Limits...
Effect of manganese exposure on the reproductive organs in immature female rats.

Kim SI, Jang YS, Han SH, Choi MJ, Go EH, Cheon YP, Lee JS, Lee SH - (2012)

Bottom Line: Uterine tissue weights of 3.3 mg dose MnCl2 groups were significantly lower than those of control animals (p<0.01), while the 1 mg MnCl2 dose and 10 mg MnCl2 dose failed to induce any change in uterine weight.Similarly, only 3.3 mg MnCl2 dose could induce the significant decrease in the oviduct weight compared to the control group (p<0.05).Combined with the well-defined actions of this metal on GnRH and prolactin secretion, one can suggest the Mn(2+) might be a potential environmental mediator which is involved in the female pubertal process.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Green Life Science, Sangmyung University, Seoul 110-743, Korea.

ABSTRACT
Manganese (Mn(2+)) is a trace element that is essential for normal physiology, and is predominantly obtained from food. Several lines of evidence, however, demonstrated that overexposure to MnCl2 exerts serious neurotoxicity, immunotoxicity and developmental toxicity, particularly in male. The present study aimed to evaluate the effect of 0, 1.0, 3.3, and 10 mg/kg/day doses of MnCl2 on the reproductive organs in the immature female rats. Rats (PND 22; S.D. strain) were exposed to MnCl2 (MnCl2 ∙ 4H2O) dissolved in drinking water for 2 weeks. The animals were sacrificed on PND 35, then the tissues were immediately removed and weighed. Histological studies were performed using the uteri tissue samples. Serum LH and FSH levels were measured with the specific ELISA kits. Body weights of the experimental group animals were not significantly different from those of control group animals. However, ovarian tissue weights in 1 mg and 3.3 mg MnCl2 dose groups were significantly lower than those of control animals (p<0.05 and p<0.01, respectively). Uterine tissue weights of 3.3 mg dose MnCl2 groups were significantly lower than those of control animals (p<0.01), while the 1 mg MnCl2 dose and 10 mg MnCl2 dose failed to induce any change in uterine weight. Similarly, only 3.3 mg MnCl2 dose could induce the significant decrease in the oviduct weight compared to the control group (p<0.05). Non-reproductive tissues such as adrenal and kidney failed to respond to all doses of MnCl2 exposure. The uterine histology revealed that the MnCl2 exposure could affect the myometrial cell proliferation particularly in 3.3 mg dose and 10mg dose group. Serum FSH levels were significantly decreased in 1mg MnCl2 dose and 10 MnCl2 mg groups (p<0.05 and p<0.01, respectively). In contrast, treatment with 1 mg MnCl2 dose induced a significant increment of serum LH level (p<0.05). The present study demonstrated that MnCl2 exposure is capable of inducing abnormal development of reproductive tissues, at least to some extent, and altered gonadotropin secretions in immature female rats. Combined with the well-defined actions of this metal on GnRH and prolactin secretion, one can suggest the Mn(2+) might be a potential environmental mediator which is involved in the female pubertal process.

No MeSH data available.


Related in: MedlinePlus

Measurement of serum FSH and LH levels in response to Mn2+ exposure. The trunk blood samples were collected and centrifuged at 3,000×g for 15 min. The measurements of the serum FSH and LH were carried out according to the commercial instructions for the specific ELISA kits (USCN, China). Values are expressed as mean±S.E. (n=6 per group). *, Significantly different from control group, p<0.05. **, Significantly different from control group, p<0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4282234&req=5

Figure 3: Measurement of serum FSH and LH levels in response to Mn2+ exposure. The trunk blood samples were collected and centrifuged at 3,000×g for 15 min. The measurements of the serum FSH and LH were carried out according to the commercial instructions for the specific ELISA kits (USCN, China). Values are expressed as mean±S.E. (n=6 per group). *, Significantly different from control group, p<0.05. **, Significantly different from control group, p<0.01.

Mentions: Serum LH and FSH levels were measured using specific ELISA kits. Fig. 3 (A) shows that the secretion of FSH was significantly decreased by treatment with 1 mg or 3.3 mg MnCl2 (Control:1 mg dose MnCl2 = 3.54±0.18 : 3.07±0.14 ng/ml, p<0.05; Control : 10 mg dose MnCl2 = 3.54±0.18:2.75±0.10 ng/ml, p<0.01). Serum LH level was significantly elevated by 1 mg MnCl2 exposure (Control : 1 mg dose MnCl2 = 39.85±8.93:76.71±12.36 ng/ml, p<0.05, Fig. 3, B). Higher doses of MnCl2 exposure failed to change the serum LH levels.


