Integration of host strain bioengineering and bioprocess development using ultra-scale down studies to select the optimum combination: an antibody fragment primary recovery case study.
Bottom Line: The ability of cells to resist breakage was dependant on a combination of factors including host strain, vector, and fermentation strategy.The use of scale-down primary recovery process sequences can be used to screen a larger number of engineered strains.This can lead to closer integration with and better feedback between strain development, fermentation development, and primary recovery studies.
Affiliation: Bioprocess Research and Development, Novartis Phama AG, Basel, Switzerland. email@example.com.Show MeSH
Related in: MedlinePlus
Mentions: Pilot scale dewatering (Fig. 3A) and extract clarification (Fig. 3B) were performed for harvested material from several different feed streams and the USD clarification prediction studies conducted in parallel. For both centrifugation steps the USD predictions provided a good estimate of the actual clarification levels obtained in the pilot centrifuge. The USD dewatering level was fixed at 66.7% by removing supernatant equal to two-thirds of total volume before resuspension.
Affiliation: Bioprocess Research and Development, Novartis Phama AG, Basel, Switzerland. firstname.lastname@example.org.