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Effects of low level laser treatment on the survival of axotomized retinal ganglion cells in adult Hamsters.

So KF, Leung MC, Cui Q - Neural Regen Res (2014)

Bottom Line: Injury to axons close to the neuronal bodies in the mammalian central nervous system causes a large proportion of parenting neurons to degenerate.We also found that single dose and early commencement of laser irradiation were important in protecting retinal ganglion cells following optic nerve axotomy.These findings thus convincingly show that appropriate laser treatment may be neuroprotective to retinal ganglion cells.

View Article: PubMed Central - PubMed

Affiliation: GHM Institute of CNS Regeneration, and Guangdong Key Laboratory of Brain Function and Diseases, Jinan University, Guangzhou, Guangdong Province, China ; Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China ; Department of Ophthalmology, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.

ABSTRACT
Injury to axons close to the neuronal bodies in the mammalian central nervous system causes a large proportion of parenting neurons to degenerate. It is known that optic nerve transection close to the eye in rodents leads to a loss of about half of retinal ganglion cells in 1 week and about 90% in 2 weeks. Using low level laser treatment in the present study, we demonstrated that treatment with helium-neon (660 nm) laser with 15 mW power could delay retinal ganglion cell death after optic nerve axotomy in adult hamsters. The effect was most apparent in the first week with a short period of treatment time (5 minutes) in which 65-66% of retinal ganglion cells survived the optic nerve axotomy whereas 45-47% of retinal ganglion cells did so in optic nerve axotomy controls. We also found that single dose and early commencement of laser irradiation were important in protecting retinal ganglion cells following optic nerve axotomy. These findings thus convincingly show that appropriate laser treatment may be neuroprotective to retinal ganglion cells.

No MeSH data available.


Related in: MedlinePlus

Effects of various low level laser irradiation treatment regimes on the survival of retinal ganglion cells (RGCs) after optic nerve (ON) axotomy at 7 and 14 days post-axotomy in hamsters.1-min: 1-minute laser treatment; 5-min: 5-minute laser treatment; 10-min: 10-minute laser treatment. Data are expressed as the mean ± SD. *P < 0.05, ***P < 0.001 (one-way analysis of variance followed by Bonferroni multiple comparison test).
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Figure 3: Effects of various low level laser irradiation treatment regimes on the survival of retinal ganglion cells (RGCs) after optic nerve (ON) axotomy at 7 and 14 days post-axotomy in hamsters.1-min: 1-minute laser treatment; 5-min: 5-minute laser treatment; 10-min: 10-minute laser treatment. Data are expressed as the mean ± SD. *P < 0.05, ***P < 0.001 (one-way analysis of variance followed by Bonferroni multiple comparison test).

Mentions: Figure 2 shows the characteristics of photomicrographs of GB-labeled RGCs under different experimental conditions. The average number of GB-labeled RGCs in the GCL of normal hamsters (n = 6) was 78,711 ± 2,314 (Figure 3). After ON transection, the average number of RGCs was decreased to 35,945 ± 1,871/retina (n = 6) at 7 days post-axotomy and 10,608 ± 406/retina (n = 5) at 14 days post-axotomy (Figure 3). These were about 55% and 87% reductions in the normal RGC number. These results are consistent with previous reports that ON axotomy close to the eye led to a loss of about half of RGC population within 1 week and about 90% in 2 weeks (Berkelaar et al., 1994).


Effects of low level laser treatment on the survival of axotomized retinal ganglion cells in adult Hamsters.

So KF, Leung MC, Cui Q - Neural Regen Res (2014)

Effects of various low level laser irradiation treatment regimes on the survival of retinal ganglion cells (RGCs) after optic nerve (ON) axotomy at 7 and 14 days post-axotomy in hamsters.1-min: 1-minute laser treatment; 5-min: 5-minute laser treatment; 10-min: 10-minute laser treatment. Data are expressed as the mean ± SD. *P < 0.05, ***P < 0.001 (one-way analysis of variance followed by Bonferroni multiple comparison test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4281419&req=5

Figure 3: Effects of various low level laser irradiation treatment regimes on the survival of retinal ganglion cells (RGCs) after optic nerve (ON) axotomy at 7 and 14 days post-axotomy in hamsters.1-min: 1-minute laser treatment; 5-min: 5-minute laser treatment; 10-min: 10-minute laser treatment. Data are expressed as the mean ± SD. *P < 0.05, ***P < 0.001 (one-way analysis of variance followed by Bonferroni multiple comparison test).
Mentions: Figure 2 shows the characteristics of photomicrographs of GB-labeled RGCs under different experimental conditions. The average number of GB-labeled RGCs in the GCL of normal hamsters (n = 6) was 78,711 ± 2,314 (Figure 3). After ON transection, the average number of RGCs was decreased to 35,945 ± 1,871/retina (n = 6) at 7 days post-axotomy and 10,608 ± 406/retina (n = 5) at 14 days post-axotomy (Figure 3). These were about 55% and 87% reductions in the normal RGC number. These results are consistent with previous reports that ON axotomy close to the eye led to a loss of about half of RGC population within 1 week and about 90% in 2 weeks (Berkelaar et al., 1994).

Bottom Line: Injury to axons close to the neuronal bodies in the mammalian central nervous system causes a large proportion of parenting neurons to degenerate.We also found that single dose and early commencement of laser irradiation were important in protecting retinal ganglion cells following optic nerve axotomy.These findings thus convincingly show that appropriate laser treatment may be neuroprotective to retinal ganglion cells.

View Article: PubMed Central - PubMed

Affiliation: GHM Institute of CNS Regeneration, and Guangdong Key Laboratory of Brain Function and Diseases, Jinan University, Guangzhou, Guangdong Province, China ; Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China ; Department of Ophthalmology, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.

ABSTRACT
Injury to axons close to the neuronal bodies in the mammalian central nervous system causes a large proportion of parenting neurons to degenerate. It is known that optic nerve transection close to the eye in rodents leads to a loss of about half of retinal ganglion cells in 1 week and about 90% in 2 weeks. Using low level laser treatment in the present study, we demonstrated that treatment with helium-neon (660 nm) laser with 15 mW power could delay retinal ganglion cell death after optic nerve axotomy in adult hamsters. The effect was most apparent in the first week with a short period of treatment time (5 minutes) in which 65-66% of retinal ganglion cells survived the optic nerve axotomy whereas 45-47% of retinal ganglion cells did so in optic nerve axotomy controls. We also found that single dose and early commencement of laser irradiation were important in protecting retinal ganglion cells following optic nerve axotomy. These findings thus convincingly show that appropriate laser treatment may be neuroprotective to retinal ganglion cells.

No MeSH data available.


Related in: MedlinePlus