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ECR-MAPK regulation in liver early development.

Zhao XJ, Zhuo H - Biomed Res Int (2014)

Bottom Line: Significance analysis of microarrays, qPCR verification, drug induction/inhibition assays, and metabonomics indicated that alpha-2u globulin (extracellular region)-socs2 (-SH2-containing signals/receptor tyrosine kinases)-ppp2r2a/pik3c3 (MAPK signaling)-hsd3b5/cav2 (metabolism/organization) plays a vital role in early development.Taken together, early development of male rats is ECR and MAPK-mediated coordination of cancer-like growth and negative regulations.Our data represent the first comprehensive description of early individual development, which could be a valuable basis for understanding the functioning of the gene interaction network of infant development.

View Article: PubMed Central - PubMed

Affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Collaborative Innovation Center of Processing of Agricultural Products in Hubei Province, No. 68 South Xuefu Road, Changqing Garden, Wuhan 430023, China ; Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, West No. 30 Xiaohongshan, Wuhan 430071, China.

ABSTRACT
Early growth is connected to a key link between embryonic development and aging. In this paper, liver gene expression profiles were assayed at postnatal day 22 and week 16 of age. Meanwhile another independent animal experiment and cell culture were carried out for validation. Significance analysis of microarrays, qPCR verification, drug induction/inhibition assays, and metabonomics indicated that alpha-2u globulin (extracellular region)-socs2 (-SH2-containing signals/receptor tyrosine kinases)-ppp2r2a/pik3c3 (MAPK signaling)-hsd3b5/cav2 (metabolism/organization) plays a vital role in early development. Taken together, early development of male rats is ECR and MAPK-mediated coordination of cancer-like growth and negative regulations. Our data represent the first comprehensive description of early individual development, which could be a valuable basis for understanding the functioning of the gene interaction network of infant development.

Show MeSH
ECR-MAPK-mediated early individual development network. Extracellular region and space (ECR) act as nutrition ligand and information input. Ligands interact with membrane transports and SH2-containing/MAPK related signals and regulate cell cycle, transcription, and proteolysis, leading to short-term steroid, fatty acid biosynthesis, redox and metabolic process, and long-term collagen development and organization. G protein coupled receptors/G protein, catalytic receptors, and ECR signals converge at MAPK cascades.
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fig4: ECR-MAPK-mediated early individual development network. Extracellular region and space (ECR) act as nutrition ligand and information input. Ligands interact with membrane transports and SH2-containing/MAPK related signals and regulate cell cycle, transcription, and proteolysis, leading to short-term steroid, fatty acid biosynthesis, redox and metabolic process, and long-term collagen development and organization. G protein coupled receptors/G protein, catalytic receptors, and ECR signals converge at MAPK cascades.

Mentions: Based on gene profiling, verification at mRNA, protein, and metabolite levels, we postulated that, in early development, extracellular region and space (ECR) obp3, rup2, pcdh17, a2m, and cxcl13 act as nutrition ligand and information input. Ligands interact with membrane transports ust5r, cdh17, mme, olr59, gpm6a, tmem163, abcg8, abcd2, abcc3, and SH2-containing/MAPK related signals stac3, socs2, cish/pik3c3, and nrg1 and regulate cell cycle, transcription, and proteolysis ccng2, ccnb2, ccna2/zfp37, zfp68, npas2, taf9b, ppargc1a, nfe2/hspb1, usp18, mup5, mme, trhde, spink3, and prcp, leading to short-term steroid, fatty acid biosynthesis, redox, and metabolic process obp3, hsd3b5, akr1c1, hsdl2, hsd17b6, hsd11b2, ar, cyp17a1/scd, fasn/cyp2c13, cyp3a9, cyp2a2, hao2, cyp2c29, nox4, me3, cyp2c12, akr1b7/dhrs7, mett17b, asns, acsm2, psat1 and long-term collagen development and organization col4a1, col5a2, col5a1, col1a2, col1a1/cav2, pex11a, onecut1, meox2, cml3, and lox. G protein coupled receptors/G protein olr59, rgs3 [22], adora2b/gnai3, gnat3, catalytic receptors socs2 [23], nim1 [24], atp6ap2, ghr and ECR signals converge at MAPK cascades (Figure 4). Protein expression to some extent confirmed key genes, for example, obp3, socs2, ppp2r2a, pik3c3, cxcl13, and hsd3b5 proteins dynamics (Figure 2).


