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Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo.

Cui E, Zhi X, Chen Y, Gao Y, Fan Y, Zhang W, Ma W, Hou W, Guo C, Song X - Evid Based Complement Alternat Med (2014)

Bottom Line: Methods.At 150-18.75 μg·mL(-1), CM had no cytotoxicity and could significantly promote the growth and the phagocytosis of macrophages and inhibit the overproduction of NO, iNOS, TNF-α, and IL-6 in macrophages induced by LPS.Our results demonstrated that Coptis chinensis and myrobalan possessed synergistically anti-inflammatory activities in vitro and in vivo, which indicated that CM had therapeutic potential for the prevention and treatment of inflammation-mediated diseases.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Objectives. To investigate the anti-inflammatory effect of Coptis chinensis plus myrobalan (CM) in vitro and in vivo. Methods. The inflammation in mouse peritoneal macrophages was induced by lipopolysaccharide (LPS). Animal models were established by using ear swelling and paw edema of mouse induced by xylene and formaldehyde, respectively. In vitro, cytotoxicity, the phagocytosis of macrophages, the levels of nitric oxide (NO), induced nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in cell supernatant were detected. In vivo, swelling rate and edema inhibitory rate of ear and paw were observed using CM-treated mice. Results. At 150-18.75 μg·mL(-1), CM had no cytotoxicity and could significantly promote the growth and the phagocytosis of macrophages and inhibit the overproduction of NO, iNOS, TNF-α, and IL-6 in macrophages induced by LPS. In vivo, pretreatment with CM, the ear swelling, and paw edema of mice could be significantly inhibited in a dose-dependent manner, and the antiedema effect of CM at high dose was better than dexamethasone. Conclusion. Our results demonstrated that Coptis chinensis and myrobalan possessed synergistically anti-inflammatory activities in vitro and in vivo, which indicated that CM had therapeutic potential for the prevention and treatment of inflammation-mediated diseases.

No MeSH data available.


Related in: MedlinePlus

Effect of CM on xylene induced ear swelling in mice. a-bBars without the same superscripts differ significantly (P < 0.05). H: high dose; M: medium dose; L: low dose; BC: blank control.
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fig8: Effect of CM on xylene induced ear swelling in mice. a-bBars without the same superscripts differ significantly (P < 0.05). H: high dose; M: medium dose; L: low dose; BC: blank control.

Mentions: Figure 8 shows the swelling rate and inhibitory rate of CM in formaldehyde induced paw edema in mice. The results demonstrated that the swelling rates of paw in CMH, CMM, CML, and dexamethasone groups were all lower than that in BC group; CMH group was the lowest and significantly lower than BC group (P < 0.05). The inhibitory rates of paw in CMH, CMM, CML, and dexamethasone groups were all higher than that in BC group; CMH group was the highest and significantly higher than those in CMM, CML, and BC groups (P < 0.05). There was no significant difference between CMH and dexamethasone groups (P > 0.05).


Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo.

Cui E, Zhi X, Chen Y, Gao Y, Fan Y, Zhang W, Ma W, Hou W, Guo C, Song X - Evid Based Complement Alternat Med (2014)

Effect of CM on xylene induced ear swelling in mice. a-bBars without the same superscripts differ significantly (P < 0.05). H: high dose; M: medium dose; L: low dose; BC: blank control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4281439&req=5

fig8: Effect of CM on xylene induced ear swelling in mice. a-bBars without the same superscripts differ significantly (P < 0.05). H: high dose; M: medium dose; L: low dose; BC: blank control.
Mentions: Figure 8 shows the swelling rate and inhibitory rate of CM in formaldehyde induced paw edema in mice. The results demonstrated that the swelling rates of paw in CMH, CMM, CML, and dexamethasone groups were all lower than that in BC group; CMH group was the lowest and significantly lower than BC group (P < 0.05). The inhibitory rates of paw in CMH, CMM, CML, and dexamethasone groups were all higher than that in BC group; CMH group was the highest and significantly higher than those in CMM, CML, and BC groups (P < 0.05). There was no significant difference between CMH and dexamethasone groups (P > 0.05).

Bottom Line: Methods.At 150-18.75 μg·mL(-1), CM had no cytotoxicity and could significantly promote the growth and the phagocytosis of macrophages and inhibit the overproduction of NO, iNOS, TNF-α, and IL-6 in macrophages induced by LPS.Our results demonstrated that Coptis chinensis and myrobalan possessed synergistically anti-inflammatory activities in vitro and in vivo, which indicated that CM had therapeutic potential for the prevention and treatment of inflammation-mediated diseases.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Objectives. To investigate the anti-inflammatory effect of Coptis chinensis plus myrobalan (CM) in vitro and in vivo. Methods. The inflammation in mouse peritoneal macrophages was induced by lipopolysaccharide (LPS). Animal models were established by using ear swelling and paw edema of mouse induced by xylene and formaldehyde, respectively. In vitro, cytotoxicity, the phagocytosis of macrophages, the levels of nitric oxide (NO), induced nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in cell supernatant were detected. In vivo, swelling rate and edema inhibitory rate of ear and paw were observed using CM-treated mice. Results. At 150-18.75 μg·mL(-1), CM had no cytotoxicity and could significantly promote the growth and the phagocytosis of macrophages and inhibit the overproduction of NO, iNOS, TNF-α, and IL-6 in macrophages induced by LPS. In vivo, pretreatment with CM, the ear swelling, and paw edema of mice could be significantly inhibited in a dose-dependent manner, and the antiedema effect of CM at high dose was better than dexamethasone. Conclusion. Our results demonstrated that Coptis chinensis and myrobalan possessed synergistically anti-inflammatory activities in vitro and in vivo, which indicated that CM had therapeutic potential for the prevention and treatment of inflammation-mediated diseases.

No MeSH data available.


Related in: MedlinePlus