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Adult neurogenesis in the four-striped mice (Rhabdomys pumilio).

Olaleye OO, Ihunwo AO - Neural Regen Res (2014)

Bottom Line: Ki-67 (a marker for cell proliferation) and doublecortin (a marker for immature neurons) immunostaining confirmed that adult neurogenesis occurs in the active sites of subventricular zone of the lateral ventricle with the migratory stream to the olfactory bulb, and the subgranular zone of the dentate gyrus of the hippocampus.No Ki-67 proliferating cells were observed in the striatum substantia nigra, amygdala, cerebral cortex or dorsal vagal complex.Doublecortin-immunoreactive cells were observed in the striatum, third ventricle, cerebral cortex, amygdala, olfactory bulb and along the rostral migratory stream but absent in the substantia nigra and dorsal vagal complex.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Biological Sciences, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa ; School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

ABSTRACT
In this study, we investigated non-captive four-striped mice (Rhabdomys pumilio) for evidence that adult neurogenesis occurs in the adult brain of animal models in natural environment. Ki-67 (a marker for cell proliferation) and doublecortin (a marker for immature neurons) immunostaining confirmed that adult neurogenesis occurs in the active sites of subventricular zone of the lateral ventricle with the migratory stream to the olfactory bulb, and the subgranular zone of the dentate gyrus of the hippocampus. No Ki-67 proliferating cells were observed in the striatum substantia nigra, amygdala, cerebral cortex or dorsal vagal complex. Doublecortin-immunoreactive cells were observed in the striatum, third ventricle, cerebral cortex, amygdala, olfactory bulb and along the rostral migratory stream but absent in the substantia nigra and dorsal vagal complex. The potential neurogenic sites in the four-striped mouse species could invariably lead to increased neural plasticity.

No MeSH data available.


Related in: MedlinePlus

Representative photomicrograph of Ki-67 immunohistochemical staining in the various brain regions of four-striped mice.(A) DG; (B) subventricular zone and RMS. (C) A magnified image of the RMS showing the darkly stained neurons. Scale bars: 20 μm in A, 10 μm in B and 1 μm in C. DG: Dentate gyrus; RMS: rostral migratory stream; hil: hilum; LV: lateral ventricle.
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Figure 1: Representative photomicrograph of Ki-67 immunohistochemical staining in the various brain regions of four-striped mice.(A) DG; (B) subventricular zone and RMS. (C) A magnified image of the RMS showing the darkly stained neurons. Scale bars: 20 μm in A, 10 μm in B and 1 μm in C. DG: Dentate gyrus; RMS: rostral migratory stream; hil: hilum; LV: lateral ventricle.

Mentions: Ki-67-immunoreactive cells appeared darkly stained and were easily seen in various sites of the brain (Figure 1A–C). The Ki-67-immunoreactive cells appeared more numerous in the subventricular layer of the dentate gyrus. These cells were present in the two established neurogenic sites; subventricular zone and the dentate gyrus (Figure 1A, B). Also, Ki-67-immunoreactive neurons were visible in the rostral migratory stream and olfactory bulb (Figure 1C). No Ki-67-immunoreactive cells were observed in the striatum, amygdala, cerebral cortex or dorsal vagal complex.


Adult neurogenesis in the four-striped mice (Rhabdomys pumilio).

Olaleye OO, Ihunwo AO - Neural Regen Res (2014)

Representative photomicrograph of Ki-67 immunohistochemical staining in the various brain regions of four-striped mice.(A) DG; (B) subventricular zone and RMS. (C) A magnified image of the RMS showing the darkly stained neurons. Scale bars: 20 μm in A, 10 μm in B and 1 μm in C. DG: Dentate gyrus; RMS: rostral migratory stream; hil: hilum; LV: lateral ventricle.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4281430&req=5

Figure 1: Representative photomicrograph of Ki-67 immunohistochemical staining in the various brain regions of four-striped mice.(A) DG; (B) subventricular zone and RMS. (C) A magnified image of the RMS showing the darkly stained neurons. Scale bars: 20 μm in A, 10 μm in B and 1 μm in C. DG: Dentate gyrus; RMS: rostral migratory stream; hil: hilum; LV: lateral ventricle.
Mentions: Ki-67-immunoreactive cells appeared darkly stained and were easily seen in various sites of the brain (Figure 1A–C). The Ki-67-immunoreactive cells appeared more numerous in the subventricular layer of the dentate gyrus. These cells were present in the two established neurogenic sites; subventricular zone and the dentate gyrus (Figure 1A, B). Also, Ki-67-immunoreactive neurons were visible in the rostral migratory stream and olfactory bulb (Figure 1C). No Ki-67-immunoreactive cells were observed in the striatum, amygdala, cerebral cortex or dorsal vagal complex.

Bottom Line: Ki-67 (a marker for cell proliferation) and doublecortin (a marker for immature neurons) immunostaining confirmed that adult neurogenesis occurs in the active sites of subventricular zone of the lateral ventricle with the migratory stream to the olfactory bulb, and the subgranular zone of the dentate gyrus of the hippocampus.No Ki-67 proliferating cells were observed in the striatum substantia nigra, amygdala, cerebral cortex or dorsal vagal complex.Doublecortin-immunoreactive cells were observed in the striatum, third ventricle, cerebral cortex, amygdala, olfactory bulb and along the rostral migratory stream but absent in the substantia nigra and dorsal vagal complex.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Biological Sciences, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa ; School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

ABSTRACT
In this study, we investigated non-captive four-striped mice (Rhabdomys pumilio) for evidence that adult neurogenesis occurs in the adult brain of animal models in natural environment. Ki-67 (a marker for cell proliferation) and doublecortin (a marker for immature neurons) immunostaining confirmed that adult neurogenesis occurs in the active sites of subventricular zone of the lateral ventricle with the migratory stream to the olfactory bulb, and the subgranular zone of the dentate gyrus of the hippocampus. No Ki-67 proliferating cells were observed in the striatum substantia nigra, amygdala, cerebral cortex or dorsal vagal complex. Doublecortin-immunoreactive cells were observed in the striatum, third ventricle, cerebral cortex, amygdala, olfactory bulb and along the rostral migratory stream but absent in the substantia nigra and dorsal vagal complex. The potential neurogenic sites in the four-striped mouse species could invariably lead to increased neural plasticity.

No MeSH data available.


Related in: MedlinePlus