5

fig1: TEM image of an in-house synthesized HANP.

Mentions: HANPs were synthesized by wet chemical method. These particles were characterized by transmission electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectral analysis, zeta potential measurements, and SEM/EDS ( ). The transmission electron microscopic data indicated in Figure 1 suggested that the size of synthesized HANPs was found to be below 50 nm.


Determination of oxidative stress related toxicity on repeated dermal exposure of hydroxyapatite nanoparticles in rats.

Parayanthala Valappil M, Santhakumar S, Arumugam S - Int J Biomater (2014)

TEM image of an in-house synthesized HANP.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4283387&req=5

fig1: TEM image of an in-house synthesized HANP.
Mentions: HANPs were synthesized by wet chemical method. These particles were characterized by transmission electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectral analysis, zeta potential measurements, and SEM/EDS ( ). The transmission electron microscopic data indicated in Figure 1 suggested that the size of synthesized HANPs was found to be below 50 nm.

Bottom Line: Gross examination of carcasses did not reveal any abnormality in the organs examined.The results also demonstrated that there was no significant fluctuation in the level of antioxidant defense mechanisms, lipid peroxidation, and haematological and biochemical parameters.Hence, it can be concluded that the synthesized HANPs were nontoxic at cellular level, when exposed dermally to rats.

View Article: PubMed Central - PubMed

Affiliation: Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695 012, India.

ABSTRACT
Hydroxyapatite nanoparticles (HANPs) have numerous applications, such as substitute for bone grafting, bone fillers, bioceramic coating, and dental fillings. The toxicity of these nanomaterials is of growing concern despite their significant scientific interest and promising potential in many applications. In this study, an in-house synthesized, characterized HANP of size <50 nm was investigated for the dermal toxicity. A paste of HANPs was prepared in water and applied on the dorsal side of the rats for 28 days. At the end of 28 days, blood was subjected to haematological and biochemical analysis. Gross necropsy was conducted and major organs were collected for histopathological observations. Liver from the animals was evaluated for lipid peroxidation, reduced glutathione, and antioxidant enzymes activity. It was observed that none of the animals showed any abnormality during the experimental period. Gross examination of carcasses did not reveal any abnormality in the organs examined. The results also demonstrated that there was no significant fluctuation in the level of antioxidant defense mechanisms, lipid peroxidation, and haematological and biochemical parameters. There was no histopathological lesion observed in any of the organs. Hence, it can be concluded that the synthesized HANPs were nontoxic at cellular level, when exposed dermally to rats.

No MeSH data available.


Related in: MedlinePlus