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Production and characterization of antimicrobial active substance from Spirulina platensis.

El-Sheekh MM, Daboor SM, Swelim MA, Mohamed S - Iran J Microbiol (2014)

Bottom Line: The results indicated that the IR spectrum showed bands at 1269 cm(-1), 1414 cm(-1) (C-O-C), 1643 cm(-1) (CO of amide),1563 cm(-1) (C = C) and broad band 3441 cm(-1) (of OH and NH)., (1)HNMR showed δ 0.8 (-CH3), δ 1.2 (-CH2), δ 4.2(-OH), δ 7.2(-NH), δ 7.4 and δ 7.7 (aromatic CH)., Mass spectrum showed molecular ion beak at m/z = 341 (abundance (0.03%).Also, the elemental analysis gave molecular formula,C15H18NO8.The results of this investigation proved that cyanobacteria could be a good source for production of antimicrobial agents which could be effective when compared with contemporary antimicrobial compounds.

View Article: PubMed Central - PubMed

Affiliation: Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.

ABSTRACT

Background and objectives: The present work was carried out to investigate the ability of Spirulina platensis to produce antimicrobial substance against bacteria and fungi.

Materials and methods: The cells of the cyanobacterium were subjected to different extractions and the purified antagonistic compound proved to be effective against broad spectrum of bacteria and fungi. The antagonistic compound was purified using thin layer chromatography.

Results: The results indicated that the IR spectrum showed bands at 1269 cm(-1), 1414 cm(-1) (C-O-C), 1643 cm(-1) (CO of amide),1563 cm(-1) (C = C) and broad band 3441 cm(-1) (of OH and NH)., (1)HNMR showed δ 0.8 (-CH3), δ 1.2 (-CH2), δ 4.2(-OH), δ 7.2(-NH), δ 7.4 and δ 7.7 (aromatic CH)., Mass spectrum showed molecular ion beak at m/z = 341 (abundance (0.03%). Also, the elemental analysis gave molecular formula,C15H18NO8.

Conclusion: The purified antimicrobial compound produced by S. platensis was more active against Gram positive, Gram negative bacteria and unicellular fungi, C. albicans. The highest biological activity was recorded against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger. The results of this investigation proved that cyanobacteria could be a good source for production of antimicrobial agents which could be effective when compared with contemporary antimicrobial compounds.

No MeSH data available.


Related in: MedlinePlus

UV Spectrum of the antimicribially active compound produced by Spirulina platensis
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Figure 3: UV Spectrum of the antimicribially active compound produced by Spirulina platensis

Mentions: It was found that the highest biological activity was recorded against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger. The results revealed that diethyl ether and ethyl acetate exhibited antimicrobial activity against Gram +ve and Gram -ve bacteria, while petroleum ether exhibited antimicrobial activity against Gram -ve only and n-hexane had no activity against all test organisms. On the other hand, among the water-miscible solvents (acetone, methanol and ethanol) ethanol was the most effective solvent showed wide spectrum of antimicrobial activity against Gram +ve, Gram -ve bacteria and fungi as shown in Fig.2 and 3.


Production and characterization of antimicrobial active substance from Spirulina platensis.

El-Sheekh MM, Daboor SM, Swelim MA, Mohamed S - Iran J Microbiol (2014)

UV Spectrum of the antimicribially active compound produced by Spirulina platensis
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4281658&req=5

Figure 3: UV Spectrum of the antimicribially active compound produced by Spirulina platensis
Mentions: It was found that the highest biological activity was recorded against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger. The results revealed that diethyl ether and ethyl acetate exhibited antimicrobial activity against Gram +ve and Gram -ve bacteria, while petroleum ether exhibited antimicrobial activity against Gram -ve only and n-hexane had no activity against all test organisms. On the other hand, among the water-miscible solvents (acetone, methanol and ethanol) ethanol was the most effective solvent showed wide spectrum of antimicrobial activity against Gram +ve, Gram -ve bacteria and fungi as shown in Fig.2 and 3.

Bottom Line: The results indicated that the IR spectrum showed bands at 1269 cm(-1), 1414 cm(-1) (C-O-C), 1643 cm(-1) (CO of amide),1563 cm(-1) (C = C) and broad band 3441 cm(-1) (of OH and NH)., (1)HNMR showed δ 0.8 (-CH3), δ 1.2 (-CH2), δ 4.2(-OH), δ 7.2(-NH), δ 7.4 and δ 7.7 (aromatic CH)., Mass spectrum showed molecular ion beak at m/z = 341 (abundance (0.03%).Also, the elemental analysis gave molecular formula,C15H18NO8.The results of this investigation proved that cyanobacteria could be a good source for production of antimicrobial agents which could be effective when compared with contemporary antimicrobial compounds.

View Article: PubMed Central - PubMed

Affiliation: Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.

ABSTRACT

Background and objectives: The present work was carried out to investigate the ability of Spirulina platensis to produce antimicrobial substance against bacteria and fungi.

Materials and methods: The cells of the cyanobacterium were subjected to different extractions and the purified antagonistic compound proved to be effective against broad spectrum of bacteria and fungi. The antagonistic compound was purified using thin layer chromatography.

Results: The results indicated that the IR spectrum showed bands at 1269 cm(-1), 1414 cm(-1) (C-O-C), 1643 cm(-1) (CO of amide),1563 cm(-1) (C = C) and broad band 3441 cm(-1) (of OH and NH)., (1)HNMR showed δ 0.8 (-CH3), δ 1.2 (-CH2), δ 4.2(-OH), δ 7.2(-NH), δ 7.4 and δ 7.7 (aromatic CH)., Mass spectrum showed molecular ion beak at m/z = 341 (abundance (0.03%). Also, the elemental analysis gave molecular formula,C15H18NO8.

Conclusion: The purified antimicrobial compound produced by S. platensis was more active against Gram positive, Gram negative bacteria and unicellular fungi, C. albicans. The highest biological activity was recorded against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger. The results of this investigation proved that cyanobacteria could be a good source for production of antimicrobial agents which could be effective when compared with contemporary antimicrobial compounds.

No MeSH data available.


Related in: MedlinePlus