Evaluating the capacity to generate and preserve nitric oxide bioactivity in highly purified earthworm erythrocruorin: a giant polymeric hemoglobin with potential blood substitute properties.
Bottom Line: A potentially important additional property is the capacity of the HBOC either to generate nitric oxide (NO) or to preserve NO bioactivity to compensate for decreased levels of NO in the circulation.The results show that LtHb undergoes the same reactions as HbA and that the reduced efficacy for these reactions for LtHb relative to HbA is consistent with the much higher redox potential of LtHb.Evidence of functional heterogeneity in LtHb is explained in terms of the large difference in the redox potential of the isolated subunits.
Affiliation: From the Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461.Show MeSH
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Mentions: Figs. 11 and 12 show the evolution of the DAF-FM fluorescence spectra under anaerobic conditions when DAF-FM was added to samples prepared as either the ferrous NO derivative or the NA intermediate of HbA and LtHb. The absorption spectrum was used to establish that in each case the populations were overwhelmingly either ferrous NOHb or the intermediate. For both HbA and LtHb, the increase in fluorescence intensity and the red shift of the fluorescence peak were greater and more rapid for the sample that manifests the absorption spectrum attributable to the NA intermediate (nitrite and NO both added). A similar shift pattern was seen in the absorption maximum of DAF-FM that is also indicative of triazole formation for the samples manifesting the NA intermediate spectrum (not shown).
Affiliation: From the Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461.