ECR-MAPK regulation in liver early development.

Zhao XJ, Zhuo H - Biomed Res Int (2014)

ECR-MAPK-mediated early individual development network. Extracellular region and space (ECR) act as nutrition ligand and information input. Ligands interact with membrane transports and SH2-containing/MAPK related signals and regulate cell cycle, transcription, and proteolysis, leading to short-term steroid, fatty acid biosynthesis, redox and metabolic process, and long-term collagen development and organization. G protein coupled receptors/G protein, catalytic receptors, and ECR signals converge at MAPK cascades.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4281454&req=5

fig4: ECR-MAPK-mediated early individual development network. Extracellular region and space (ECR) act as nutrition ligand and information input. Ligands interact with membrane transports and SH2-containing/MAPK related signals and regulate cell cycle, transcription, and proteolysis, leading to short-term steroid, fatty acid biosynthesis, redox and metabolic process, and long-term collagen development and organization. G protein coupled receptors/G protein, catalytic receptors, and ECR signals converge at MAPK cascades.
Mentions: Based on gene profiling, verification at mRNA, protein, and metabolite levels, we postulated that, in early development, extracellular region and space (ECR) obp3, rup2, pcdh17, a2m, and cxcl13 act as nutrition ligand and information input. Ligands interact with membrane transports ust5r, cdh17, mme, olr59, gpm6a, tmem163, abcg8, abcd2, abcc3, and SH2-containing/MAPK related signals stac3, socs2, cish/pik3c3, and nrg1 and regulate cell cycle, transcription, and proteolysis ccng2, ccnb2, ccna2/zfp37, zfp68, npas2, taf9b, ppargc1a, nfe2/hspb1, usp18, mup5, mme, trhde, spink3, and prcp, leading to short-term steroid, fatty acid biosynthesis, redox, and metabolic process obp3, hsd3b5, akr1c1, hsdl2, hsd17b6, hsd11b2, ar, cyp17a1/scd, fasn/cyp2c13, cyp3a9, cyp2a2, hao2, cyp2c29, nox4, me3, cyp2c12, akr1b7/dhrs7, mett17b, asns, acsm2, psat1 and long-term collagen development and organization col4a1, col5a2, col5a1, col1a2, col1a1/cav2, pex11a, onecut1, meox2, cml3, and lox. G protein coupled receptors/G protein olr59, rgs3 [22], adora2b/gnai3, gnat3, catalytic receptors socs2 [23], nim1 [24], atp6ap2, ghr and ECR signals converge at MAPK cascades (Figure 4). Protein expression to some extent confirmed key genes, for example, obp3, socs2, ppp2r2a, pik3c3, cxcl13, and hsd3b5 proteins dynamics (Figure 2).

Bottom Line: Significance analysis of microarrays, qPCR verification, drug induction/inhibition assays, and metabonomics indicated that alpha-2u globulin (extracellular region)-socs2 (-SH2-containing signals/receptor tyrosine kinases)-ppp2r2a/pik3c3 (MAPK signaling)-hsd3b5/cav2 (metabolism/organization) plays a vital role in early development.Taken together, early development of male rats is ECR and MAPK-mediated coordination of cancer-like growth and negative regulations.Our data represent the first comprehensive description of early individual development, which could be a valuable basis for understanding the functioning of the gene interaction network of infant development.

View Article: PubMed Central - PubMed

Affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Collaborative Innovation Center of Processing of Agricultural Products in Hubei Province, No. 68 South Xuefu Road, Changqing Garden, Wuhan 430023, China ; Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, West No. 30 Xiaohongshan, Wuhan 430071, China.

ABSTRACT
Early growth is connected to a key link between embryonic development and aging. In this paper, liver gene expression profiles were assayed at postnatal day 22 and week 16 of age. Meanwhile another independent animal experiment and cell culture were carried out for validation. Significance analysis of microarrays, qPCR verification, drug induction/inhibition assays, and metabonomics indicated that alpha-2u globulin (extracellular region)-socs2 (-SH2-containing signals/receptor tyrosine kinases)-ppp2r2a/pik3c3 (MAPK signaling)-hsd3b5/cav2 (metabolism/organization) plays a vital role in early development. Taken together, early development of male rats is ECR and MAPK-mediated coordination of cancer-like growth and negative regulations. Our data represent the first comprehensive description of early individual development, which could be a valuable basis for understanding the functioning of the gene interaction network of infant development.

Show MeSH