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Uptake of the antisecretory factor peptide AF-16 in rat blood and cerebrospinal fluid and effects on elevated intracranial pressure.

Al-Olama M, Lange S, Lönnroth I, Gatzinsky K, Jennische E - Acta Neurochir (Wien) (2014)

Bottom Line: The continuous ICP registrations were achieved by means of telemetry.Intranasal administration of AF-16 suppressed the increased ICP to normal values within 30 min.The ability of AF-16 to suppress an increased ICP was manifested.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, P.O.B. 440, 405 30, Göteborg, Sweden.

ABSTRACT

Background: AF-16 is a 16-amino-acid-long peptide derived from the amino-terminal part of the endogenous protein, antisecretory factor (AF). AF-16 in vivo has been shown to regulate dysfunctions in the water and ion transport system under various pathological conditions and also to counteract experimentally increased tissue pressure.

Methods: Rats were subjected to a cryogenic brain injury in order to increase the intracranial pressure (ICP). The distribution of AF-16 in blood and CSF after intravenous or intranasal administration was determined in injured and control rats. ICP was monitored in freely moving, awake rats, by means of an epidural pressure transducer catheter connected to a wireless device placed subcutaneously on the skull. The continuous ICP registrations were achieved by means of telemetry.

Results: Intranasal administration of AF-16 resulted in a significantly higher CSF concentrations of AF-16 in injured than in control rats, 1.3 versus 0.6 ng/ml, whereas no difference between injured and control rats was seen when AF-16 was given intravenously. Rats subjected to cryogenic brain injury developed gradually increasing ICP levels. Intranasal administration of AF-16 suppressed the increased ICP to normal values within 30 min.

Conclusion: Optimal AF-16 concentrations in CSF are achieved after intranasal administration in rats subjected to a cryogenic brain injury. The ability of AF-16 to suppress an increased ICP was manifested.

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Related in: MedlinePlus

Paraffin section from the brain of an injured rat showing a typical superficial, rounded lesion. Cresyl violet. Scale bar 500 μm
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Fig6: Paraffin section from the brain of an injured rat showing a typical superficial, rounded lesion. Cresyl violet. Scale bar 500 μm

Mentions: The mean lesion volume, calculated on the assumption that the lesion was shaped as a half sphere was 12.2 ± 1.8 mm3 (n = 20). Morphological examination of the cryogenic damage to the brain showed that the injury appeared as a superficial, rounded necrosis in the parietal cortex. The histology of a typical lesion is shown in Fig. 6.Fig. 6


Uptake of the antisecretory factor peptide AF-16 in rat blood and cerebrospinal fluid and effects on elevated intracranial pressure.

Al-Olama M, Lange S, Lönnroth I, Gatzinsky K, Jennische E - Acta Neurochir (Wien) (2014)

Paraffin section from the brain of an injured rat showing a typical superficial, rounded lesion. Cresyl violet. Scale bar 500 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4281356&req=5

Fig6: Paraffin section from the brain of an injured rat showing a typical superficial, rounded lesion. Cresyl violet. Scale bar 500 μm
Mentions: The mean lesion volume, calculated on the assumption that the lesion was shaped as a half sphere was 12.2 ± 1.8 mm3 (n = 20). Morphological examination of the cryogenic damage to the brain showed that the injury appeared as a superficial, rounded necrosis in the parietal cortex. The histology of a typical lesion is shown in Fig. 6.Fig. 6

Bottom Line: The continuous ICP registrations were achieved by means of telemetry.Intranasal administration of AF-16 suppressed the increased ICP to normal values within 30 min.The ability of AF-16 to suppress an increased ICP was manifested.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, P.O.B. 440, 405 30, Göteborg, Sweden.

ABSTRACT

Background: AF-16 is a 16-amino-acid-long peptide derived from the amino-terminal part of the endogenous protein, antisecretory factor (AF). AF-16 in vivo has been shown to regulate dysfunctions in the water and ion transport system under various pathological conditions and also to counteract experimentally increased tissue pressure.

Methods: Rats were subjected to a cryogenic brain injury in order to increase the intracranial pressure (ICP). The distribution of AF-16 in blood and CSF after intravenous or intranasal administration was determined in injured and control rats. ICP was monitored in freely moving, awake rats, by means of an epidural pressure transducer catheter connected to a wireless device placed subcutaneously on the skull. The continuous ICP registrations were achieved by means of telemetry.

Results: Intranasal administration of AF-16 resulted in a significantly higher CSF concentrations of AF-16 in injured than in control rats, 1.3 versus 0.6 ng/ml, whereas no difference between injured and control rats was seen when AF-16 was given intravenously. Rats subjected to cryogenic brain injury developed gradually increasing ICP levels. Intranasal administration of AF-16 suppressed the increased ICP to normal values within 30 min.

Conclusion: Optimal AF-16 concentrations in CSF are achieved after intranasal administration in rats subjected to a cryogenic brain injury. The ability of AF-16 to suppress an increased ICP was manifested.

Show MeSH
Related in: MedlinePlus