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Probiotic properties of Lactobacillus strains isolated from gizzard of local poultry.

Idoui T - Iran J Microbiol (2014)

Bottom Line: The majority of the strains exhibited antagonistic activity towards Escherichia coli, Klebsiella spp., Enterobacter spp., Shigella spp., Salmonella spp and Citrobacter spp.The best co-aggregation properties were obtained with two isolates.Lb. fermentum LP3 alone showed adherence specificity to the chicken intestinal epithelium.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology, Environment and Health, University of Jijel, Algeria.

ABSTRACT

Background and objective: The aim of our study was to characterize gizzard Lactobacillus fermentum-group strains on the basis of their phenotypic profiles regarding characteristics of lactobacilli. In addition, their in vitro potential probiotic properties were evaluated.

Materials and methods: The lactic acid bacteria were isolated and identified from gizzard contents of Algerian local poultry using criteria of Bergey's Manual of Determinative Bacteriology and using methods and criteria of Sharpe. The strains were further characterized by tolerance to low pH and bile, coaggregation potential and adhesion to intestinal mucous. The antagonistic activity against some Enterobacteriaceae strains from poultry origin was also evaluated.

Results and conclusion: Among the strains identified, both physiological and biochemical characteristics differed noticeably. The strains coded LP3 and LP10 survived simulated gastrointestinal conditions and were considered to be acid and bile tolerant. The majority of the strains exhibited antagonistic activity towards Escherichia coli, Klebsiella spp., Enterobacter spp., Shigella spp., Salmonella spp and Citrobacter spp. The best co-aggregation properties were obtained with two isolates. Lb. fermentum LP3 alone showed adherence specificity to the chicken intestinal epithelium.

No MeSH data available.


Related in: MedlinePlus

Adherence of isolated Lb. fermentum on the epithelium cells: (a) negative adhesion (control) (b) positive adhesion of Lb. fermentum LP3.
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Figure 1: Adherence of isolated Lb. fermentum on the epithelium cells: (a) negative adhesion (control) (b) positive adhesion of Lb. fermentum LP3.

Mentions: The ability to adhere to host intestinal mucosa is considered as an important selection criterion for LAB strains intended for probiotic use. It should be reminded that for LAB strain, only more than 15 LAB cells adhered on one epithelial cell, it could be considered as “positive” adherent strain. In our case, we found that Lb. fermentum LP3, LP10, LP11 and LP12 showed the adherence specificity to the chicken intestinal epithelium (Fig. 1).


Probiotic properties of Lactobacillus strains isolated from gizzard of local poultry.

Idoui T - Iran J Microbiol (2014)

Adherence of isolated Lb. fermentum on the epithelium cells: (a) negative adhesion (control) (b) positive adhesion of Lb. fermentum LP3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4281659&req=5

Figure 1: Adherence of isolated Lb. fermentum on the epithelium cells: (a) negative adhesion (control) (b) positive adhesion of Lb. fermentum LP3.
Mentions: The ability to adhere to host intestinal mucosa is considered as an important selection criterion for LAB strains intended for probiotic use. It should be reminded that for LAB strain, only more than 15 LAB cells adhered on one epithelial cell, it could be considered as “positive” adherent strain. In our case, we found that Lb. fermentum LP3, LP10, LP11 and LP12 showed the adherence specificity to the chicken intestinal epithelium (Fig. 1).

Bottom Line: The majority of the strains exhibited antagonistic activity towards Escherichia coli, Klebsiella spp., Enterobacter spp., Shigella spp., Salmonella spp and Citrobacter spp.The best co-aggregation properties were obtained with two isolates.Lb. fermentum LP3 alone showed adherence specificity to the chicken intestinal epithelium.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology, Environment and Health, University of Jijel, Algeria.

ABSTRACT

Background and objective: The aim of our study was to characterize gizzard Lactobacillus fermentum-group strains on the basis of their phenotypic profiles regarding characteristics of lactobacilli. In addition, their in vitro potential probiotic properties were evaluated.

Materials and methods: The lactic acid bacteria were isolated and identified from gizzard contents of Algerian local poultry using criteria of Bergey's Manual of Determinative Bacteriology and using methods and criteria of Sharpe. The strains were further characterized by tolerance to low pH and bile, coaggregation potential and adhesion to intestinal mucous. The antagonistic activity against some Enterobacteriaceae strains from poultry origin was also evaluated.

Results and conclusion: Among the strains identified, both physiological and biochemical characteristics differed noticeably. The strains coded LP3 and LP10 survived simulated gastrointestinal conditions and were considered to be acid and bile tolerant. The majority of the strains exhibited antagonistic activity towards Escherichia coli, Klebsiella spp., Enterobacter spp., Shigella spp., Salmonella spp and Citrobacter spp. The best co-aggregation properties were obtained with two isolates. Lb. fermentum LP3 alone showed adherence specificity to the chicken intestinal epithelium.

No MeSH data available.


Related in: MedlinePlus