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Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging.

Park MJ, Park SH, Moon SE, Koo JS, Moon HS, Joo BS - (2013)

Bottom Line: After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry.Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4.This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.

View Article: PubMed Central - PubMed

Affiliation: Center for Reproductive Medicine, Good Moonhwa Hospital, Busan 601-803, Korea.

ABSTRACT
This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6-9, 14-16, and 23-26 weeks) were injected with 5 IU pregnant mare's serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23-26 weeks compared with the young mice of 6-9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.

No MeSH data available.


Representative densitometry analysis of western blot for the expressions of Id-1 and VEGF in ovaries of different aged mice. Actin expression was used as control. Densitometry analysis of expressed Id-1 and VEGF content was performed using Image J program and normalized by the control level. Data are the mean±SEM. *P < 0.05, **P<0.005 (vs. 6–9 weeks group).
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Figure 2: Representative densitometry analysis of western blot for the expressions of Id-1 and VEGF in ovaries of different aged mice. Actin expression was used as control. Densitometry analysis of expressed Id-1 and VEGF content was performed using Image J program and normalized by the control level. Data are the mean±SEM. *P < 0.05, **P<0.005 (vs. 6–9 weeks group).

Mentions: Next, to examine whether these mRNA expression profiles show the same pattern in the protein level, we carried out western blot analysis for protein expressions of only Id-1 and VEGF because the mRNA expressions of these two factors resulted in a significantly decrease. As shown in RT-PCR experiment, both protein levels of Id-1 and VEGF in the ovary were also significantly decreased with advancing female age (P<0.05). However, unlike mRNA expression results, the expression of Id-1 and VEGF at the protein level began to significantly decrease from the middle age group of 14 ‒ 16 weeks old, and the levels of decrease were more obvious in 23 ‒ 26 weeks old (Fig. 2).


Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging.

Park MJ, Park SH, Moon SE, Koo JS, Moon HS, Joo BS - (2013)

Representative densitometry analysis of western blot for the expressions of Id-1 and VEGF in ovaries of different aged mice. Actin expression was used as control. Densitometry analysis of expressed Id-1 and VEGF content was performed using Image J program and normalized by the control level. Data are the mean±SEM. *P < 0.05, **P<0.005 (vs. 6–9 weeks group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4282215&req=5

Figure 2: Representative densitometry analysis of western blot for the expressions of Id-1 and VEGF in ovaries of different aged mice. Actin expression was used as control. Densitometry analysis of expressed Id-1 and VEGF content was performed using Image J program and normalized by the control level. Data are the mean±SEM. *P < 0.05, **P<0.005 (vs. 6–9 weeks group).
Mentions: Next, to examine whether these mRNA expression profiles show the same pattern in the protein level, we carried out western blot analysis for protein expressions of only Id-1 and VEGF because the mRNA expressions of these two factors resulted in a significantly decrease. As shown in RT-PCR experiment, both protein levels of Id-1 and VEGF in the ovary were also significantly decreased with advancing female age (P<0.05). However, unlike mRNA expression results, the expression of Id-1 and VEGF at the protein level began to significantly decrease from the middle age group of 14 ‒ 16 weeks old, and the levels of decrease were more obvious in 23 ‒ 26 weeks old (Fig. 2).

Bottom Line: After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry.Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4.This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.

View Article: PubMed Central - PubMed

Affiliation: Center for Reproductive Medicine, Good Moonhwa Hospital, Busan 601-803, Korea.

ABSTRACT
This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6-9, 14-16, and 23-26 weeks) were injected with 5 IU pregnant mare's serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23-26 weeks compared with the young mice of 6-9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.

No MeSH data available.