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Integration of host strain bioengineering and bioprocess development using ultra-scale down studies to select the optimum combination: an antibody fragment primary recovery case study.

Aucamp JP, Davies R, Hallet D, Weiss A, Titchener-Hooker NJ - Biotechnol. Bioeng. (2014)

Bottom Line: The ability of cells to resist breakage was dependant on a combination of factors including host strain, vector, and fermentation strategy.The use of scale-down primary recovery process sequences can be used to screen a larger number of engineered strains.This can lead to closer integration with and better feedback between strain development, fermentation development, and primary recovery studies.

View Article: PubMed Central - PubMed

Affiliation: Bioprocess Research and Development, Novartis Phama AG, Basel, Switzerland. jean.aucamp@novartis.com.

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Related in: MedlinePlus

Pilot centrifuge and USD capillary discharge results for five feed streams. Feed streams are ranked according to their propensity to break based on product release. Results from CDD can predict relative level of breakage for feed streams evaluated. Error bars represent the standard deviation of results from duplicate USD experiments.
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fig04: Pilot centrifuge and USD capillary discharge results for five feed streams. Feed streams are ranked according to their propensity to break based on product release. Results from CDD can predict relative level of breakage for feed streams evaluated. Error bars represent the standard deviation of results from duplicate USD experiments.

Mentions: The release of product and dsDNA from pilot scale and USD derived heavy phase are compared for several feed streams in Figure 4. The results show that the USD mimic is able to predict the ranking of different feed streams based on susceptibility to damage as measured by either product or dsDNA release. On average two to three times more content was released from the pilot scale discharge than with the USD mimic. The discharge velocity of the disc stack centrifuge at 9,800 rpm was estimated to be 94 m/s with Equations (3) and (4). This suggests that the relative differences in damage between scales is likely due to the differences in discharge velocities.


Integration of host strain bioengineering and bioprocess development using ultra-scale down studies to select the optimum combination: an antibody fragment primary recovery case study.

Aucamp JP, Davies R, Hallet D, Weiss A, Titchener-Hooker NJ - Biotechnol. Bioeng. (2014)

Pilot centrifuge and USD capillary discharge results for five feed streams. Feed streams are ranked according to their propensity to break based on product release. Results from CDD can predict relative level of breakage for feed streams evaluated. Error bars represent the standard deviation of results from duplicate USD experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4282095&req=5

fig04: Pilot centrifuge and USD capillary discharge results for five feed streams. Feed streams are ranked according to their propensity to break based on product release. Results from CDD can predict relative level of breakage for feed streams evaluated. Error bars represent the standard deviation of results from duplicate USD experiments.
Mentions: The release of product and dsDNA from pilot scale and USD derived heavy phase are compared for several feed streams in Figure 4. The results show that the USD mimic is able to predict the ranking of different feed streams based on susceptibility to damage as measured by either product or dsDNA release. On average two to three times more content was released from the pilot scale discharge than with the USD mimic. The discharge velocity of the disc stack centrifuge at 9,800 rpm was estimated to be 94 m/s with Equations (3) and (4). This suggests that the relative differences in damage between scales is likely due to the differences in discharge velocities.

Bottom Line: The ability of cells to resist breakage was dependant on a combination of factors including host strain, vector, and fermentation strategy.The use of scale-down primary recovery process sequences can be used to screen a larger number of engineered strains.This can lead to closer integration with and better feedback between strain development, fermentation development, and primary recovery studies.

View Article: PubMed Central - PubMed

Affiliation: Bioprocess Research and Development, Novartis Phama AG, Basel, Switzerland. jean.aucamp@novartis.com.

Show MeSH
Related in: MedlinePlus