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Diversity of rotavirus strain circulated in gwangju, republic of Korea.

Kim MJ, Jeong HS, Kim SG, Lee SM, Kim SH, Kee HY, Jo EH, Park HJ, Ha DR, Kim ES, Seo KW, Chung JK - Osong Public Health Res Perspect (2014)

Bottom Line: In this study, nine genotypes were found.Molecular epidemiological knowledge of rotaviruses is critical for the development of effective preventive measures, including vaccines.These data will help us monitor the effectiveness of current rotavirus vaccines.

View Article: PubMed Central - PubMed

Affiliation: Microbiology Division, Health and Environment Research Institute of Gwangju, Gwangju, Korea.

ABSTRACT

Objectives: The introduction of new rotavirus vaccines into the public sphere makes it necessary to maintain constant surveillance and to heighten public awareness of the appearance of new rotavirus strains. We describe the molecular epidemiology of circulating rotavirus strains after vaccine introduction.

Methods: We collected a total of 1070 stool samples from children with gastroenteritis from January 2013 to June 2013. The antigenic prevalence of rotavirus group A was distinguished using enzyme immunoassay. The G and P genotypes of enzyme immunoassay-positive samples were determined with reverse transcription-polymerase chain reaction and nucleotide sequencing analysis.

Results: Of the 1070 samples collected, 277 (25.9%) tested positive for rotaviruses by enzyme-linked immunoabsorbent assay. The most prevalent circulating genotype G was G1 (51.3%), followed by G2 (34.7%) and G9 (10.8%). The predominant type of genotype P was P[8] (66.1%), followed by P[4] (31.4%). In this study, nine genotypes were found. G1P[8] was the most prevalent (51.8%), followed by G2P[4] (30.5%), G9P[8] (9.9%), and G2P[8] (4.0%). Several unusual combinations (G1P[4], G3P[9], G3P[8], G4P[6], and G9P[4]) were also identified.

Conclusion: Molecular epidemiological knowledge of rotaviruses is critical for the development of effective preventive measures, including vaccines. These data will help us monitor the effectiveness of current rotavirus vaccines.

No MeSH data available.


Related in: MedlinePlus

Distribution of major genotypes of rotavirus in Gwangju, with respect to vaccination or nonvaccination groups.
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fig2: Distribution of major genotypes of rotavirus in Gwangju, with respect to vaccination or nonvaccination groups.

Mentions: In total, 272 G and P combinations made up with nine different genotypes were identified. G1P[8], the most frequently detected strain, was responsible for 51.8% (n = 141) of infections. G2P[4], G9P[8], and G2P[8] were detected with a prevalence rate of 30.5% (n = 83), 9.9% (n = 27), and 4.0% (n = 11), respectively. In addition, unusual RV strains were identified, which bore the genotypes of G1P[4], G3P[9], G3P[8], G4P[6], and G9P[4] (Table 3). Among the 67 patients who were vaccinated with RV, the predominant strain was G2P[4] (40.3%), followed by G1P[8] (39.0%) and G9P[8] (12.5%). And among the 389 who were not vaccinated, 144 rotavirus positive samples were analyzed, and the frequencies were as follows: G1P[8] (56.3%), G2P[4] (28.2%), and G9P[8] (9.86%), which were identified with the indicated prevalence (Figure 2).


Diversity of rotavirus strain circulated in gwangju, republic of Korea.

Kim MJ, Jeong HS, Kim SG, Lee SM, Kim SH, Kee HY, Jo EH, Park HJ, Ha DR, Kim ES, Seo KW, Chung JK - Osong Public Health Res Perspect (2014)

Distribution of major genotypes of rotavirus in Gwangju, with respect to vaccination or nonvaccination groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4281628&req=5

fig2: Distribution of major genotypes of rotavirus in Gwangju, with respect to vaccination or nonvaccination groups.
Mentions: In total, 272 G and P combinations made up with nine different genotypes were identified. G1P[8], the most frequently detected strain, was responsible for 51.8% (n = 141) of infections. G2P[4], G9P[8], and G2P[8] were detected with a prevalence rate of 30.5% (n = 83), 9.9% (n = 27), and 4.0% (n = 11), respectively. In addition, unusual RV strains were identified, which bore the genotypes of G1P[4], G3P[9], G3P[8], G4P[6], and G9P[4] (Table 3). Among the 67 patients who were vaccinated with RV, the predominant strain was G2P[4] (40.3%), followed by G1P[8] (39.0%) and G9P[8] (12.5%). And among the 389 who were not vaccinated, 144 rotavirus positive samples were analyzed, and the frequencies were as follows: G1P[8] (56.3%), G2P[4] (28.2%), and G9P[8] (9.86%), which were identified with the indicated prevalence (Figure 2).

Bottom Line: In this study, nine genotypes were found.Molecular epidemiological knowledge of rotaviruses is critical for the development of effective preventive measures, including vaccines.These data will help us monitor the effectiveness of current rotavirus vaccines.

View Article: PubMed Central - PubMed

Affiliation: Microbiology Division, Health and Environment Research Institute of Gwangju, Gwangju, Korea.

ABSTRACT

Objectives: The introduction of new rotavirus vaccines into the public sphere makes it necessary to maintain constant surveillance and to heighten public awareness of the appearance of new rotavirus strains. We describe the molecular epidemiology of circulating rotavirus strains after vaccine introduction.

Methods: We collected a total of 1070 stool samples from children with gastroenteritis from January 2013 to June 2013. The antigenic prevalence of rotavirus group A was distinguished using enzyme immunoassay. The G and P genotypes of enzyme immunoassay-positive samples were determined with reverse transcription-polymerase chain reaction and nucleotide sequencing analysis.

Results: Of the 1070 samples collected, 277 (25.9%) tested positive for rotaviruses by enzyme-linked immunoabsorbent assay. The most prevalent circulating genotype G was G1 (51.3%), followed by G2 (34.7%) and G9 (10.8%). The predominant type of genotype P was P[8] (66.1%), followed by P[4] (31.4%). In this study, nine genotypes were found. G1P[8] was the most prevalent (51.8%), followed by G2P[4] (30.5%), G9P[8] (9.9%), and G2P[8] (4.0%). Several unusual combinations (G1P[4], G3P[9], G3P[8], G4P[6], and G9P[4]) were also identified.

Conclusion: Molecular epidemiological knowledge of rotaviruses is critical for the development of effective preventive measures, including vaccines. These data will help us monitor the effectiveness of current rotavirus vaccines.

No MeSH data available.


Related in: MedlinePlus