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Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction.

Guo Y, Fukuda T, Nakamura S, Bai L, Xu J, Kuroda K, Tomioka R, Yoneyama H, Isogai E - Asian-australas. J. Anim. Sci. (2015)

Bottom Line: Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines.In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells.Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

View Article: PubMed Central - PubMed

Affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan ; Department of Immunobiology and Pathogenic Biology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.

ABSTRACT
Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

No MeSH data available.


Related in: MedlinePlus

Detection of TLR4 mRNA expression on different kinds of pig cells by qRT-PCR analysis. Lane 1: pig peripheral blood mononuclear cells (PBMCs), lane 2: pig embryonic fibroblast cell line (PEFs_NCC1), lane 3: pig embryonic fibroblast cell line (PEFs_SV40), M: marker (100 bp DNA ladder). TLR4, toll-like receptor 4; qRT-PCR, quantitative real-time polymerase chain reaction.
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f1-ajas-28-2-273: Detection of TLR4 mRNA expression on different kinds of pig cells by qRT-PCR analysis. Lane 1: pig peripheral blood mononuclear cells (PBMCs), lane 2: pig embryonic fibroblast cell line (PEFs_NCC1), lane 3: pig embryonic fibroblast cell line (PEFs_SV40), M: marker (100 bp DNA ladder). TLR4, toll-like receptor 4; qRT-PCR, quantitative real-time polymerase chain reaction.

Mentions: The TLR2 and TLR4 mRNA expressions were analyzed in cultured PEFs_SV40 cells. The TLR4 mRNA with the size of 125 bp expressed in PBMCs and pig fibroblasts (PEFs_NCC1), but not in PEFs_SV40 cells (Figure 1). Thus, the PEFs_SV40 cells which do not express TLR4 mRNA have a potential to be used as a good model in researches on immune response in which TLR2 is involved.


Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction.

Guo Y, Fukuda T, Nakamura S, Bai L, Xu J, Kuroda K, Tomioka R, Yoneyama H, Isogai E - Asian-australas. J. Anim. Sci. (2015)

Detection of TLR4 mRNA expression on different kinds of pig cells by qRT-PCR analysis. Lane 1: pig peripheral blood mononuclear cells (PBMCs), lane 2: pig embryonic fibroblast cell line (PEFs_NCC1), lane 3: pig embryonic fibroblast cell line (PEFs_SV40), M: marker (100 bp DNA ladder). TLR4, toll-like receptor 4; qRT-PCR, quantitative real-time polymerase chain reaction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4283174&req=5

f1-ajas-28-2-273: Detection of TLR4 mRNA expression on different kinds of pig cells by qRT-PCR analysis. Lane 1: pig peripheral blood mononuclear cells (PBMCs), lane 2: pig embryonic fibroblast cell line (PEFs_NCC1), lane 3: pig embryonic fibroblast cell line (PEFs_SV40), M: marker (100 bp DNA ladder). TLR4, toll-like receptor 4; qRT-PCR, quantitative real-time polymerase chain reaction.
Mentions: The TLR2 and TLR4 mRNA expressions were analyzed in cultured PEFs_SV40 cells. The TLR4 mRNA with the size of 125 bp expressed in PBMCs and pig fibroblasts (PEFs_NCC1), but not in PEFs_SV40 cells (Figure 1). Thus, the PEFs_SV40 cells which do not express TLR4 mRNA have a potential to be used as a good model in researches on immune response in which TLR2 is involved.

Bottom Line: Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines.In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells.Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

View Article: PubMed Central - PubMed

Affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan ; Department of Immunobiology and Pathogenic Biology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.

ABSTRACT
Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

No MeSH data available.


Related in: MedlinePlus