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Profile of usage of a reference diagnostic service on oral pathology: a 10-year evaluation.

Oliveira e Silva KR, Siqueira AL, Caldeira PC, de Abreu MH, de Aguiar MC - BMC Health Serv Res (2014)

Bottom Line: The Public Health System of the city of Belo Horizonte was the main requestor of exams (77.13%), followed by private professionals (19.26%), and other cities (2.03%).The most used antibodies were against S100, vimentin, smooth muscle actin, actin muscle specific HHF-35, and pan-cytokeratin AE1/AE3.Immunohistochemistry was particularly important in solving challenging cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Oral Surgery, School of Dentistry, Universidade Federal de Minas, Gerais, Brazil. karla_rachel_oliveira@hotmail.com.

ABSTRACT

Background: Despite the professional and academic relevance of the Brazilian oral pathology diagnostic laboratories, no information about their usage profile is available in the English literature. The objective of the present study is to report data about the histopathological and immunohistochemical exams performed in a Brazilian regional reference laboratory of oral pathology, as well as its main users.

Methods: Information about all histopathological exams performed between 2002 and 2012 was retrieved from the files of the Oral and Maxillofacial Pathology Service of the School of Dentistry of Universidade Federal de Minas Gerais. Data collected included: 1) requestor of exam; 2) diagnosis classification; and 3) immunohistochemical tests. Descriptive statistical analyses were done.

Results: 13,522 histopathological exams were performed, mean 1,229/year. The Public Health System of the city of Belo Horizonte was the main requestor of exams (77.13%), followed by private professionals (19.26%), and other cities (2.03%). Most lesions were considered benign (12,599/ 93.17%), with 854 malignant lesions (6.32%). 469 immunohistochemical tests were performed; 324 (69.08%) were from benign diagnosis, and 145 (30.92%) from malignant diagnosis. The most used antibodies were against S100, vimentin, smooth muscle actin, actin muscle specific HHF-35, and pan-cytokeratin AE1/AE3.

Conclusions: Public Health System is the major user of the diagnostic service on oral pathology in our institution. Most diagnoses were of benign lesions, although many malignant lesions were detected. Immunohistochemistry was particularly important in solving challenging cases.

No MeSH data available.


Related in: MedlinePlus

Antibodies’ specificity used in immunohistochemistry.
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Fig4: Antibodies’ specificity used in immunohistochemistry.

Mentions: Forty-five different antibodies were used in immunohistochemical tests (Table 3). The most used ones were against S100 (65/13.86%), vimentin (51/10.87%), smooth muscle actin (34/7.24%), actin muscle specific HHF-35 (31/6.60%), and AE1/AE3 (28/5.97%). Figure 4 shows the types of antibodies used, grouped according to their specificity.Table 3


Profile of usage of a reference diagnostic service on oral pathology: a 10-year evaluation.

Oliveira e Silva KR, Siqueira AL, Caldeira PC, de Abreu MH, de Aguiar MC - BMC Health Serv Res (2014)

Antibodies’ specificity used in immunohistochemistry.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4279906&req=5

Fig4: Antibodies’ specificity used in immunohistochemistry.
Mentions: Forty-five different antibodies were used in immunohistochemical tests (Table 3). The most used ones were against S100 (65/13.86%), vimentin (51/10.87%), smooth muscle actin (34/7.24%), actin muscle specific HHF-35 (31/6.60%), and AE1/AE3 (28/5.97%). Figure 4 shows the types of antibodies used, grouped according to their specificity.Table 3

Bottom Line: The Public Health System of the city of Belo Horizonte was the main requestor of exams (77.13%), followed by private professionals (19.26%), and other cities (2.03%).The most used antibodies were against S100, vimentin, smooth muscle actin, actin muscle specific HHF-35, and pan-cytokeratin AE1/AE3.Immunohistochemistry was particularly important in solving challenging cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Oral Surgery, School of Dentistry, Universidade Federal de Minas, Gerais, Brazil. karla_rachel_oliveira@hotmail.com.

ABSTRACT

Background: Despite the professional and academic relevance of the Brazilian oral pathology diagnostic laboratories, no information about their usage profile is available in the English literature. The objective of the present study is to report data about the histopathological and immunohistochemical exams performed in a Brazilian regional reference laboratory of oral pathology, as well as its main users.

Methods: Information about all histopathological exams performed between 2002 and 2012 was retrieved from the files of the Oral and Maxillofacial Pathology Service of the School of Dentistry of Universidade Federal de Minas Gerais. Data collected included: 1) requestor of exam; 2) diagnosis classification; and 3) immunohistochemical tests. Descriptive statistical analyses were done.

Results: 13,522 histopathological exams were performed, mean 1,229/year. The Public Health System of the city of Belo Horizonte was the main requestor of exams (77.13%), followed by private professionals (19.26%), and other cities (2.03%). Most lesions were considered benign (12,599/ 93.17%), with 854 malignant lesions (6.32%). 469 immunohistochemical tests were performed; 324 (69.08%) were from benign diagnosis, and 145 (30.92%) from malignant diagnosis. The most used antibodies were against S100, vimentin, smooth muscle actin, actin muscle specific HHF-35, and pan-cytokeratin AE1/AE3.

Conclusions: Public Health System is the major user of the diagnostic service on oral pathology in our institution. Most diagnoses were of benign lesions, although many malignant lesions were detected. Immunohistochemistry was particularly important in solving challenging cases.

No MeSH data available.


Related in: MedlinePlus