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Cell hydration as a biomarker for estimation of biological effects of nonionizing radiation on cells and organisms.

Ayrapetyan S, De J - ScientificWorldJournal (2014)

Bottom Line: "Changes in cell hydration" have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR).To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed.Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

View Article: PubMed Central - PubMed

Affiliation: UNESCO Chair in Life Sciences International Postgraduate Educational Center, 31 Acharian Street, 0040 Yerevan, Armenia.

ABSTRACT
"Changes in cell hydration" have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR). To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed. In dormant state temperature sensitivity (Q 10) of seed hydration in distilled water (DW) was less than 2, and it was nonsensitive to NIR treated DW, whereas during the germination period (48-72 hours) seeds hydration exhibited temperature sensitivity Q 10 > 2 and higher sensitivity to NIR treated DW. Obtained data allow us to suggest that the metabolic driving of intracellular water dynamics accompanied by hydrogen bonding and breaking is more sensitive to NIR-induced water structure changes in seed bathing aqua medium than the simple thermodynamic processes such as osmotic gradient driven water absorption by seeds in dormant state. Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

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Related in: MedlinePlus

Time-dependent changes in seed hydration during 72 hours of incubation in nontreated (“C” = Sham) DW in cold (4°C; (A) clear symbol) and warm (20°C; (B) dark symbol) conditions. S = initial reading at 5 minutes after the start of the incubation. In the present and the following figures: “C” means Control-Sham. The time (in hours) of seeds incubation on abscissa and the value of seed hydration (mg of H2O for 1 mg of dry weight) on ordinate are presented. The results are shown as mean ± SEM (n = 300) from three independent experiments, 100 seeds from each experiment. ∗P < 0.05, compared with Sham, ∗∗P < 0.01.
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fig3: Time-dependent changes in seed hydration during 72 hours of incubation in nontreated (“C” = Sham) DW in cold (4°C; (A) clear symbol) and warm (20°C; (B) dark symbol) conditions. S = initial reading at 5 minutes after the start of the incubation. In the present and the following figures: “C” means Control-Sham. The time (in hours) of seeds incubation on abscissa and the value of seed hydration (mg of H2O for 1 mg of dry weight) on ordinate are presented. The results are shown as mean ± SEM (n = 300) from three independent experiments, 100 seeds from each experiment. ∗P < 0.05, compared with Sham, ∗∗P < 0.01.

Mentions: The temperature sensitivity (Q10 coefficient) of the rate of processes serves as a marker for detecting whether they have diffusion or metabolic nature; that is, they are determined by Fick's law (Q10 < 2) and Arrhenius's equation (Q10 > 2), respectively. The time-dependent temperature sensitivity of seeds hydration incubated in DW in cold (4°C) and at room (20°C) temperature is shown in Figure 3.


Cell hydration as a biomarker for estimation of biological effects of nonionizing radiation on cells and organisms.

Ayrapetyan S, De J - ScientificWorldJournal (2014)

Time-dependent changes in seed hydration during 72 hours of incubation in nontreated (“C” = Sham) DW in cold (4°C; (A) clear symbol) and warm (20°C; (B) dark symbol) conditions. S = initial reading at 5 minutes after the start of the incubation. In the present and the following figures: “C” means Control-Sham. The time (in hours) of seeds incubation on abscissa and the value of seed hydration (mg of H2O for 1 mg of dry weight) on ordinate are presented. The results are shown as mean ± SEM (n = 300) from three independent experiments, 100 seeds from each experiment. ∗P < 0.05, compared with Sham, ∗∗P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4281404&req=5

fig3: Time-dependent changes in seed hydration during 72 hours of incubation in nontreated (“C” = Sham) DW in cold (4°C; (A) clear symbol) and warm (20°C; (B) dark symbol) conditions. S = initial reading at 5 minutes after the start of the incubation. In the present and the following figures: “C” means Control-Sham. The time (in hours) of seeds incubation on abscissa and the value of seed hydration (mg of H2O for 1 mg of dry weight) on ordinate are presented. The results are shown as mean ± SEM (n = 300) from three independent experiments, 100 seeds from each experiment. ∗P < 0.05, compared with Sham, ∗∗P < 0.01.
Mentions: The temperature sensitivity (Q10 coefficient) of the rate of processes serves as a marker for detecting whether they have diffusion or metabolic nature; that is, they are determined by Fick's law (Q10 < 2) and Arrhenius's equation (Q10 > 2), respectively. The time-dependent temperature sensitivity of seeds hydration incubated in DW in cold (4°C) and at room (20°C) temperature is shown in Figure 3.

Bottom Line: "Changes in cell hydration" have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR).To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed.Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

View Article: PubMed Central - PubMed

Affiliation: UNESCO Chair in Life Sciences International Postgraduate Educational Center, 31 Acharian Street, 0040 Yerevan, Armenia.

ABSTRACT
"Changes in cell hydration" have been hypothesized as an input signal for intracellular metabolic cascade responsible for biological effects of nonionizing radiation (NIR). To test this hypothesis a comparative study on the impacts of different temperature and NIR (infrasound frequency mechanical vibration (MV), static magnetic field (SMF), extremely low frequency electromagnetic field (ELF EMF), and microwave (MW)) pretreated water on the hydration of barley seeds in its dormant and germination periods was performed. In dormant state temperature sensitivity (Q 10) of seed hydration in distilled water (DW) was less than 2, and it was nonsensitive to NIR treated DW, whereas during the germination period (48-72 hours) seeds hydration exhibited temperature sensitivity Q 10 > 2 and higher sensitivity to NIR treated DW. Obtained data allow us to suggest that the metabolic driving of intracellular water dynamics accompanied by hydrogen bonding and breaking is more sensitive to NIR-induced water structure changes in seed bathing aqua medium than the simple thermodynamic processes such as osmotic gradient driven water absorption by seeds in dormant state. Therefore, cell hydration is suggested to be a universal and extrasensitive biomarker for detection of biological effects of NIR on cells and organisms.

Show MeSH
Related in: MedlinePlus