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Cell hydration as a biomarker for estimation of biological effects of nonionizing radiation on cells and organisms.

Ayrapetyan S, De J - ScientificWorldJournal (2014)

Bottom Line: "Changes in cell hydration" have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR).To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed.Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

View Article: PubMed Central - PubMed

Affiliation: UNESCO Chair in Life Sciences International Postgraduate Educational Center, 31 Acharian Street, 0040 Yerevan, Armenia.

ABSTRACT
"Changes in cell hydration" have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR). To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed. In dormant state temperature sensitivity (Q 10) of seed hydration in distilled water (DW) was less than 2, and it was nonsensitive to NIR treated DW, whereas during the germination period (48-72 hours) seeds hydration exhibited temperature sensitivity Q 10 > 2 and higher sensitivity to NIR treated DW. Obtained data allow us to suggest that the metabolic driving of intracellular water dynamics accompanied by hydrogen bonding and breaking is more sensitive to NIR-induced water structure changes in seed bathing aqua medium than the simple thermodynamic processes such as osmotic gradient driven water absorption by seeds in dormant state. Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

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Related in: MedlinePlus

Time-dependent changes in seed hydration during 72 hours of incubation in ELF EMF (15 Hz) treated (gray column) and nontreated Sham (clear column) DW in cold (4°C; clear/grey with no pattern markings) and warm (20°C; clear/grey with horizontal pattern markings) conditions. The time (in hours) of seeds incubation on abscissa and the value of seed hydration (mg of H2O for 1 mg of dry weight) on ordinate are presented. The results are shown as mean ± SEM (n = 300) from three independent experiments, 100 seeds from each experiment. ∗P < 0.05, compared with Sham, ∗∗P < 0.01.
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fig7: Time-dependent changes in seed hydration during 72 hours of incubation in ELF EMF (15 Hz) treated (gray column) and nontreated Sham (clear column) DW in cold (4°C; clear/grey with no pattern markings) and warm (20°C; clear/grey with horizontal pattern markings) conditions. The time (in hours) of seeds incubation on abscissa and the value of seed hydration (mg of H2O for 1 mg of dry weight) on ordinate are presented. The results are shown as mean ± SEM (n = 300) from three independent experiments, 100 seeds from each experiment. ∗P < 0.05, compared with Sham, ∗∗P < 0.01.

Mentions: As can be seen in Figure 7 the 15 Hz ELF EMF treated DW had activation effect on seed hydration. Such activation effect was more expressed at the end of 72 hours of incubation at room temperature. It is worth to note that EMF sensitivity at room temperature was not expressed until the first 48 hours (so as at 4°C), whereas it was significantly higher at 72 hours of incubation. These data clearly indicate that the EMF-induced elevation of seed hydration was due to the metabolic processes corresponding to the time of incubation and those were activated in the course of germination. It is necessary to note that the latter can be considered as a significant increase in water content. The Q10 was 1.22 for water absorption in cold DW, whereas the same at room temperature was 2.70. These data showed that the metabolism-dependent seed hydration was more sensitive to EMF-induced water structure changes than the metabolism-independent one (in cold DW and during the first 2 hours of incubation in warm DW).


Cell hydration as a biomarker for estimation of biological effects of nonionizing radiation on cells and organisms.

Ayrapetyan S, De J - ScientificWorldJournal (2014)

Time-dependent changes in seed hydration during 72 hours of incubation in ELF EMF (15 Hz) treated (gray column) and nontreated Sham (clear column) DW in cold (4°C; clear/grey with no pattern markings) and warm (20°C; clear/grey with horizontal pattern markings) conditions. The time (in hours) of seeds incubation on abscissa and the value of seed hydration (mg of H2O for 1 mg of dry weight) on ordinate are presented. The results are shown as mean ± SEM (n = 300) from three independent experiments, 100 seeds from each experiment. ∗P < 0.05, compared with Sham, ∗∗P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4281404&req=5

fig7: Time-dependent changes in seed hydration during 72 hours of incubation in ELF EMF (15 Hz) treated (gray column) and nontreated Sham (clear column) DW in cold (4°C; clear/grey with no pattern markings) and warm (20°C; clear/grey with horizontal pattern markings) conditions. The time (in hours) of seeds incubation on abscissa and the value of seed hydration (mg of H2O for 1 mg of dry weight) on ordinate are presented. The results are shown as mean ± SEM (n = 300) from three independent experiments, 100 seeds from each experiment. ∗P < 0.05, compared with Sham, ∗∗P < 0.01.
Mentions: As can be seen in Figure 7 the 15 Hz ELF EMF treated DW had activation effect on seed hydration. Such activation effect was more expressed at the end of 72 hours of incubation at room temperature. It is worth to note that EMF sensitivity at room temperature was not expressed until the first 48 hours (so as at 4°C), whereas it was significantly higher at 72 hours of incubation. These data clearly indicate that the EMF-induced elevation of seed hydration was due to the metabolic processes corresponding to the time of incubation and those were activated in the course of germination. It is necessary to note that the latter can be considered as a significant increase in water content. The Q10 was 1.22 for water absorption in cold DW, whereas the same at room temperature was 2.70. These data showed that the metabolism-dependent seed hydration was more sensitive to EMF-induced water structure changes than the metabolism-independent one (in cold DW and during the first 2 hours of incubation in warm DW).

Bottom Line: "Changes in cell hydration" have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR).To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed.Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

View Article: PubMed Central - PubMed

Affiliation: UNESCO Chair in Life Sciences International Postgraduate Educational Center, 31 Acharian Street, 0040 Yerevan, Armenia.

ABSTRACT
"Changes in cell hydration" have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR). To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed. In dormant state temperature sensitivity (Q 10) of seed hydration in distilled water (DW) was less than 2, and it was nonsensitive to NIR treated DW, whereas during the germination period (48-72 hours) seeds hydration exhibited temperature sensitivity Q 10 > 2 and higher sensitivity to NIR treated DW. Obtained data allow us to suggest that the metabolic driving of intracellular water dynamics accompanied by hydrogen bonding and breaking is more sensitive to NIR-induced water structure changes in seed bathing aqua medium than the simple thermodynamic processes such as osmotic gradient driven water absorption by seeds in dormant state. Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

Show MeSH
Related in: MedlinePlus