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Increase in the rate of azithromycin-resistant Streptococcus pneumoniae isolates carrying the erm(B) and mef(A) genes in Taiwan, 2006-2010.

Safari D, Kuo LC, Huang YT, Liao CH, Sheng WH, Hsueh PR - BMC Infect. Dis. (2014)

Bottom Line: The prevalence of isolates carrying the erm(B) and mef(A) genes increased from 10% (11/110) in 2006 to 25% (15/60) in 2010 (p-value = 0.0136).The majority of isolates carrying both erm(B) and mef(A) genes belonged to serotypes 19 F (64%) followed by 19 F A (24%).Of these isolates, 33% were sequence type 320 (ST320), 32% were ST236, and 12% were ST271.

View Article: PubMed Central - PubMed

Affiliation: Eijkman Institute for Molecular Biology, Jakarta, Indonesia. dodi.safari@gmail.com.

ABSTRACT

Background: This study investigated the molecular characteristics of azithromycin-resistant Streptococcus pneumoniae in Taiwan.

Methods: A total of 486 non-duplicate isolates of azithromycin-resistant S. pneumoniae recovered from various clinical sources of patients treated at 22 different hospitals in Taiwan from 2006 to 2010. The presence of erm(B) and mef(A) genes using duplex PCR, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis of these isolates were studied.

Results: Of the isolates tested, 59% carried the erm(B) gene, 22% carried the mef(A) gene, and 19% carried both genes. The prevalence of isolates carrying the erm(B) and mef(A) genes increased from 10% (11/110) in 2006 to 25% (15/60) in 2010 (p-value = 0.0136). The majority of isolates carrying both erm(B) and mef(A) genes belonged to serotypes 19 F (64%) followed by 19 F A (24%). Of these isolates, 33% were sequence type 320 (ST320), 32% were ST236, and 12% were ST271.

Conclusions: The increase in incidence of mef(A)/erm(B)-positive azithromycin-resistant S. pneumoniae isolates during the study period was primarily due to serotypes 19 F and 19A and ST236 and ST320.

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A phylogenetic tree analysis based on pulsed-field gel electrophoresis profiles withSmaI among isolates of azithromycin-resistantStreptococcus pneumoniaecarrying botherm(B)/mef(A)genes.
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Fig1: A phylogenetic tree analysis based on pulsed-field gel electrophoresis profiles withSmaI among isolates of azithromycin-resistantStreptococcus pneumoniaecarrying botherm(B)/mef(A)genes.

Mentions: We constructed a phylogenetic tree based on PFGE profiles and found no specific clustering for the strains of serotype 19 F and 19A or for the three major sequence types (ST320, ST271, and ST236) (Figure 1). In this study, the isolates carrying both erm(B) and mef(A) were stratified into eight clusters (Cluster I to VIII) by PFGE (Figure 1 and Table 4). Clusters III, IV, V, and VII corresponded to the isolates with serotype 19 F (Table 4). Isolates belonging to the same cluster can have different serotypes and STs. Furthermore, several isolates with the same ST also exhibited different serotypes and pulsotypes. The majority of isolates of serotype 19A were in cluster VI (13/24, 54%). ST320 clone isolates belonged to cluster VI (13/24, 54%), cluster II (9/16, 56%), and cluster I (2/4, 50%). Meanwhile, isolates of clone ST236 were frequently clustered in cluster V (9/9, 100%) and III (6/7, 86%) (Table 4). The majority of ST81 clone isolates were clustered in cluster VIII (4/5, 80%).Figure 1


Increase in the rate of azithromycin-resistant Streptococcus pneumoniae isolates carrying the erm(B) and mef(A) genes in Taiwan, 2006-2010.

Safari D, Kuo LC, Huang YT, Liao CH, Sheng WH, Hsueh PR - BMC Infect. Dis. (2014)

A phylogenetic tree analysis based on pulsed-field gel electrophoresis profiles withSmaI among isolates of azithromycin-resistantStreptococcus pneumoniaecarrying botherm(B)/mef(A)genes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4279982&req=5

Fig1: A phylogenetic tree analysis based on pulsed-field gel electrophoresis profiles withSmaI among isolates of azithromycin-resistantStreptococcus pneumoniaecarrying botherm(B)/mef(A)genes.
Mentions: We constructed a phylogenetic tree based on PFGE profiles and found no specific clustering for the strains of serotype 19 F and 19A or for the three major sequence types (ST320, ST271, and ST236) (Figure 1). In this study, the isolates carrying both erm(B) and mef(A) were stratified into eight clusters (Cluster I to VIII) by PFGE (Figure 1 and Table 4). Clusters III, IV, V, and VII corresponded to the isolates with serotype 19 F (Table 4). Isolates belonging to the same cluster can have different serotypes and STs. Furthermore, several isolates with the same ST also exhibited different serotypes and pulsotypes. The majority of isolates of serotype 19A were in cluster VI (13/24, 54%). ST320 clone isolates belonged to cluster VI (13/24, 54%), cluster II (9/16, 56%), and cluster I (2/4, 50%). Meanwhile, isolates of clone ST236 were frequently clustered in cluster V (9/9, 100%) and III (6/7, 86%) (Table 4). The majority of ST81 clone isolates were clustered in cluster VIII (4/5, 80%).Figure 1

Bottom Line: The prevalence of isolates carrying the erm(B) and mef(A) genes increased from 10% (11/110) in 2006 to 25% (15/60) in 2010 (p-value = 0.0136).The majority of isolates carrying both erm(B) and mef(A) genes belonged to serotypes 19 F (64%) followed by 19 F A (24%).Of these isolates, 33% were sequence type 320 (ST320), 32% were ST236, and 12% were ST271.

View Article: PubMed Central - PubMed

Affiliation: Eijkman Institute for Molecular Biology, Jakarta, Indonesia. dodi.safari@gmail.com.

ABSTRACT

Background: This study investigated the molecular characteristics of azithromycin-resistant Streptococcus pneumoniae in Taiwan.

Methods: A total of 486 non-duplicate isolates of azithromycin-resistant S. pneumoniae recovered from various clinical sources of patients treated at 22 different hospitals in Taiwan from 2006 to 2010. The presence of erm(B) and mef(A) genes using duplex PCR, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis of these isolates were studied.

Results: Of the isolates tested, 59% carried the erm(B) gene, 22% carried the mef(A) gene, and 19% carried both genes. The prevalence of isolates carrying the erm(B) and mef(A) genes increased from 10% (11/110) in 2006 to 25% (15/60) in 2010 (p-value = 0.0136). The majority of isolates carrying both erm(B) and mef(A) genes belonged to serotypes 19 F (64%) followed by 19 F A (24%). Of these isolates, 33% were sequence type 320 (ST320), 32% were ST236, and 12% were ST271.

Conclusions: The increase in incidence of mef(A)/erm(B)-positive azithromycin-resistant S. pneumoniae isolates during the study period was primarily due to serotypes 19 F and 19A and ST236 and ST320.

Show MeSH
Related in: MedlinePlus