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Neurochemical mechanism of the gastrointestinal interdigestive migrating motor complex in rats with acute inflammatory stomach ache.

Xu X, Li Q, Zhou L, Ru L - Neural Regen Res (2012)

Bottom Line: Activities of nitric oxide synthase, acetylcholinesterase and vasoactive intestinal peptide neurons were significantly reduced, whereas activities of calcitonin gene-related peptide neurons were significantly increased in the pyloric sphincter muscular layer, myenteric nerve plexus and submucous nerve plexus.Electroacupuncture at Zusanli (ST36) suppressed paroxysmal contraction in rats with formaldehyde-induced stomach ache, and neurons in the enteric nervous system were normal.These results indicated that nitrergic neurons, cholinergic neurons, vasoactive intestinal peptide neurons and calcitonin gene-related peptide neurons in the enteric nervous system may be involved in changes to the gastrointestinal interdigestive migrating motor complex following stomach ache, and that electroacupuncture can regulate this process.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, School of Medicine, Jianghan University, Wuhan 430056, Hubei Province, China.

ABSTRACT
The normal gastrointestinal interdigestive migrating motor complex cycle was interrupted, and paroxysmal contraction appeared after formaldehyde-induced stomach ache. Activities of nitric oxide synthase, acetylcholinesterase and vasoactive intestinal peptide neurons were significantly reduced, whereas activities of calcitonin gene-related peptide neurons were significantly increased in the pyloric sphincter muscular layer, myenteric nerve plexus and submucous nerve plexus. Electroacupuncture at Zusanli (ST36) suppressed paroxysmal contraction in rats with formaldehyde-induced stomach ache, and neurons in the enteric nervous system were normal. These results indicated that nitrergic neurons, cholinergic neurons, vasoactive intestinal peptide neurons and calcitonin gene-related peptide neurons in the enteric nervous system may be involved in changes to the gastrointestinal interdigestive migrating motor complex following stomach ache, and that electroacupuncture can regulate this process.

No MeSH data available.


Related in: MedlinePlus

Effect of electroacupuncture on nitric oxide synthase (NOS)-positive neurons in rats with gastric inflammatory pain (NADPH staining, light microscope; scale bar: 40 μm). Arrows exhibit positive neurons.Compared with the normal control group (A) and electroacupuncture control group (C), the number of NOS-positive perikarya and enzymatic activity were lower in the submucosal plexus and myenteric nerve plexus of the sphincter in the model group (B). Above-mentioned data in the electroacupuncture treatment group (D) were near to the normal control group.
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Figure 3: Effect of electroacupuncture on nitric oxide synthase (NOS)-positive neurons in rats with gastric inflammatory pain (NADPH staining, light microscope; scale bar: 40 μm). Arrows exhibit positive neurons.Compared with the normal control group (A) and electroacupuncture control group (C), the number of NOS-positive perikarya and enzymatic activity were lower in the submucosal plexus and myenteric nerve plexus of the sphincter in the model group (B). Above-mentioned data in the electroacupuncture treatment group (D) were near to the normal control group.

Mentions: Histological detection revealed that the sphincter was surrounded by circular muscle bundles where there were four to seven NOS-positive nerve plexuses and one to five NOS-positive perikarya in each plexus. In thickened circular muscle bundles, there were abundant NOS-positive perikarya, 5–12 perikarya in each plexus, and strong enzymatic activity (+++). Abundant NOS-positive fibers and moderate enzymatic activity (++) was visible in the muscular layer, but only a few NOS-positive fibers were found in the lamina propria and mucosa. AChE-positive ganglia (or nerve plexuses) of unequal size and number were observed in the muscularis propria. AChE-positive perikarya were observed, and enzymatic activity was uneven and mainly moderate (++). In the thickened muscular layer, we found five to six plexuses and each plexus had at least 10 cells with a high enzymatic activity (++ – +++). The distribution of AChE-positive fibers was similar to that of NOS. In the sphincter, the number of VIP-immunoreactive nerve fibers was more than CGRP-immunoreactive fibers. In the thickened site of the sphincter, VIP-immunoreactive nerve fibers formed a dense fasciculate structure, whereas CGRP-immunoreactive fibers were scattered (Figures 3–6).


