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Differential Expression Patterns of Crystallin Genes during Ocular Development of Olive Flounder (Paralichthys olivaceus).

Yang H, Lee YM, Noh JK, Kim HC, Park CJ, Park JW, Hwang IJ, Kim SY, Lee JH - (2012)

Bottom Line: Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea.For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder.The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.

View Article: PubMed Central - PubMed

Affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.

ABSTRACT
Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea. Although olive flounder receive attention from aquaculture and fisheries and extensive research has been conducted eye morphological change in metamorphosis, but little information was known to molecular mechanism and gene expression of eye development- related genes during the early part of eye formation period. For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder. Through the search of an olive flounder DNA database of expressed sequence tags (EST), we found a partial sequence that was similar to crystallin beta A1 and gamma S. Microscopic observation of retinal formation correspond with the time of expression of the crystallin beta A1 and gamma S gene in the developmental stage, these result suggesting that beta A1 and gamma S play a vital role in the remodeling of the retina during eye development. The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.

No MeSH data available.


Related in: MedlinePlus

Expression of crystallin genes induces from several tissues in the olive flounder. Experiments are separated by periods of adult fish. Crystallin genes expression from the segmental tissue (B: brain, M: muscle, F: fin, E: eye, L: liver, S: spleen, K: kidney, G: gill, N: negative) for the olive flounder at 90 days, 10 month and 2 years of age were analyzed using RT-PCR. All results are representative of three independent experiments.
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Figure 4: Expression of crystallin genes induces from several tissues in the olive flounder. Experiments are separated by periods of adult fish. Crystallin genes expression from the segmental tissue (B: brain, M: muscle, F: fin, E: eye, L: liver, S: spleen, K: kidney, G: gill, N: negative) for the olive flounder at 90 days, 10 month and 2 years of age were analyzed using RT-PCR. All results are representative of three independent experiments.

Mentions: For analyzing the stage and tissue-specific expression of crystallin beta A1 and crystallin gamma S in olive flounder, we were sampled various tissue from different development stage (90 days, 10 month and 2 years olive flounder). As shown in Fig. 4, the expression of crystallin beta A1 and gamma S were obviously strong in the eye than other tissue at all tested developing stage, although were ubiquitiously expressed with weak level in all tested organ except kidney.


Differential Expression Patterns of Crystallin Genes during Ocular Development of Olive Flounder (Paralichthys olivaceus).

Yang H, Lee YM, Noh JK, Kim HC, Park CJ, Park JW, Hwang IJ, Kim SY, Lee JH - (2012)

Expression of crystallin genes induces from several tissues in the olive flounder. Experiments are separated by periods of adult fish. Crystallin genes expression from the segmental tissue (B: brain, M: muscle, F: fin, E: eye, L: liver, S: spleen, K: kidney, G: gill, N: negative) for the olive flounder at 90 days, 10 month and 2 years of age were analyzed using RT-PCR. All results are representative of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4282235&req=5

Figure 4: Expression of crystallin genes induces from several tissues in the olive flounder. Experiments are separated by periods of adult fish. Crystallin genes expression from the segmental tissue (B: brain, M: muscle, F: fin, E: eye, L: liver, S: spleen, K: kidney, G: gill, N: negative) for the olive flounder at 90 days, 10 month and 2 years of age were analyzed using RT-PCR. All results are representative of three independent experiments.
Mentions: For analyzing the stage and tissue-specific expression of crystallin beta A1 and crystallin gamma S in olive flounder, we were sampled various tissue from different development stage (90 days, 10 month and 2 years olive flounder). As shown in Fig. 4, the expression of crystallin beta A1 and gamma S were obviously strong in the eye than other tissue at all tested developing stage, although were ubiquitiously expressed with weak level in all tested organ except kidney.

Bottom Line: Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea.For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder.The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.

View Article: PubMed Central - PubMed

Affiliation: Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.

ABSTRACT
Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea. Although olive flounder receive attention from aquaculture and fisheries and extensive research has been conducted eye morphological change in metamorphosis, but little information was known to molecular mechanism and gene expression of eye development- related genes during the early part of eye formation period. For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder. Through the search of an olive flounder DNA database of expressed sequence tags (EST), we found a partial sequence that was similar to crystallin beta A1 and gamma S. Microscopic observation of retinal formation correspond with the time of expression of the crystallin beta A1 and gamma S gene in the developmental stage, these result suggesting that beta A1 and gamma S play a vital role in the remodeling of the retina during eye development. The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.

No MeSH data available.


Related in: MedlinePlus