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Cancer genomics: one cell at a time.

Navin NE - Genome Biol. (2014)

Bottom Line: The study of single cancer cells has transformed from qualitative microscopic images to quantitative genomic datasets.This paradigm shift has been fueled by the development of single-cell sequencing technologies, which provide a powerful new approach to study complex biological processes in human cancers.

View Article: PubMed Central - PubMed

ABSTRACT
The study of single cancer cells has transformed from qualitative microscopic images to quantitative genomic datasets. This paradigm shift has been fueled by the development of single-cell sequencing technologies, which provide a powerful new approach to study complex biological processes in human cancers.

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Related in: MedlinePlus

Single-cell processes in cancer. Although single cancer cells interact with their neighbors and the adjacent stromal cells, there are many biological processes that occur through the actions of individual cancer cells, shown in this illustration. These complex biological processes in human cancers include: (a) transformation from a single normal somatic cell into a tumor cell; (b) clonal evolution that occurs through a series of selective sweeps when single cells acquire driver mutations and diversify, leading to intratumor heterogeneity; (c) single cells from the primary tumor intravasate into the circulatory system and extravasate at distant organ sites to form metastatic tumors; and (d) the evolution of chemoresistance that occurs when the tumor is eradicated but survived by single tumor cells that harbor resistance mutations and expand to reconstitute the tumor mass.
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Fig1: Single-cell processes in cancer. Although single cancer cells interact with their neighbors and the adjacent stromal cells, there are many biological processes that occur through the actions of individual cancer cells, shown in this illustration. These complex biological processes in human cancers include: (a) transformation from a single normal somatic cell into a tumor cell; (b) clonal evolution that occurs through a series of selective sweeps when single cells acquire driver mutations and diversify, leading to intratumor heterogeneity; (c) single cells from the primary tumor intravasate into the circulatory system and extravasate at distant organ sites to form metastatic tumors; and (d) the evolution of chemoresistance that occurs when the tumor is eradicated but survived by single tumor cells that harbor resistance mutations and expand to reconstitute the tumor mass.

Mentions: While there is substantial evidence that tumor cells can communicate with their neighbors and the stroma, there are also many complex biological processes that occur through the actions of individual cancer cells. These processes include the initial transformation event in a normal cell, clonal expansion within the primary tumor, metastatic dissemination and the evolution of chemoresistance (FigureĀ 1). SCS provides a powerful new approach to study the genomic and transcriptomic basis of these processes directly in human cancers, without the necessity for model organisms.Figure 1


Cancer genomics: one cell at a time.

Navin NE - Genome Biol. (2014)

Single-cell processes in cancer. Although single cancer cells interact with their neighbors and the adjacent stromal cells, there are many biological processes that occur through the actions of individual cancer cells, shown in this illustration. These complex biological processes in human cancers include: (a) transformation from a single normal somatic cell into a tumor cell; (b) clonal evolution that occurs through a series of selective sweeps when single cells acquire driver mutations and diversify, leading to intratumor heterogeneity; (c) single cells from the primary tumor intravasate into the circulatory system and extravasate at distant organ sites to form metastatic tumors; and (d) the evolution of chemoresistance that occurs when the tumor is eradicated but survived by single tumor cells that harbor resistance mutations and expand to reconstitute the tumor mass.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4281948&req=5

Fig1: Single-cell processes in cancer. Although single cancer cells interact with their neighbors and the adjacent stromal cells, there are many biological processes that occur through the actions of individual cancer cells, shown in this illustration. These complex biological processes in human cancers include: (a) transformation from a single normal somatic cell into a tumor cell; (b) clonal evolution that occurs through a series of selective sweeps when single cells acquire driver mutations and diversify, leading to intratumor heterogeneity; (c) single cells from the primary tumor intravasate into the circulatory system and extravasate at distant organ sites to form metastatic tumors; and (d) the evolution of chemoresistance that occurs when the tumor is eradicated but survived by single tumor cells that harbor resistance mutations and expand to reconstitute the tumor mass.
Mentions: While there is substantial evidence that tumor cells can communicate with their neighbors and the stroma, there are also many complex biological processes that occur through the actions of individual cancer cells. These processes include the initial transformation event in a normal cell, clonal expansion within the primary tumor, metastatic dissemination and the evolution of chemoresistance (FigureĀ 1). SCS provides a powerful new approach to study the genomic and transcriptomic basis of these processes directly in human cancers, without the necessity for model organisms.Figure 1

Bottom Line: The study of single cancer cells has transformed from qualitative microscopic images to quantitative genomic datasets.This paradigm shift has been fueled by the development of single-cell sequencing technologies, which provide a powerful new approach to study complex biological processes in human cancers.

View Article: PubMed Central - PubMed

ABSTRACT
The study of single cancer cells has transformed from qualitative microscopic images to quantitative genomic datasets. This paradigm shift has been fueled by the development of single-cell sequencing technologies, which provide a powerful new approach to study complex biological processes in human cancers.

Show MeSH
Related in: MedlinePlus