Neurochemical mechanism of the gastrointestinal interdigestive migrating motor complex in rats with acute inflammatory stomach ache.

Xu X, Li Q, Zhou L, Ru L - Neural Regen Res (2012)

Effect of electroacupuncture on nitric oxide synthase (NOS)-positive neurons in rats with gastric inflammatory pain (NADPH staining, light microscope; scale bar: 40 μm). Arrows exhibit positive neurons.Compared with the normal control group (A) and electroacupuncture control group (C), the number of NOS-positive perikarya and enzymatic activity were lower in the submucosal plexus and myenteric nerve plexus of the sphincter in the model group (B). Above-mentioned data in the electroacupuncture treatment group (D) were near to the normal control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4281416&req=5

Figure 3: Effect of electroacupuncture on nitric oxide synthase (NOS)-positive neurons in rats with gastric inflammatory pain (NADPH staining, light microscope; scale bar: 40 μm). Arrows exhibit positive neurons.Compared with the normal control group (A) and electroacupuncture control group (C), the number of NOS-positive perikarya and enzymatic activity were lower in the submucosal plexus and myenteric nerve plexus of the sphincter in the model group (B). Above-mentioned data in the electroacupuncture treatment group (D) were near to the normal control group.
Mentions: Histological detection revealed that the sphincter was surrounded by circular muscle bundles where there were four to seven NOS-positive nerve plexuses and one to five NOS-positive perikarya in each plexus. In thickened circular muscle bundles, there were abundant NOS-positive perikarya, 5–12 perikarya in each plexus, and strong enzymatic activity (+++). Abundant NOS-positive fibers and moderate enzymatic activity (++) was visible in the muscular layer, but only a few NOS-positive fibers were found in the lamina propria and mucosa. AChE-positive ganglia (or nerve plexuses) of unequal size and number were observed in the muscularis propria. AChE-positive perikarya were observed, and enzymatic activity was uneven and mainly moderate (++). In the thickened muscular layer, we found five to six plexuses and each plexus had at least 10 cells with a high enzymatic activity (++ – +++). The distribution of AChE-positive fibers was similar to that of NOS. In the sphincter, the number of VIP-immunoreactive nerve fibers was more than CGRP-immunoreactive fibers. In the thickened site of the sphincter, VIP-immunoreactive nerve fibers formed a dense fasciculate structure, whereas CGRP-immunoreactive fibers were scattered (Figures 3–6).

Bottom Line: Activities of nitric oxide synthase, acetylcholinesterase and vasoactive intestinal peptide neurons were significantly reduced, whereas activities of calcitonin gene-related peptide neurons were significantly increased in the pyloric sphincter muscular layer, myenteric nerve plexus and submucous nerve plexus.Electroacupuncture at Zusanli (ST36) suppressed paroxysmal contraction in rats with formaldehyde-induced stomach ache, and neurons in the enteric nervous system were normal.These results indicated that nitrergic neurons, cholinergic neurons, vasoactive intestinal peptide neurons and calcitonin gene-related peptide neurons in the enteric nervous system may be involved in changes to the gastrointestinal interdigestive migrating motor complex following stomach ache, and that electroacupuncture can regulate this process.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, School of Medicine, Jianghan University, Wuhan 430056, Hubei Province, China.

ABSTRACT
The normal gastrointestinal interdigestive migrating motor complex cycle was interrupted, and paroxysmal contraction appeared after formaldehyde-induced stomach ache. Activities of nitric oxide synthase, acetylcholinesterase and vasoactive intestinal peptide neurons were significantly reduced, whereas activities of calcitonin gene-related peptide neurons were significantly increased in the pyloric sphincter muscular layer, myenteric nerve plexus and submucous nerve plexus. Electroacupuncture at Zusanli (ST36) suppressed paroxysmal contraction in rats with formaldehyde-induced stomach ache, and neurons in the enteric nervous system were normal. These results indicated that nitrergic neurons, cholinergic neurons, vasoactive intestinal peptide neurons and calcitonin gene-related peptide neurons in the enteric nervous system may be involved in changes to the gastrointestinal interdigestive migrating motor complex following stomach ache, and that electroacupuncture can regulate this process.

No MeSH data available.


Related in: MedlinePlus