Effect of manganese exposure on the reproductive organs in immature female rats.

Kim SI, Jang YS, Han SH, Choi MJ, Go EH, Cheon YP, Lee JS, Lee SH - (2012)

Measurement of serum FSH and LH levels in response to Mn2+ exposure. The trunk blood samples were collected and centrifuged at 3,000×g for 15 min. The measurements of the serum FSH and LH were carried out according to the commercial instructions for the specific ELISA kits (USCN, China). Values are expressed as mean±S.E. (n=6 per group). *, Significantly different from control group, p<0.05. **, Significantly different from control group, p<0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4282234&req=5

Figure 3: Measurement of serum FSH and LH levels in response to Mn2+ exposure. The trunk blood samples were collected and centrifuged at 3,000×g for 15 min. The measurements of the serum FSH and LH were carried out according to the commercial instructions for the specific ELISA kits (USCN, China). Values are expressed as mean±S.E. (n=6 per group). *, Significantly different from control group, p<0.05. **, Significantly different from control group, p<0.01.
Mentions: Serum LH and FSH levels were measured using specific ELISA kits. Fig. 3 (A) shows that the secretion of FSH was significantly decreased by treatment with 1 mg or 3.3 mg MnCl2 (Control:1 mg dose MnCl2 = 3.54±0.18 : 3.07±0.14 ng/ml, p<0.05; Control : 10 mg dose MnCl2 = 3.54±0.18:2.75±0.10 ng/ml, p<0.01). Serum LH level was significantly elevated by 1 mg MnCl2 exposure (Control : 1 mg dose MnCl2 = 39.85±8.93:76.71±12.36 ng/ml, p<0.05, Fig. 3, B). Higher doses of MnCl2 exposure failed to change the serum LH levels.

Bottom Line: Uterine tissue weights of 3.3 mg dose MnCl2 groups were significantly lower than those of control animals (p<0.01), while the 1 mg MnCl2 dose and 10 mg MnCl2 dose failed to induce any change in uterine weight.Similarly, only 3.3 mg MnCl2 dose could induce the significant decrease in the oviduct weight compared to the control group (p<0.05).Combined with the well-defined actions of this metal on GnRH and prolactin secretion, one can suggest the Mn(2+) might be a potential environmental mediator which is involved in the female pubertal process.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Green Life Science, Sangmyung University, Seoul 110-743, Korea.

ABSTRACT
Manganese (Mn(2+)) is a trace element that is essential for normal physiology, and is predominantly obtained from food. Several lines of evidence, however, demonstrated that overexposure to MnCl2 exerts serious neurotoxicity, immunotoxicity and developmental toxicity, particularly in male. The present study aimed to evaluate the effect of 0, 1.0, 3.3, and 10 mg/kg/day doses of MnCl2 on the reproductive organs in the immature female rats. Rats (PND 22; S.D. strain) were exposed to MnCl2 (MnCl2 ∙ 4H2O) dissolved in drinking water for 2 weeks. The animals were sacrificed on PND 35, then the tissues were immediately removed and weighed. Histological studies were performed using the uteri tissue samples. Serum LH and FSH levels were measured with the specific ELISA kits. Body weights of the experimental group animals were not significantly different from those of control group animals. However, ovarian tissue weights in 1 mg and 3.3 mg MnCl2 dose groups were significantly lower than those of control animals (p<0.05 and p<0.01, respectively). Uterine tissue weights of 3.3 mg dose MnCl2 groups were significantly lower than those of control animals (p<0.01), while the 1 mg MnCl2 dose and 10 mg MnCl2 dose failed to induce any change in uterine weight. Similarly, only 3.3 mg MnCl2 dose could induce the significant decrease in the oviduct weight compared to the control group (p<0.05). Non-reproductive tissues such as adrenal and kidney failed to respond to all doses of MnCl2 exposure. The uterine histology revealed that the MnCl2 exposure could affect the myometrial cell proliferation particularly in 3.3 mg dose and 10mg dose group. Serum FSH levels were significantly decreased in 1mg MnCl2 dose and 10 MnCl2 mg groups (p<0.05 and p<0.01, respectively). In contrast, treatment with 1 mg MnCl2 dose induced a significant increment of serum LH level (p<0.05). The present study demonstrated that MnCl2 exposure is capable of inducing abnormal development of reproductive tissues, at least to some extent, and altered gonadotropin secretions in immature female rats. Combined with the well-defined actions of this metal on GnRH and prolactin secretion, one can suggest the Mn(2+) might be a potential environmental mediator which is involved in the female pubertal process.

No MeSH data available.


Related in: MedlinePlus