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Establishment and characterization of immortalized bovine endometrial epithelial cells.

Bai H, Sakurai T, Bai R, Yamakoshi S, Aoki E, Kuse M, Okuda K, Imakawa K - Anim. Sci. J. (2014)

Bottom Line: Immortalized bovine EECs were established by transfecting retroviral vectors encoding human papillomavirus (HPV) E6 and E7, and human telomerase reverse transcriptase (hTERT) genes.In addition, imEECs have maintained the features as EECs, exhibiting oxytocin (OT) and interferon tau (IFNT) responsiveness.Therefore, these imEECs, even after numbers of passages, could be used as an in vitro model to investigate cellular and molecular mechanisms, by which the uterine epithelium responds to IFNT stimulation, the event required for the maternal recognition of pregnancy in the bovine species.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Theriogenology and Animal Breeding, Graduate School of Agricultural and Life Science, the University of Tokyo, Tokyo.

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Related in: MedlinePlus

Immunofluorescence characterization of the immortalized endometrial epithelial cell (imEECs). Immunofluorescence staining analysis on primary (left panels) or immortalized at 60th passage (right panels) EECs was carried out using antibodies against cytokeratin (upper panels) as epithelial marker, or vimentin (lower panels) as stromal marker. Bar = 200 μm.
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fig04: Immunofluorescence characterization of the immortalized endometrial epithelial cell (imEECs). Immunofluorescence staining analysis on primary (left panels) or immortalized at 60th passage (right panels) EECs was carried out using antibodies against cytokeratin (upper panels) as epithelial marker, or vimentin (lower panels) as stromal marker. Bar = 200 μm.

Mentions: Immunofluorescence studies were then performed. Primary EECs exhibited positive signals for cytokeratin and negative for vimentin, while imEECs cells stained positive for both cytokeratin and vimentin (Fig. 4).


Establishment and characterization of immortalized bovine endometrial epithelial cells.

Bai H, Sakurai T, Bai R, Yamakoshi S, Aoki E, Kuse M, Okuda K, Imakawa K - Anim. Sci. J. (2014)

Immunofluorescence characterization of the immortalized endometrial epithelial cell (imEECs). Immunofluorescence staining analysis on primary (left panels) or immortalized at 60th passage (right panels) EECs was carried out using antibodies against cytokeratin (upper panels) as epithelial marker, or vimentin (lower panels) as stromal marker. Bar = 200 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4282067&req=5

fig04: Immunofluorescence characterization of the immortalized endometrial epithelial cell (imEECs). Immunofluorescence staining analysis on primary (left panels) or immortalized at 60th passage (right panels) EECs was carried out using antibodies against cytokeratin (upper panels) as epithelial marker, or vimentin (lower panels) as stromal marker. Bar = 200 μm.
Mentions: Immunofluorescence studies were then performed. Primary EECs exhibited positive signals for cytokeratin and negative for vimentin, while imEECs cells stained positive for both cytokeratin and vimentin (Fig. 4).

Bottom Line: Immortalized bovine EECs were established by transfecting retroviral vectors encoding human papillomavirus (HPV) E6 and E7, and human telomerase reverse transcriptase (hTERT) genes.In addition, imEECs have maintained the features as EECs, exhibiting oxytocin (OT) and interferon tau (IFNT) responsiveness.Therefore, these imEECs, even after numbers of passages, could be used as an in vitro model to investigate cellular and molecular mechanisms, by which the uterine epithelium responds to IFNT stimulation, the event required for the maternal recognition of pregnancy in the bovine species.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Theriogenology and Animal Breeding, Graduate School of Agricultural and Life Science, the University of Tokyo, Tokyo.

Show MeSH
Related in: MedlinePlus